Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 111; no. 12; pp. 731 - 736
• Main Authors: Soto, Yudira, Limia, Celia Maria, González, Licet, Grá, Bienvenido, Hano, Olga Marina, Martínez, Pedro Ariel, Kourí, Vivian
• Format: Journal Article
• Language: English
• Published: Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01-12-2016; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Adenocarcinoma - virology; Adenoma - virology; Adult; colorectal cancer; Colorectal Neoplasms - virology; Cuba; DNA, Viral - analysis; Female; Genotype; human papillomavirus; Humans; Male; Middle Aged; Papillomaviridae - genetics; Papillomavirus Infections - complications; PARASITOLOGY; real-time PCR; Real-Time Polymerase Chain Reaction; TROPICAL MEDICINE; Viral Load; colorectal cancer; human papillomavirus; real-time PCR
• ISSN: 0074-0276; 1678-8060; 1678-8060
• DOI: 10.1590/0074-02760160217

The association between colorectal cancer and human papillomavirus (HPV) infection is still unproven. The aim of this study was to investigate the presence of high-risk HPV (HR-HPV) DNA in colorectal tissues from Cuban patients. A total of 63 colorectal formalin-fixed paraffin-embedded tissues were studied (24 adenocarcinoma, 18 adenoma, and 21 colorectal tissues classified as benign colitis). DNA from colorectal samples was analysed by quantitative real-time polymerase chain reaction to detect the most clinically relevant high HR-HPV types (HPV-16, -18, -31, -33, -45, -52, and -58). Associations between histologic findings and other risk factors were also analysed. Overall, HPV DNA was detected in 23.8% (15/63) of the samples studied. Viral infections were detected in 41.7% of adenocarcinoma (10/24) and 27.7% of adenoma cases (5/18). HPV DNA was not found in any of the negative cases. An association between histological diagnosis of adenocarcinoma and HPV infection was observed (odd ratio = 4.85, 95% confidence interval = 1.40-16.80, p = 0.009). The only genotypes identified were HPV 16 and 33. Viral loads were higher in adenocarcinoma, and these cases were associated with HPV 16. This study provides molecular evidence of HR-HPV infection in colorectal adenocarcinoma tissues from Cuban patients.