Structural and Functional Insights into the Evolution and Stress Adaptation of Type II Chaperonins

• Published in: Structure (London) Vol. 24; no. 3; pp. 364 - 374
• Main Authors: Chaston, Jessica J., Smits, Callum, Aragão, David, Wong, Andrew S.W., Ahsan, Bilal, Sandin, Sara, Molugu, Sudheer K., Molugu, Sanjay K., Bernal, Ricardo A., Stock, Daniela, Stewart, Alastair G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01-03-2016
• Subjects: Adaptation; Archaeal Proteins - chemistry; Archaeal Proteins - metabolism; Bacteria; Biological evolution; Crystallography, X-Ray; Evolution; Evolution, Molecular; Group II Chaperonins - chemistry; Group II Chaperonins - metabolism; Heat stress; Kinetics; Microscopy, Electron; Models, Molecular; Phylogeny; Protein Multimerization; Protein Structure, Secondary; Proteins; Structural analysis; Substrate Specificity; Substrates; Sulfolobus solfataricus - metabolism; Temperature
• ISSN: 0969-2126; 1878-4186
• DOI: 10.1016/j.str.2015.12.016

Chaperonins are essential biological complexes assisting protein folding in all kingdoms of life. Whereas homooligomeric bacterial GroEL binds hydrophobic substrates non-specifically, the heterooligomeric eukaryotic CCT binds specifically to distinct classes of substrates. Sulfolobales, which survive in a wide range of temperatures, have evolved three different chaperonin subunits (α, β, γ) that form three distinct complexes tailored for different substrate classes at cold, normal, and elevated temperatures. The larger octadecameric β complexes cater for substrates under heat stress, whereas smaller hexadecameric αβ complexes prevail under normal conditions. The cold-shock complex contains all three subunits, consistent with greater substrate specificity. Structural analysis using crystallography and electron microscopy reveals the geometry of these complexes and shows a novel arrangement of the α and β subunits in the hexadecamer enabling incorporation of the γ subunit.
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•Identification of three distinct TF55 chaperonin complexes in Sulfolobus solfataricus•Structural analyses of these suggests different kinetics and substrate specificities•Geometry of complexes sheds new light on chaperonin assembly and evolution
Thermophiles survive under wide ranges of extreme conditions. Sulfolobales have evolved a modular system of chaperonins that rescue different classes of substrate proteins under different conditions. Structural analyses of three different complexes sheds new light on substrate specificity and dynamics as well as on the assembly and evolution of chaperonins.