Feasibility Study of a Human Papillomavirus E6 Oncoprotein Test for Diagnosis of Cervical Precancer and Cancer

• Published in: Journal of Clinical Microbiology Vol. 48; no. 12; pp. 4646 - 4648
• Main Authors: Schweizer, Johannes, Lu, Peter S, Mahoney, Charles W, Berard-Bergery, Marthe, Ho, Minh, Ramasamy, Valli, Silver, Jon E, Bisht, Arnima, Labiad, Yassine, Peck, Roger B, Lim, Jeanette, Jeronimo, Jose, Howard, Roslyn, Gravitt, Patti E, Castle, Philip E
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-12-2010; American Society for Microbiology (ASM)
• Subjects: Biological and medical sciences; Feasibility Studies; Female; Fundamental and applied biological sciences. Psychology; Human papillomavirus 16; Humans; Immunoassay - methods; Medical sciences; Microbiology; Miscellaneous; Oncogene Proteins, Viral - analysis; Papillomavirus Infections - complications; Papillomavirus Infections - diagnosis; Tumors; Uterine Cervical Neoplasms - diagnosis; Uterine Cervical Neoplasms - virology; Virology; Virology - methods; Premalignant lesion; Papovaviridae; Malignant tumor; Female genital diseases; Papillomavirus; Virus; Human papillomavirus; Oncoprotein; Diagnosis; Uterine cervix diseases; Cervical cancer; Cancer; Cervical precancerous lesion
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.01315-10

In a feasibility study using a prototype, lateral-flow test for human papillomavirus type 16, 18, and/or 45 (HPV16/18/45) E6 oncoproteins, 51 of 75 (68%; 95% confidence interval [95% CI] of 56 to 78%) of HPV16/18/45 DNA-positive specimens from women with a diagnosis of CIN3+ (cervical intraepithelial neoplasia grade 3+ or cervical cancer) tested positive for HPV16/18/45 E6 oncoprotein. None of 16 (95% CI of 0 to 37%) HPV16/18/45 DNA-positive cervical specimens from women with a negative or CIN1 diagnosis tested positive for HPV16/18/45 E6 oncoprotein.