A next-generation sequencing method for gene doping detection that distinguishes low levels of plasmid DNA against a background of genomic DNA

Gene doping confers health risks for athletes and is a threat to fair competition in sports. Therefore the anti-doping community has given attention on its detection. Previously published polymerase chain reaction-based methodologies for gene doping detection are targeting exon–exon junctions in the...

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Bibliographic Details
Published in:	Gene therapy Vol. 26; no. 7-8; pp. 338 - 346
Main Authors:	de Boer, Eddy N., van der Wouden, Petra E., Johansson, Lennart F., van Diemen, Cleo C., Haisma, Hidde J.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01-08-2019 Nature Publishing Group
Subjects:	42 42/44 45 45/23 631/1647/1513 631/1647/2300/1514 631/61/2300/1514 Biomedical and Life Sciences Biomedicine Cell Biology Deoxyribonucleic acid DNA Doping in Sports - methods Erythropoietin - genetics Erythropoietin - metabolism Exons Gene Expression Gene Therapy Genes Genetic engineering Genetic Testing - methods Genetic Testing - standards Genome, Human Genomics High-Throughput Nucleotide Sequencing - methods High-Throughput Nucleotide Sequencing - standards Human Genetics Humans Insulin-like growth factor I Insulin-like growth factor II Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism Nanotechnology Next-generation sequencing Nucleotide sequence Plasmids - genetics Plasmids - metabolism Polymerase chain reaction Reproducibility of Results Sensitivity and Specificity Sequence Analysis, DNA - methods Sequence Analysis, DNA - standards Transgenes
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