Betel quid-associated oral lesions and oral Candida species in a female Cambodian cohort

• Published in: Journal of oral pathology & medicine Vol. 31; no. 8; pp. 468 - 472
• Main Authors: Reichart, P. A., Schmidtberg, W., Samaranayake, L. P., Scheifele, C.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Munksgaard International Publishers 01-09-2002; Blackwell
• Subjects: Adult; Aged; Aged, 80 and over; Areca - adverse effects; betel quid chewing; Biological and medical sciences; Cambodia; Candida; Candida - classification; Candida - isolation & purification; Candida albicans - isolation & purification; Candida glabrata - isolation & purification; Candida tropicalis - isolation & purification; Chi-Square Distribution; Cohort Studies; Colony Count, Microbial; Dentistry; Ent and stomatologic mycoses; Female; Human mycoses; Humans; Infectious diseases; Leukoedema, Oral - etiology; Leukoplakia, Oral - etiology; Lichen Planus, Oral - etiology; Medical sciences; Middle Aged; Mouth Diseases - etiology; Mouth Diseases - microbiology; Mouth Mucosa - microbiology; Mouth Mucosa - pathology; Mycoses; Non tumoral diseases; oral carriage; Otorhinolaryngology. Stomatology; Palate - microbiology; Reagent Strips; Smoking - adverse effects; Statistics, Nonparametric; Time Factors; Tongue - microbiology; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology; Cambodia; Asia; Infection; Oral cavity; Candidiasis; Prevalence; Mycosis; Cohort study; Betel; Female; Oral cavity disease; Lesion; Epidemiology
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1034/j.1600-0714.2002.00009.x

Background:  Betel quid chewing (BQC) is still prevalent among elderly Cambodian women and is associated with a wide variety of oral mucosal lesions. BQC has also been associated with a reduced rate of dental caries and changes in the oral microbiological flora.
Methods:  Since no studies were available on the impact of BQC on the oral carriage of Candida species, in this study oral swabs (Fungiquick®, Hain Diagnostika, Germany) were taken from the tongue and palate of 48 Cambodian women with BQC habit (study group) and 13 control subjects without BQC habit (control group) to determine the spectrum of Candida species in these two groups. In addition, we investigated lesions of the oral mucosa likely to be associated with BQC habit in both study and control groups.
Results:  The median duration of BQC was 10 years (range 10 months−30 years). The following oral lesions were found in the study group: betel chewer's mucosa (85.4%), oral leukoplakia (8.3%), leukoedema (37.5%) and oral lichen planus (4.2%). Oral candidiasis was seen neither in BQ‐chewers nor in controls. Candida spp. were found in 70.8% of the cases (controls 69.2%). Whilst C. albicans was isolated from 27.1% of the study cohort, C. tropicalis was the second most common isolate. One control case was colonised by C. dubliniensis – the first report of this organism from a Cambodian population. There was no significant difference in the candidal carriage rate or the Candida species isolated between the study and the control group.
Conclusions:  Mycological findings from the present study do not indicate that BQC has a significant effect on oral colonisation by Candida species.