Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates

• Published in: Journal of biotechnology Vol. 121; no. 4; pp. 482 - 485
• Main Authors: te Biesebeke, R., Boussier, A., van Biezen, N., van den Hondel, C.A.M.J.J., Punt, P.J.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 24-02-2006; Amsterdam Elsevier; New York, NY
• Subjects: alpha-amylase; alpha-N-arabinofuranosidase; Aspergillus oryzae; Aspergillus oryzae - growth & development; Biological and medical sciences; Biotechnology; Cereal substrates; Edible Grain - microbiology; enzymes; extracellular enzymes; Filamentous; Fundamental and applied biological sciences. Psychology; fungal proteins; Fungal Proteins - analysis; Fungal Proteins - secretion; peptides; protein secretion; solid state fermentation; state fermentation; wheat; xylanases; Aspergillus oryzae; Filamentous; Cereal substrates; Fungi; Substrate; Growth; Secretory protein; Identification; Fungi Imperfecti; Thallophyta
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2005.08.028

Filamentous growth of
Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of
A. oryzae grown on wheat-based media were analysed using N-terminal sequence analysis. In a submerged wheat-based growth medium of
A. oryzae, besides α-amylase, also an arabinosidase and xylanase were abundantly produced. In the extracts of
A. oryzae grown on wheat-based solid substrate besides α-amylase and chitinase, two new proteins of 16 and 27
kDa were identified. These hypothetical proteins showed only close homologies to filamentous fungal proteins.