Effects of Diacetyl Flavoring Exposure in Mice Metabolism

• Published in: BioMed research international Vol. 2018; no. 2018; pp. 1 - 11
• Main Authors: Bechara, J. Etelvino H., Assunção, Nilson Antonio, Penatti, Carlos Alberto Avellaneda, Cavalcanti, Fernanda de Barros Correia, Silva, Heron Dominguez Torres da, Furtado, Danielle Zildeana Sousa, Neto, Giuseppe Bruno, Balbino, Aleksandro Martins, Silva, Juciara da Costa, Jedlicka, Letícia Dias Lima, Heijden, Karin Marie van der
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2018; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Amino acids; Animals; Biomedical research; Chromatography, High Pressure Liquid; Chromatography, Liquid; Colorimetry; Diacetyl; Diacetyl - pharmacology; Drinking water; Endothelium; Enzymes; Exposure; Female; Females; Flavor; Flavoring Agents - pharmacology; Flavoring essences; Food products; Gender aspects; Gene expression; Hormones; Hormones, Sex; Liquid chromatography; Male; Mass spectrometry; Mass spectroscopy; Metabolism; Metabolites; Metabolomics; Mice; Occupational health and safety; Peroxynitrite; Physiological aspects; Quadrupoles; Rodents; Scientific imaging; Serotonin; Sex; Sex differences; Sex hormones; Superoxide; Superoxide dismutase; Tandem Mass Spectrometry; Urea; Brazil
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2018/9875319

Diacetyl is a flavoring that imparts a buttery flavor to foods, but the use or exposure to diacetyl has been related to some diseases. We investigated the effect of oral intake of diacetyl in male and female C57/Bl mice. We performed a target metabolomics assay using ultraperformance liquid chromatography paired with triple quadrupole mass spectrometry (UPLC-MS/MS) for the determination and quantification of plasmatic metabolites. We observed alterations in metabolites present in the urea and tricarboxylic acid (TCA) cycles. Peroxynitrite plasmatic levels were evaluated by a colorimetric method, final activity of superoxide dismutase (SOD) was evaluated by an enzymatic method, and mouse behavior was evaluated. Majority of the assay showed differences between control and treatment groups, as well as between genders. This may indicate the involvement of sex hormones in the regulation of a normal metabolic profile, and the implication of sex differences in metabolite disease response.