Regulation of atrial natriuretic factor receptors by angiotensin II in rat vascular smooth muscle cells

• Published in: The Journal of biological chemistry Vol. 263; no. 26; pp. 13199 - 13202
• Main Authors: Chabrier, P E, Roubert, P, Lonchampt, M O, Plas, P, Braquet, P
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 15-09-1988; American Society for Biochemistry and Molecular Biology
• Subjects: angiotensin II; Angiotensin II - pharmacology; Animals; Atrial Natriuretic Factor; atrial natriuretic hormone; Biological and medical sciences; blood vessels; Cell receptors; Cell structures and functions; Fundamental and applied biological sciences. Psychology; Kinetics; Male; Molecular and cellular biology; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; Rats; Rats, Inbred Strains; Receptors, Atrial Natriuretic Factor; Receptors, Cell Surface - metabolism; smooth muscle; Time Factors; Cell culture; Vertebrata; Membrane receptor; Mammalia; Rat; Blood vessel; Rodentia; Molecular interaction; Smooth muscle; Atrial natriuretic peptide; Binding site; Angiotensin II
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)37691-9

Atrial natriuretic factor (ANF) is actively involved in the control of blood pressure and fluid homeostasis as a physiological antagonist of the renin-angiotensin system. To evaluate a possible interaction between ANF and angiotensin II (Ang-II) receptors, we investigated the effect of long term pretreatment (18 h) of rat cultured vascular smooth muscle cells with Ang-II. Binding of 125I-labeled ANF and cyclic GMP production induced by ANF were measured. After preincubation of the cells with Ang-II (1, 10, and 100 nM), the number of ANF binding sites (Bmax) was decreased by 30, 59, and 71%, respectively, with a slight decrease of the Kd values. Sar1-Ile8-Ang-II (100 nM), a specific Ang-II receptor antagonist, totally inhibited the down-regulation induced by Ang-II (10 nM). Moreover, the regulatory effect of Ang-II on ANF receptors appeared more slowly as compared to ANF homologous receptor regulation. Ang-II pretreatment did not desensitize but increased cyclic GMP production elicited by ANF, implying that only the number of non-guanylate cyclase-coupled receptors was affected. These findings, which were not observed with 100 nM of epinephrine, norepinephrine, histamine, serotonin, and Arg-vasopressin, demonstrate a specific and functional link between ANF and Ang-II receptors. This study also shows that the regulation of ANF receptors is heterogeneous, providing new evidence of multiple classes of ANF receptors.