Rapid identification of Streptococcus pyogenes with PCR primers from a putative transcriptional regulator gene

Streptococcus pyogenes (GAS) is a common bacterial pathogen that has emerged as an increasingly important health concern in many parts of the world. Although GAS may appear harmless in healthy individuals, the ability of this bacterium to take advantage of a weakened or compromised host defense syst...

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Published in:	Research in microbiology Vol. 156; no. 4; pp. 564 - 567
Main Authors:	Liu, Dongyou, Hollingshead, Susan, Swiatlo, Edwin, Lawrence, Mark L., Austin, Frank W.
Format:	Journal Article
Language:	English
Published:	Paris Elsevier SAS 01-05-2005 Elsevier
Subjects:	Bacteriology Biological and medical sciences DNA Primers - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genes, Regulator Humans Identification Microbiology Miscellaneous PCR Polymerase Chain Reaction - methods Species Specificity Streptococcus pyogenes Streptococcus pyogenes - genetics Streptococcus pyogenes - pathogenicity Transcription, Genetic Transcriptional regulator gene Virulence - genetics Streptococcus pyogenes Identification PCR Transcriptional regulator gene Polymerase chain reaction Streptococcaceae Microbiology Regulator gene Bacteria Micrococcales
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Abstract	Streptococcus pyogenes (GAS) is a common bacterial pathogen that has emerged as an increasingly important health concern in many parts of the world. Although GAS may appear harmless in healthy individuals, the ability of this bacterium to take advantage of a weakened or compromised host defense system is extraordinary. Following the recent publication of the genome sequences of several S. pyogenes strains, we undertook an investigation of a specialized gene group in GAS that encodes transcriptional regulators. By screening S. pyogenes transcriptional regulator genes from the complete genome of M1 strain SF370 against other DNA sequences at GenBank by BLAST searches, we identified a gene (i.e., Spy1258) that is uniquely present in the bacterium. Application of PCR primers (spy1258F and spy1258R) derived from this gene facilitated amplification of a 407-bp DNA fragment from S. pyogenes only, but not from other species of the genus Streptococcus and common bacteria. Apart from offering an additional target for specific confirmation of GAS, further analysis of the putative transcriptional regulator gene Spy1258 and its related protein product may lead to new insights into the molecular mechanisms of S. pyogenes maintenance and pathogenicity.
AbstractList	Streptococcus pyogenes (GAS) is a common bacterial pathogen that has emerged as an increasingly important health concern in many parts of the world. Although GAS may appear harmless in healthy individuals, the ability of this bacterium to take advantage of a weakened or compromised host defense system is extraordinary. Following the recent publication of the genome sequences of several S. pyogenes strains, we undertook an investigation of a specialized gene group in GAS that encodes transcriptional regulators. By screening S. pyogenes transcriptional regulator genes from the complete genome of M1 strain SF370 against other DNA sequences at GenBank by BLAST searches, we identified a gene (i.e., Spy1258) that is uniquely present in the bacterium. Application of PCR primers (spy1258F and spy1258R) derived from this gene facilitated amplification of a 407-bp DNA fragment from S. pyogenes only, but not from other species of the genus Streptococcus and common bacteria. Apart from offering an additional target for specific confirmation of GAS, further analysis of the putative transcriptional regulator gene Spy1258 and its related protein product may lead to new insights into the molecular mechanisms of S. pyogenes maintenance and pathogenicity. Streptococcus pyogenes (GAS) is a common bacterial pathogen that has emerged as an increasingly important health concern in many parts of the world. Although GAS may appear harmless in healthy individuals, the ability of this bacterium to take advantage of a weakened or compromised host defense system is extraordinary. Following the recent publication of the genome sequences of several S. pyogenes strains, we undertook an investigation of a specialized gene group in GAS that encodes transcriptional regulators. By screening S. pyogenes transcriptional regulator genes from the complete genome of M1 strain SF370 against other DNA sequences at GenBank by BLAST searches, we identified a gene (i.e., Spy1258) that is uniquely present in the bacterium. Application of PCR primers (spy1258F and spy1258R) derived from this gene facilitated amplification of a 407-bp DNA fragment from S. pyogenes only, but not from other species of the genus Streptococcus and common bacteria. Apart from offering an additional target for specific confirmation of GAS, further analysis of the putative transcriptional regulator gene Spy1258 and its related protein product may lead to new insights into the molecular mechanisms of S. pyogenes maintenance and pathogenicity.
Author	Swiatlo, Edwin Hollingshead, Susan Lawrence, Mark L. Liu, Dongyou Austin, Frank W.
Author_xml	– sequence: 1 givenname: Dongyou surname: Liu fullname: Liu, Dongyou email: liu@cvm.msstate.edu organization: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA – sequence: 2 givenname: Susan surname: Hollingshead fullname: Hollingshead, Susan organization: Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA – sequence: 3 givenname: Edwin surname: Swiatlo fullname: Swiatlo, Edwin organization: Veterans Affairs Medical Center, Jackson, MS 39216, USA – sequence: 4 givenname: Mark L. surname: Lawrence fullname: Lawrence, Mark L. organization: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA – sequence: 5 givenname: Frank W. surname: Austin fullname: Austin, Frank W. organization: Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
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Cites_doi	10.1128/JCM.40.11.4207-4210.2002 10.1542/peds.113.4.924 10.1007/s004310051325 10.1128/CMR.13.3.470 10.1016/S0378-1097(03)00378-1 10.1101/gr.1096703 10.1016/j.ijfoodmicro.2003.07.004 10.1099/jmm.0.05358-0 10.1111/j.1472-765X.2004.01629.x 10.1097/00006454-200210000-00007 10.1111/j.1365-2958.1991.tb02158.x 10.1073/pnas.152298499 10.1073/pnas.062526099 10.1073/pnas.071559398 10.1016/j.mimet.2004.04.008 10.1016/0140-6736(90)92777-F
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References	Cunningham (bib005) 2000; 13 Liu, Ainsworth, Austin, Lawrence (bib009) 2003; 52 Chapin, Blake, Wilson (bib004) 2002; 40 Smoot, Barbian, Van Gompel, Smoot, Chaussee, Sylva, Sturdevant, Ricklefs, Porcella, Parkins, Beres, Campbell, Smith, Zhang, Kapur, Daly, Veasy, Musser (bib017) 2002; 99 Gieseker, Mackenzie, Roe, Todd (bib006) 2002; 21 Liu, Ainsworth, Austin, Lawrence (bib010) 2004; 91 Musser, Krause (bib014) 1998 Liu, Lawrence, Austin (bib011) 2004; 58 Armengol, Schlager, Hendley (bib001) 2004; 113 Beres, Sylva, Barbian, Lei, Hoff, Mammarella, Liu, Smoot, Porcella, Parkins, McCormick, Leung, Schlievert, Musser (bib002) 2002; 99 Schwartz, Facklam, Breiman (bib016) 1990; 336 Mengaud, Dramsi, Gouin, Vazquez-Boland, Milon, Cossart (bib013) 1991; 5 Nakagawa, Kurokawa, Yamashita, Nakata, Tomiyasu, Okahashi, Kawabata, Yamazaki, Shiba, Yasunaga, Hayashi, Hattori, Hamada (bib015) 2003; 13 Berner, Herdeg, Gordjani, Brandis (bib003) 2000; 159 Ferretti, McShan, Adjic, Savic, Savic, Lyon, Primeaux, Sezate, Surorov, Kenton, Lai, Lin, Qian, Jia, Najar, Ren, Zhu, Song, White, Yuan, Clifton, Roe, McLaughlin (bib007) 2001; 98 Liu, Ainsworth, Austin, Lawrence (bib008) 2003; 223 Liu, Lawrence, Austin (bib012) 2005; 40 Nakagawa (10.1016/j.resmic.2005.01.010_bib015) 2003; 13 Liu (10.1016/j.resmic.2005.01.010_bib011) 2004; 58 Musser (10.1016/j.resmic.2005.01.010_bib014) 1998 Chapin (10.1016/j.resmic.2005.01.010_bib004) 2002; 40 Armengol (10.1016/j.resmic.2005.01.010_bib001) 2004; 113 Beres (10.1016/j.resmic.2005.01.010_bib002) 2002; 99 Gieseker (10.1016/j.resmic.2005.01.010_bib006) 2002; 21 Liu (10.1016/j.resmic.2005.01.010_bib008) 2003; 223 Cunningham (10.1016/j.resmic.2005.01.010_bib005) 2000; 13 Schwartz (10.1016/j.resmic.2005.01.010_bib016) 1990; 336 Smoot (10.1016/j.resmic.2005.01.010_bib017) 2002; 99 Ferretti (10.1016/j.resmic.2005.01.010_bib007) 2001; 98 Berner (10.1016/j.resmic.2005.01.010_bib003) 2000; 159 Liu (10.1016/j.resmic.2005.01.010_bib009) 2003; 52 Liu (10.1016/j.resmic.2005.01.010_bib010) 2004; 91 Liu (10.1016/j.resmic.2005.01.010_bib012) 2005; 40 Mengaud (10.1016/j.resmic.2005.01.010_bib013) 1991; 5
References_xml	– volume: 40 start-page: 4207 year: 2002 end-page: 4210 ident: bib004 article-title: Performance characteristics and utilization of rapid antigen test, DNA probe, and culture for detection of group A streptococci in an acute care clinic publication-title: J. Clin. Microbiol. contributor: fullname: Wilson – volume: 40 start-page: 69 year: 2005 end-page: 73 ident: bib012 article-title: Evaluation of PCR primers from putative transcriptional regulator genes for identification of publication-title: Lett. Appl. Microbiol. contributor: fullname: Austin – volume: 13 start-page: 470 year: 2000 end-page: 511 ident: bib005 article-title: Pathogenesis of group A streptococcal infections publication-title: Clin. Microbiol. Rev. contributor: fullname: Cunningham – volume: 223 start-page: 205 year: 2003 end-page: 210 ident: bib008 article-title: Identification of publication-title: FEMS Microbiol. Lett. contributor: fullname: Lawrence – volume: 58 start-page: 263 year: 2004 end-page: 267 ident: bib011 article-title: Specific PCR identification of publication-title: J. Microbiol. Methods contributor: fullname: Austin – volume: 13 start-page: 1042 year: 2003 end-page: 1055 ident: bib015 article-title: Genome sequence of an M3 strain of S publication-title: Genome Res. contributor: fullname: Hamada – volume: 336 start-page: 1167 year: 1990 end-page: 1171 ident: bib016 article-title: Changing epidemiology of group A streptococcal infections in the USA publication-title: Lancet contributor: fullname: Breiman – start-page: 185 year: 1998 end-page: 218 ident: bib014 article-title: The revival of group A streptococcal diseases, with a commentary on staphylococcal toxic shock syndrome publication-title: Emerging Infections contributor: fullname: Krause – volume: 99 start-page: 10078 year: 2002 end-page: 10083 ident: bib002 article-title: Genome sequence of a serotype M3 strain of group A publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Musser – volume: 5 start-page: 2273 year: 1991 end-page: 2283 ident: bib013 article-title: Pleiotropic control of publication-title: Mol. Microbiol. contributor: fullname: Cossart – volume: 52 start-page: 1065 year: 2003 end-page: 1070 ident: bib009 article-title: Characterization of virulent and avirulent publication-title: J. Med. Microbiol. contributor: fullname: Lawrence – volume: 91 start-page: 297 year: 2004 end-page: 304 ident: bib010 article-title: Use of PCR primers derived from a putative transcriptional regulator gene for species-specific identification of publication-title: Int. J. Food Microbiol. contributor: fullname: Lawrence – volume: 99 start-page: 4668 year: 2002 end-page: 4673 ident: bib017 article-title: Genome sequence and comparative microarray analysis of serotype M18 group A publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Musser – volume: 113 start-page: 924 year: 2004 end-page: 926 ident: bib001 article-title: Sensitivity of a rapid antigen detection test for group A streptococci in a private pediatric office setting: answering the Red Book's request for validation publication-title: Pediatrics contributor: fullname: Hendley – volume: 98 start-page: 4658 year: 2001 end-page: 4663 ident: bib007 article-title: Complete genome sequence of an M1 strain of publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: McLaughlin – volume: 159 start-page: 527 year: 2000 end-page: 529 ident: bib003 article-title: publication-title: Eur. J. Pediatr. contributor: fullname: Brandis – volume: 21 start-page: 922 year: 2002 end-page: 927 ident: bib006 article-title: Comparison of two rapid publication-title: Pediatr. Infect. Dis. J. contributor: fullname: Todd – volume: 40 start-page: 4207 year: 2002 ident: 10.1016/j.resmic.2005.01.010_bib004 article-title: Performance characteristics and utilization of rapid antigen test, DNA probe, and culture for detection of group A streptococci in an acute care clinic publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.40.11.4207-4210.2002 contributor: fullname: Chapin – volume: 113 start-page: 924 year: 2004 ident: 10.1016/j.resmic.2005.01.010_bib001 article-title: Sensitivity of a rapid antigen detection test for group A streptococci in a private pediatric office setting: answering the Red Book's request for validation publication-title: Pediatrics doi: 10.1542/peds.113.4.924 contributor: fullname: Armengol – volume: 159 start-page: 527 year: 2000 ident: 10.1016/j.resmic.2005.01.010_bib003 article-title: Streptococcus pyogenes meningitis: Report of a case and review of the literature publication-title: Eur. J. Pediatr. doi: 10.1007/s004310051325 contributor: fullname: Berner – volume: 13 start-page: 470 year: 2000 ident: 10.1016/j.resmic.2005.01.010_bib005 article-title: Pathogenesis of group A streptococcal infections publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.13.3.470 contributor: fullname: Cunningham – volume: 223 start-page: 205 year: 2003 ident: 10.1016/j.resmic.2005.01.010_bib008 article-title: Identification of Listeria innocua by PCR targeting a putative transcriptional regulator gene publication-title: FEMS Microbiol. Lett. doi: 10.1016/S0378-1097(03)00378-1 contributor: fullname: Liu – volume: 13 start-page: 1042 year: 2003 ident: 10.1016/j.resmic.2005.01.010_bib015 article-title: Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution publication-title: Genome Res. doi: 10.1101/gr.1096703 contributor: fullname: Nakagawa – volume: 91 start-page: 297 year: 2004 ident: 10.1016/j.resmic.2005.01.010_bib010 article-title: Use of PCR primers derived from a putative transcriptional regulator gene for species-specific identification of Listeria monocytogenes publication-title: Int. J. Food Microbiol. doi: 10.1016/j.ijfoodmicro.2003.07.004 contributor: fullname: Liu – volume: 52 start-page: 1065 year: 2003 ident: 10.1016/j.resmic.2005.01.010_bib009 article-title: Characterization of virulent and avirulent Listeria monocytogenes strains by PCR amplification of putative transcriptional regulator and internalin genes publication-title: J. Med. Microbiol. doi: 10.1099/jmm.0.05358-0 contributor: fullname: Liu – volume: 40 start-page: 69 year: 2005 ident: 10.1016/j.resmic.2005.01.010_bib012 article-title: Evaluation of PCR primers from putative transcriptional regulator genes for identification of Staphylococcus aureus publication-title: Lett. Appl. Microbiol. doi: 10.1111/j.1472-765X.2004.01629.x contributor: fullname: Liu – volume: 21 start-page: 922 year: 2002 ident: 10.1016/j.resmic.2005.01.010_bib006 article-title: Comparison of two rapid Streptococcus pyogenes diagnostic tests with a rigorous culture standard publication-title: Pediatr. Infect. Dis. J. doi: 10.1097/00006454-200210000-00007 contributor: fullname: Gieseker – volume: 5 start-page: 2273 year: 1991 ident: 10.1016/j.resmic.2005.01.010_bib013 article-title: Pleiotropic control of Listeria monocytogenes virulence factors by a gene that is autoregulated publication-title: Mol. Microbiol. doi: 10.1111/j.1365-2958.1991.tb02158.x contributor: fullname: Mengaud – volume: 99 start-page: 10078 year: 2002 ident: 10.1016/j.resmic.2005.01.010_bib002 article-title: Genome sequence of a serotype M3 strain of group A Streptococcus: Phage-encoded toxins, the high-virulence phenotype, and clone emergence publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.152298499 contributor: fullname: Beres – start-page: 185 year: 1998 ident: 10.1016/j.resmic.2005.01.010_bib014 article-title: The revival of group A streptococcal diseases, with a commentary on staphylococcal toxic shock syndrome contributor: fullname: Musser – volume: 99 start-page: 4668 year: 2002 ident: 10.1016/j.resmic.2005.01.010_bib017 article-title: Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.062526099 contributor: fullname: Smoot – volume: 98 start-page: 4658 year: 2001 ident: 10.1016/j.resmic.2005.01.010_bib007 article-title: Complete genome sequence of an M1 strain of Streptococcus pyogenes publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.071559398 contributor: fullname: Ferretti – volume: 58 start-page: 263 year: 2004 ident: 10.1016/j.resmic.2005.01.010_bib011 article-title: Specific PCR identification of Pasteurella multocida based on putative transcriptional regulator genes publication-title: J. Microbiol. Methods doi: 10.1016/j.mimet.2004.04.008 contributor: fullname: Liu – volume: 336 start-page: 1167 year: 1990 ident: 10.1016/j.resmic.2005.01.010_bib016 article-title: Changing epidemiology of group A streptococcal infections in the USA publication-title: Lancet doi: 10.1016/0140-6736(90)92777-F contributor: fullname: Schwartz
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SubjectTerms	Bacteriology Biological and medical sciences DNA Primers - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genes, Regulator Humans Identification Microbiology Miscellaneous PCR Polymerase Chain Reaction - methods Species Specificity Streptococcus pyogenes Streptococcus pyogenes - genetics Streptococcus pyogenes - pathogenicity Transcription, Genetic Transcriptional regulator gene Virulence - genetics
Title	Rapid identification of Streptococcus pyogenes with PCR primers from a putative transcriptional regulator gene
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