Helicobacter Infection in the Surfactant Protein D-Deficient Mouse

• Published in: Helicobacter (Cambridge, Mass.) Vol. 12; no. 2; pp. 112 - 123
• Main Authors: Khamri, Wafa, Worku, Mulugeta L., Anderson, Amy E., Walker, Marjorie M., Hawgood, Samuel, Reid, Kenneth B.M., Clark, Howard W., Thursz, Mark R.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-04-2007
• Subjects: Agglutination; Animals; Cells, Cultured; Dendritic Cells - microbiology; Felis; Female; Gastritis - microbiology; Gastritis - pathology; Helicobacter; Helicobacter felis; Helicobacter felis - immunology; Helicobacter felis - pathogenicity; Helicobacter Infections - metabolism; Helicobacter Infections - microbiology; Helicobacter Infections - pathology; inflammation; innate immunity; Mice; Mice, Inbred C57BL; Mice, Mutant Strains; mucosal immunity; Pulmonary Surfactant-Associated Protein D - genetics; Pulmonary Surfactant-Associated Protein D - physiology; Surfactant Protein D; T-Lymphocytes - pathology
• ISSN: 1083-4389; 1523-5378
• DOI: 10.1111/j.1523-5378.2007.00480.x

Background:  Surfactant protein D (SP‐D), a component of innate immunity, is expressed in the gastric mucosa and is up‐regulated in the presence of Helicobacter infection. SP‐D binds to Helicobacter in vitro, suggesting the involvement of SP‐D in Helicobacter‐induced immune responses. The aim of this study was to determine the role of SP‐D in gastric epithelial defense in vivo.
Methods:  Specific pathogen‐free SP‐D‐deficient mice (SP‐D−/–) and C57BL/6 wild‐type controls were challenged by gavage with different doses of Helicobacter felis, a mouse‐adapted Helicobacter strain. Mice were assessed for colonization rates and density of infection. Inflammatory responses were measured by neutrophil counting and T‐cell responses by proliferation assays on spleen cells stimulated with H. felis sonicate. The in vitro effect of SP‐D on Helicobacter uptake by monocyte‐derived dendritic cells was assessed by confocal microscopy and FACS analyses.
Results:  SP‐D−/– mice were more susceptible to low‐dose infectious challenge than C57BL/6 controls (p = .02). The density of colonization was higher in the SP‐D−/– infected mice. Neutrophil infiltrates were lower in the SP‐D−/– mice, particularly in the acid‐secreting regions of the stomach. T‐cell proliferative responses to Helicobacter antigen were reduced in SP‐D−/– mice (p = .001) after 12 weeks infection. In vitro uptake of Helicobacter by dendritic cells was significantly enhanced in the presence of SP‐D (p = .001).
Conclusion:  In the absence of SP‐D, Helicobacter uptake by dendritic cells is impaired. This provides an explanation for the diminished inflammation and immune responses in the SP‐D−/– mice.