Improved growth of Flavobacterium psychrophilum using a new culture medium

• Published in: Aquaculture Vol. 238; no. 1; pp. 75 - 82
• Main Authors: Cepeda, Catalina, Garcı́a-Márquez, Sonia, Santos, Ysabel
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-09-2004; Elsevier Science; Elsevier Sequoia S.A
• Subjects: Anguilla anguilla; Animal aquaculture; animal pathogenic bacteria; Animal productions; Aquaculture; bacterial infections; Biological and medical sciences; Cold-water disease (CWD); culture media; Culture medium; Cyprinus carpio; Disease; Fish; fish culture; fish diseases; Flavobacterium psychrophilum; Fundamental and applied biological sciences. Psychology; General aspects; Growth; Oncorhynchus kisutch; Oncorhynchus mykiss; Rainbow trout fry syndrome (RTFS); salmon; Yeast; Culture medium; Flavobacterium psychrophilum; Rainbow trout fry syndrome (RTFS); Cold-water disease (CWD); Growth; Disease; Fry; Oncorhynchus mykiss; Syndrome; Vertebrata; Cold water; Pisces; Developmental stage; Aquaculture
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2004.05.013

The comparative study of different media used for cultivation of
Flavobacterium psychrophilum showed that tryptone–yeast extract–salts supplemented with glucose, named FLP medium, was the most appropriate for the growth of this fish pathogen. By means of the endpoint dilution method, it was found that the bacterium grew more efficiently on the improved FLP medium than on the other four solid media assayed. This medium was also the most effective for the recovery of the pathogen from tissues of diseased fish. Culture of
F. psychrophilum in FLP broth was also more rapid and provided high cell yields. In addition, FLP is a low-cost and easy-to-prepare medium.