Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil
The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly...
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| Published in: | BMC veterinary research Vol. 12; no. 32; p. 31 |
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| Abstract | The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated.
Of the 802 samples analyzed, one milk (0.25%) and eight blood (2%) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30%) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB.
M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil. |
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| AbstractList | The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated.
Of the 802 samples analyzed, one milk (0.25%) and eight blood (2%) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30%) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB.
M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil. Background The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated. Results Of the 802 samples analyzed, one milk (0.25 %) and eight blood (2 %) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30 %) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB. Conclusions M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil. BACKGROUNDThe present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated.RESULTSOf the 802 samples analyzed, one milk (0.25%) and eight blood (2%) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30%) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB.CONCLUSIONSM. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil. |
| ArticleNumber | 31 |
| Audience | Academic |
| Author | Filho, Antônio Fernando Barbosa Batista Cezar, Renata Duarte da Silva de Oliveira, Pollyane Raysa Fernandes Arruda-Lima, Maíra Lúcio, Érica Chaves Pinheiro Junior, José Wilton Borges, Jonas de Melo Lucena-Silva, Norma Santana, Vania Lucia de Assis |
| Author_xml | – sequence: 1 givenname: Renata Duarte da Silva surname: Cezar fullname: Cezar, Renata Duarte da Silva email: natinhacezar@hotmail.com organization: Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE), Rua Dom Manuel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP 52171-900, Brazil. natinhacezar@hotmail.com – sequence: 2 givenname: Norma surname: Lucena-Silva fullname: Lucena-Silva, Norma email: norma.lucena@hotmail.com organization: Department of Immunology (Departamento de Imunologia), Research Center Aggeu Magalhães (Centro de Pesquisas Aggeu Magalhães-CPqAM) Oswaldo Cruz Foundation (Fundação Oswaldo Cruz - Fiocruz), Av. Professor Moraes Rego, s/n, Cidade Universitária, Recife, Pernambuco, CEP 50.740-465, Brazil. norma.lucena@hotmail.com – sequence: 3 givenname: Antônio Fernando Barbosa Batista surname: Filho fullname: Filho, Antônio Fernando Barbosa Batista email: afbbf@hotmail.com organization: Academic Unit of Garanhuns (Unidade Acadêmica de Garanhuns), Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE). Avenida Bom Pastor, s/n, Boa Vista, Garanhuns, Pernambuco, CEP 55292-270, Brazil. afbbf@hotmail.com – sequence: 4 givenname: Jonas de Melo surname: Borges fullname: Borges, Jonas de Melo email: jonas_borges1@hotmail.com organization: Academic Unit of Garanhuns (Unidade Acadêmica de Garanhuns), Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE). Avenida Bom Pastor, s/n, Boa Vista, Garanhuns, Pernambuco, CEP 55292-270, Brazil. jonas_borges1@hotmail.com – sequence: 5 givenname: Pollyane Raysa Fernandes surname: de Oliveira fullname: de Oliveira, Pollyane Raysa Fernandes email: pollyanne_raysa_fernandes@hotmail.com organization: Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE), Rua Dom Manuel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP 52171-900, Brazil. pollyanne_raysa_fernandes@hotmail.com – sequence: 6 givenname: Érica Chaves surname: Lúcio fullname: Lúcio, Érica Chaves email: erica.c.l@hotmail.com organization: Academic Unit of Garanhuns (Unidade Acadêmica de Garanhuns), Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE). Avenida Bom Pastor, s/n, Boa Vista, Garanhuns, Pernambuco, CEP 55292-270, Brazil. erica.c.l@hotmail.com – sequence: 7 givenname: Maíra surname: Arruda-Lima fullname: Arruda-Lima, Maíra email: maira_lima@hotmail.com organization: Department of Immunology (Departamento de Imunologia), Research Center Aggeu Magalhães (Centro de Pesquisas Aggeu Magalhães-CPqAM) Oswaldo Cruz Foundation (Fundação Oswaldo Cruz - Fiocruz), Av. Professor Moraes Rego, s/n, Cidade Universitária, Recife, Pernambuco, CEP 50.740-465, Brazil. maira_lima@hotmail.com – sequence: 8 givenname: Vania Lucia de Assis surname: Santana fullname: Santana, Vania Lucia de Assis email: vaniophillus@yahoo.com organization: National Agricultural Laboratory of Pernambuco (Laboratório Nacional Agropecuário de Pernambuco- Lanagro/PE), Ministry of Agriculture, Livestock and Food Supply of Brazil (Ministério da Agricultura, Pecuária e Abastecimento - MAPA). Rua Manoel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP 52171-030, Brazil. vaniophillus@yahoo.com – sequence: 9 givenname: José Wilton surname: Pinheiro Junior fullname: Pinheiro Junior, José Wilton email: wiltonjuniorufrpe@gmail.com organization: Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE), Rua Dom Manuel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP 52171-900, Brazil. wiltonjuniorufrpe@gmail.com |
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| Snippet | The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties... Background The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20... BACKGROUNDThe present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20... |
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| StartPage | 31 |
| SubjectTerms | Animals Brazil Cattle DNA, Bacterial - isolation & purification Female Genetic aspects Genotype Host-bacteria relationships Identification and classification Medical research Medicine, Experimental Milk - microbiology Molecular Diagnostic Techniques - veterinary Mycobacterium bovis Mycobacterium bovis - genetics Mycobacterium bovis - isolation & purification Physiological aspects Real-Time Polymerase Chain Reaction - veterinary Tuberculosis, Bovine - blood Tuberculosis, Bovine - microbiology |
| Title | Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/26897524 https://www.proquest.com/docview/1773730186 https://search.proquest.com/docview/1767626102 https://pubmed.ncbi.nlm.nih.gov/PMC4761206 |
| Volume | 12 |
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