Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups

A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successf...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of Clinical Microbiology Vol. 49; no. 1; pp. 419 - 422
Main Authors:	Wu, Y, Tan, E.L, Yeo, A, Chan, K.P, Nishimura, H, Cardosa, M.J, Poh, C.L, Quak, S.H, Chow, Vincent T
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-01-2011 American Society for Microbiology (ASM)
Subjects:	Biological and medical sciences Enterovirus Enterovirus A, Human - genetics Enterovirus A, Human - isolation & purification Enterovirus Infections - diagnosis Enterovirus Infections - virology Fundamental and applied biological sciences. Psychology Humans Microbiology Microspheres Miscellaneous Molecular Sequence Data Oligonucleotides - genetics RNA, Viral - genetics Sequence Analysis, DNA Virology Virology - methods Virus Enterovirus 71 Enterovirus Identification Picornaviridae
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successfully detected and correctly subgenogrouped.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23 ObjectType-Feature-1 Current address: University of Malaya, Kuala Lumpur, Malaysia.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.01871-10