Brain Glutamate Decarboxylase Cloned in λgt-11: Fusion Protein Produces γ-Aminobutyric Acid

Glutamate decarboxylase (GAD; E.C. 4.1.1.15) converts glutamate to γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the vertebrate central nervous system. This report describes the isolation of a GAD complementary DNA clone by immunological screening of a λgt-11 brain complementa...

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Bibliographic Details
Published in:	Science (American Association for the Advancement of Science) Vol. 232; no. 4754; pp. 1138 - 1140
Main Authors:	Kaufman, Daniel L., McGinnis, James F., Krieger, Neil R., Tobin, Allan J.
Format:	Journal Article
Language:	English
Published:	Washington, DC The American Association for the Advancement of Science 30-05-1986 American Association for the Advancement of Science
Subjects:	Amino acids Animals Antibodies Antiserum Bacteria Biological and medical sciences Biotechnology Brain Brain - enzymology Cell physiology Cloning, Molecular Complementary DNA DNA - genetics Epitopes Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - biosynthesis Genetic engineering Genetic screening Genetic technics Glutamate Decarboxylase - biosynthesis Glutamate Decarboxylase - genetics Glutamate Decarboxylase - metabolism Humans Immunology Libraries Methods. Procedures. Technologies Mice Molecular and cellular biology Molecular cloning Neurotransmission phage lambda Rats Recombinant Proteins - biosynthesis Recombinant Proteins - metabolism Bactériophage lambda Enzyme Brain (Vertebrata) Glutamate decarboxylase Gene library Immunological method Virus Lambda phage group Enzymatic activity Complementary DNA Styloviridae Neurotransmitter Bactériophage Molecular cloning
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