Sorption, solubility and cytotoxicity of novel antibacterial nanofilled dental adhesive resins

• Published in: Scientific reports Vol. 10; no. 1; p. 13503
• Main Authors: Esteban Florez, Fernando Luis, Kraemer, Hannah, Hiers, Rochelle Denise, Sacramento, Catharina Marques, Rondinone, Adam Justin, Silvério, Karina Gonzales, Khajotia, Sharukh S.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11-08-2020; Nature Publishing Group
• Subjects: 60 APPLIED LIFE SCIENCES; 692/700; 692/700/3032; 692/700/3032/3065; 692/700/3032/3065/3070; Adhesiveness; Adhesives; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - toxicity; Cell Survival - drug effects; Cytotoxicity; Dental pulp; Dental Pulp - cytology; Dental restorative materials; Humanities and Social Sciences; Humans; multidisciplinary; Nanoparticles; Nanostructures - chemistry; Resins; Resins, Synthetic - chemistry; Resins, Synthetic - toxicity; Science; Science (multidisciplinary); Solubility; Sorption; Titanium dioxide; Water - chemistry
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-70487-z

Dental adhesives hydrolyze in the mouth. This study investigated the water sorption (SOR), solubility (SOL) and cytotoxicity (CYTO) of experimental adhesives containing nitrogen-doped titanium dioxide nanoparticles (N_TiO
2
). Specimens (n = 15/group [SOR, SOL]; n = 10/group [CYTO]) of unaltered Clearfil SE Protect (CSP), OptiBond Solo Plus (OSP), Adper Scotchbond (ASB) and experimental adhesives (OSP + 25% or 30% of N_TiO
2
) were fabricated, desiccated (37 °C) and tested for SOR and SOL according to ISO Specification 4049 (2009). CYTO specimens were UV-sterilized (8 J/cm
2
) and monomer extracted in growth medium (1, 3 or 7 days). Human pulp cells were isolated and seeded (0.5 × 10
4
) for MTT assay. SOR and SOL data was analyzed using GLM and SNK (α = 0.05) and CYTO data was analyzed with Kruskal–Wallis and SNK tests (α = 0.05). SOR and SOL values ranged from 25.80 μg/mm
3
(30% N_TiO
2
) to 28.01 μg/mm
3
(OSP) and 23.88 μg/mm
3
(30% N_TiO
2
) to 25.39 μg/mm
3
(25% N_TiO
2
). CYTO results indicated that pulp cells exposed to experimental materials displayed comparable viabilities (p > 0.05) to those of OSP. Experimental materials displayed comparable SOR, SOL and CYTO values (p > 0.05) when compared to unaltered materials. N_TiO
2
incorporation have not adversely impacted SOR, SOL and CYTO properties of unaltered adhesives.