Homogeneous, bioluminescent protease assays: caspase-3 as a model

Using caspase-3 as a model, the authors have developed a strategy for highly sensitive, homogeneous protease assays suitable for high-throughput, automated applications. The assay uses peptide-conjugated aminoluciferin as the protease substrate and a firefly luciferase that has been molecularly evol...

Full description

Bibliographic Details

Published in:	Journal of biomolecular screening Vol. 10; no. 2; p. 137
Main Authors:	O'Brien, Martha A, Daily, William J, Hesselberth, P Eric, Moravec, Richard A, Scurria, Michael A, Klaubert, Dieter H, Bulleit, Robert F, Wood, Keith V
Format:	Journal Article
Language:	English
Published:	United States 01-03-2005
Subjects:	Caspase 3 Caspases - metabolism Cell Line, Tumor Dimethyl Sulfoxide Fluorescence Humans Inhibitory Concentration 50 Luminescent Measurements - methods Models, Biological Molecular Structure Sensitivity and Specificity
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!