Complete genome sequence of a novel endornavirus isolated from hot pepper

• Published in: Archives of virology Vol. 160; no. 12; pp. 3153 - 3156
• Main Authors: Lim, Seungmo, Kim, Kil Hyun, Zhao, Fumei, Yoo, Ran Hee, Igori, Davaajargal, Lee, Su-Heon, Moon, Jae Sun
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-12-2015; Springer Nature B.V
• Subjects: Annotated Sequence Record; Base Sequence; Biomedical and Life Sciences; Biomedicine; Capsicum - virology; Capsicum annuum; Cloning; cultivars; Endornavirus; genome; Genome, Viral; Genomes; hot peppers; Infectious Diseases; Medical Microbiology; methyltransferases; Molecular Sequence Data; new species; Open Reading Frames; pepper; Phylogeny; Plant Diseases - virology; polyproteins; Research centers; RNA helicases; RNA polymerase; RNA Viruses - classification; RNA Viruses - genetics; RNA Viruses - isolation & purification; RNA-directed RNA polymerase; sequence homology; sweet peppers; Virology; Viruses; United States; United States > US; South Korea; Large Open Reading Frame; High Nucleotide Sequence Identity; Query Cover; Capsicum Annuum; High Amino Acid Sequence Identity
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-015-2616-7

The complete genome of a putative new endornavirus infecting hot peppers (Capsicum annuum) was determined to be 14,729 nt in size, including 12 cytosines at the 3′ end. The hot pepper-infecting virus has the highest nucleotide sequence similarity (94 % query cover and 72 % identity) to bell pepper endornavirus (BPEV) isolated from the cultivar Yolo Wonder in the USA (GenBank accession no. JN019858). The putative single, large open reading frame encodes a 4,884-amino-acid-long polyprotein that contains four putative functional domains: a viral methyltransferase, a viral RNA helicase, a glycosyltransferase, and an RNA-dependent RNA polymerase. A phylogenetic tree based on whole polyprotein sequences confirmed the close evolutionary relationship of the studied endornavirus to BPEV. The hot pepper-infecting virus also has a nick at nt position 975. Taken together, these results suggest that this virus belongs to a new species in the genus Endornavirus (family Endornaviridae), for which the name hot pepper endornavirus (HPEV) is proposed.