Platonia insignis Mart., a Brazilian Amazonian Plant: The Stem Barks Extract and Its Main Constituent Lupeol Exert Antileishmanial Effects Involving Macrophages Activation

• Published in: Evidence-based complementary and alternative medicine Vol. 2017; no. 2017; pp. 1 - 12
• Main Authors: Arcanjo, Daniel Dias Rufino, Carvalho, Fernando Aécio de Amorim, Chaves, Mariana H., Freitas, Sâmya Danielle Lima, Costa, Isabella Cristhina Gonçalves, Sobrinho-Júnior, Enoque Pereira Costa, Oliveira, Luciano Gomes De Castro, Brito, Lucas Moreira, Alves, Michel Muálem de Moraes, Souza, Adriana Cunha, Rodrigues, Klinger Antonio da Franca
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2017; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Amastigotes; Apoptosis; Bark; Biological properties; Cancer; Cell activation; Chemistry; Chemotherapy; Chromatography; Cytotoxicity; Ethanol; Herbal medicine; Immunomodulation; Leishmania; Leishmaniasis; Macrophages; Medical research; Parasitic diseases; Phagocytes; Plant extracts; Risk factors; Brazil
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2017/3126458

Platonia insignis Mart., popularly known as “bacurizeiro,” is used in traditional medical practices based on its diverse biological properties. This study was aimed at evaluating the antileishmanial effects of the ethanol extract (EtOH-Ext), hexane fraction (Hex-F), and its main isolated Lupeol obtained from stem barks of P. insignis against Leishmania (Leishmania) amazonensis, as well as their cytotoxicity and possible mechanisms of action. The EtOH-Ext, Hex-F, and Lupeol inhibited the growth of L. amazonensis promastigote forms at IC50 of 174.24, 45.23, and 39.06 µg/mL, respectively, as well as L. amazonensis axenic amastigote forms at IC50 of 40.58, 35.87, and 44.10 µg/mL, respectively. The mean cytotoxic concentrations for macrophages (CC50) were higher than those for amastigotes (341.95, 71.65, and 144.0 µg/mL, resp.), indicating a selective cytotoxicity towards the parasite rather than the macrophages. Interestingly, all treatments promoted antileishmanial effect against macrophage-internalized amastigotes at concentrations lower than CC50. Furthermore, increases of lysosomal volume of macrophages treated with EtOH-Ext, Hex-F, and Lupeol were observed. On the other hand, only Lupeol stimulated increase of phagocytic capability of macrophages, suggesting this compound might be characterized as the biomarker for the antileishmanial effect of P. insignis stem bark, as well as the involvement of immunomodulatory mechanisms in this effect.