Systemic Administration of Interleukin-10 Attenuates Early Ischemic Response Following Spinal Cord Ischemia Reperfusion Injury in Rats

• Published in: The Journal of surgical research Vol. 155; no. 2; pp. 345 - 356
• Main Authors: Oruckaptan, H. Hakan, M.D., Ph.D, Ozisik, Pinar, M.D., Ph.D, Atilla, Pergin, M.D, Tuncel, Muruvet, M.D, Kilinc, Kamer, M.D, Geyik, Pinar Ozdemir, Ph.D, Basaran, Nursen, M.D, Yüksel, Eser, M.D, Ozcan, O. Ekin, M.D
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-08-2009; Elsevier
• Subjects: Animals; Biological and medical sciences; central nervous system; Cerebrospinal fluid. Meninges. Spinal cord; cytokine; DNA Fragmentation - drug effects; Endothelium - metabolism; Endothelium - pathology; Endothelium - ultrastructure; Female; General aspects; inflammation; interleukin-10; Interleukin-10 - pharmacology; Interleukin-10 - therapeutic use; Interleukin-1beta - antagonists & inhibitors; interleukin-1β; ischemia; lipid peroxidation; Lipid Peroxidation - drug effects; Malondialdehyde - metabolism; Medical sciences; myeloperoxidase; Nervous system (semeiology, syndromes); Neurology; Neurons - metabolism; Neurons - pathology; Neurons - ultrastructure; Peroxidase - metabolism; Rats; Rats, Mutant Strains; reperfusion injury; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Reperfusion Injury - prevention & control; spinal cord; Spinal Cord Ischemia - metabolism; Spinal Cord Ischemia - pathology; Spinal Cord Ischemia - prevention & control; Surgery; Treatment Outcome; cytokine; inflammation; reperfusion injury; myeloperoxidase; spinal cord; interleukin-10; lipid peroxidation; ischemia; interleukin-1β; central nervous system; Spinal cord; Rat; Central nervous system; Cardiovascular disease; Lipids; Systemic; Vascular disease; Reperfusion; Spinal cord trauma; Disseminated; Peroxidation; Nervous system diseases; Rodentia; Cytokine; Interleukin 1β; Inflammation; Spinal cord ischemia; Vertebrata; Mammalia; Treatment; Animal; Central nervous system disease; Interleukin 10; Early; Lesion; Spinal cord disease
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2008.07.013

Background The aim of this experimental study was to investigate the early effects of interleukin-10 (IL-10) and interleukin-1β antagonist (anti-IL-1β) against cellular damage, inflammatory reactivity, lipid peroxidation (LPO), and myeloperoxidase (MPO) activity induced by spinal cord ischemia reperfusion injury (IRI). Methods Thirty-two single strain female Albino rats were divided into four groups: control (sham-operated), IRI-alone, IL-10-treated (100 μg/kg), and anti-IL-1β-treated (1 mg/kg) groups after IRI. IRI was induced by balloon occlusion of the aorta and simultaneous hypovolemia during occlusion. The animals were sacrificed at 24 h. Histopathological and ultrastructural analyses, biochemical studies for determination of LPO and MPO activity and Comet assays (single cell electrophoresis for detecting DNA single strand breaks) were performed in all study groups. Results Compared with the levels of control (sham-operated) animals, IRI produced a significant increase in the levels of LPO and MPO activity, and prominent tissue damage characterized by leukocyte infiltration, edema and neuronal and glial damage in the affected spinal cord in 24 h. The administration of IL-10 decreased LPO and MPO activity, and suppressed initial inflammatory response in the first 24 h. The effects of anti-IL-1β were limited to decrease in LPO activity without considerable evidence of cellular preservation. Conclusions These data suggest that systemic administration of IL-10 attenuates the early ischemic response, and may restrict the tissue damage in the first 24 h after spinal cord ischemia reperfusion injury. Anti-IL-1β has no considerable effect in this time window. The results of this preliminary study promote further studies with longer time windows on the effects of anti-inflammatory cytokines in spinal cord IRI.