Evidence that nebulin is a protein-ruler in muscle thin filaments

• Published in: FEBS letters Vol. 282; no. 2; pp. 313 - 316
• Main Authors: Labeit, S., Gibson, T., Lakey, A., Leonard, K., Zeviani, M., Knight, P., Wardale, J., Trinick, J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 06-05-1991; Elsevier
• Subjects: Actin Cytoskeleton - ultrastructure; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Cattle; Chickens; Contractile proteins; DNA - genetics; Fundamental and applied biological sciences. Psychology; Holoproteins; Humans; Molecular Sequence Data; Muscle Proteins - chemistry; Muscle Proteins - physiology; Nebulin; Protein-ruler; Proteins; Rabbits; Striated muscle; Thin filament; Protein-ruler; Thin filament; Nebulin; Striated muscle; Regulation(control); Vertebrata; Filament; Contractile protein; Joining; Comparative study; Aminoacid sequence
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(91)80503-U

Partial amino acid sequence was obtained from the massive myofibrillar protein nebulin. This consists of repeating motifs of about 35 residues and super-repeats of 7 × 35 = 245 residues. The repeat-motifs are likely to be largely α-helical and to interact with both actin and tropomyosin in thin filaments. Nebulin from different species was found to vary in size in proportion to filament length. The data are consistent with the proposal that nebulin acts as a protein-ruler to regulate precise thin filament assembly.