Identification of mutations that disrupt phosphorylation-dependent nuclear export of cyclin D1

• Published in: Oncogene Vol. 25; no. 47; pp. 6291 - 6303
• Main Authors: BENZENO, S, LU, F, GUO, M, BARBASH, O, ZHANG, F, HERMAN, J. G, KLEIN, P. S, RUSTGI, A, DIEHL, J. A
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 12-10-2006; Nature Publishing Group
• Subjects: Alleles; Amino Acid Substitution; Animals; Biological and medical sciences; C-Terminus; Cancer; Carcinoma - genetics; Carcinoma - metabolism; Carcinoma - pathology; Cell Line; Cell Line, Tumor - chemistry; Cell Nucleus - metabolism; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Cell Transformation, Neoplastic - genetics; Cells; Cyclin D; Cyclin D1; Cyclin D1 - genetics; Cyclin D1 - metabolism; Cyclin-Dependent Kinase 4 - metabolism; Cyclins - genetics; Cyclins - metabolism; D1 protein; DNA Mutational Analysis; DNA, Neoplasm - genetics; Enzymes; Esophageal cancer; Esophageal carcinoma; Esophageal Neoplasms - genetics; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - pathology; Esophagus; Fundamental and applied biological sciences. Psychology; Glycogen; Glycogen synthase kinase 3; Glycogen Synthase Kinase 3 - metabolism; Glycogen Synthase Kinase 3 beta; Humans; Kinases; Mice; Molecular and cellular biology; Mutants; Mutation; Mutation, Missense; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; NIH 3T3 Cells; Nuclear transport; Phosphorylation; Phosphothreonine - metabolism; Point Mutation; Protein Processing, Post-Translational; Protein Transport - genetics; Recombinant Fusion Proteins - metabolism; Sequence Deletion; Spodoptera; Threonine; Throat; Tumor cell lines; Tumorigenesis; Tumors; Phosphorylation; Enzyme; Transferases; Identification; Malignant tumor; Cyclin D1; Carcinogenesis; GSK-3β; CDK4; nuclear export; Mutation; Nucleocytoplasmic transport; Cyclin dependent kinase; Cancer
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/sj.onc.1209644

Although cyclin D1 is overexpressed in a significant number of human cancers, overexpression alone is insufficient to promote tumorigenesis. In vitro studies have revealed that inhibition of cyclin D1 nuclear export unmasks its neoplastic potential. Cyclin D1 nuclear export depends upon phosphorylation of a C-terminal residue, threonine 286, (Thr-286) which in turn promotes association with the nuclear exportin, CRM1. Mutation of Thr-286 to a non-phosphorylatable residue results in a constitutively nuclear cyclin D1 protein with significantly increased oncogenic potential. To determine whether cyclin D1 is subject to mutations that inhibit its nuclear export in human cancer, we have sequenced exon 5 of cyclin D1 in primary esophageal carcinoma samples and in cell lines derived from esophageal cancer. Our work reveals that cyclin D1 is subject to mutations in primary human cancer. The mutations identified specifically disrupt phosphorylation of cyclin D1 at Thr-286, thereby enforcing nuclear accumulation of cyclin D1. Through characterization of these mutants, we also define an acidic residue within the C-terminus of cyclin D1 that is necessary for recognition and phosphorylation of cyclin D1 by glycogen synthase kinase-3 beta. Finally, through construction of compound mutants, we demonstrate that cell transformation by the cancer-derived cyclin D1 alleles correlates with their ability to associate with and activate CDK4. Our data reveal that cyclin D1 is subject to mutations in primary human cancer that specifically disrupt phosphorylation-dependent nuclear export of cyclin D1 and suggest that such mutations contribute to the genesis and progression of neoplastic growth.