A serologic study of canine herpes virus-1 infection in the Norwegian adult dog population

• Published in: Theriogenology Vol. 78; no. 1; pp. 153 - 158
• Main Authors: Krogenæs, A, Rootwelt, V, Larsen, S, Sjøberg, E.K, Akselsen, B, Skår, T.M, Myhre, S.S, Renström, L.H.M, Klingeborn, B, Lund, A
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-07-2012
• Subjects: adults; Age Factors; Animals; antibodies; Antibodies, Viral - analysis; Antibodies, Viral - blood; blood; Canine herpes virus-1; Cross-Sectional Studies; Dog; Dog Diseases - blood; Dog Diseases - epidemiology; Dog Diseases - immunology; Dogs; Female; Herpesviridae Infections - blood; Herpesviridae Infections - epidemiology; Herpesviridae Infections - veterinary; Herpesviridae Infections - virology; Herpesvirus 1, Canid - immunology; Herpesvirus 1, Canid - isolation & purification; Male; Norway; Norway - epidemiology; Population; puppies; Sero-epidemiology; Seroepidemiologic Studies; Serologic Tests - statistics & numerical data; Serologic Tests - veterinary; serological surveys; seroprevalence; Norway; Canine herpes virus-1; Norway; Dog; Sero-epidemiology
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2012.01.031

Canine herpes virus-1 (CHV1) causes a fatal hemorrhagic disease in neonatal puppies and is associated with reproductive problems in female dogs. This serologic study was conducted to assess the seroprevalence of CHV1 infection in Norway. Blood samples were collected from clinically healthy dogs (n = 436) one yr of age and older of both genders, supplied by four small animal clinics (A, B, C and D) in different parts of the country. The immunoperoxidase monolayer assay was used for testing of CHV1 antibodies. Serum titers were recorded as the reciprocal value of the highest dilution producing specific cell staining. Titers equal to or above 80 were considered positive for exposure to CHV1. In total, 80.0% of the dogs had titers ≥80 and were classified as positive. Mean age for seronegative dogs was 4.7 yrs (95% CI 4.1–5.4) and for seropositive dogs 5.0 yrs (95% CI 4.7–5.4). Of the dogs, 32.8% displayed a weakly positive titer of 80, whereas 41.5 and 5.7% fell into the moderately (titer 160 and 320) and strongly (titer ≥640) positive categories, respectively. No association was demonstrated when comparing CHV1 antibody titers to gender or reproductive parameters like previous matings, pregnancies, births or number of puppies born. Age, visit in foreign countries and clinic explained together 78% of the variation in antibody titer categories. The percentage of positive samples differed significantly between the four clinics (A 98%, B 58.5%, C 74.6%, D 89.5%). A reasonable explanation for this finding has not been established. No information about an ongoing outbreak of CHV1 infection was available. In conclusion, this study strongly indicates that CHV1 infection is endemic in the dog population of Norway. There are significant differences in seroprevalence between geographic regions in the country.