Cooperation and Functional Diversification of Two Closely Related Galactolipase Genes for Jasmonate Biosynthesis

• Published in: Developmental cell Vol. 14; no. 2; pp. 183 - 192
• Main Authors: Hyun, Youbong, Choi, Sungwook, Hwang, Hyun-Ju, Yu, Jihyeon, Nam, Sang-Jip, Ko, Jaeyoung, Park, Ju-Young, Seo, Young Sam, Kim, Eun Yu, Ryu, Stephen Beungtae, Kim, Woo Taek, Lee, Yong-Hwan, Kang, Heonjoong, Lee, Ilha
• Format: Journal Article
• Language: English
• Published: Cambridge, MA Elsevier Inc 01-02-2008; Cell Press
• Subjects: Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - ultrastructure; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Biological and medical sciences; Carboxylic Ester Hydrolases - genetics; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Chloroplasts - metabolism; Cyclopentanes - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Genes, Plant; Genetic Variation; Molecular and cellular biology; Organ Specificity; Oxylipins - metabolism; Phenotype; Phospholipase D - metabolism; Phospholipases A - metabolism; Phospholipases A1 - metabolism; Plant Leaves - growth & development; Plant Leaves - metabolism; Plant Stems - growth & development; Plant Stems - metabolism; Seedlings - ultrastructure; SIGNALING; Transcriptional Activation - genetics; SIGNALING; Gene; Enzyme; Development; Hydrolases; Esterases; Biosynthesis; Carboxylic ester hydrolases; Galactolipase
• ISSN: 1534-5807; 1878-1551
• DOI: 10.1016/j.devcel.2007.11.010

Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A1 activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.