Genome-Wide Analysis of PL7 Alginate Lyases in the Genus Zobellia

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 8; p. 2387
• Main Authors: Chernysheva, Nadezhda, Bystritskaya, Evgeniya, Likhatskaya, Galina, Nedashkovskaya, Olga, Isaeva, Marina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20-04-2021; MDPI
• Subjects: Algae; alginate utilization system; Alginates; Alginates - chemistry; Alginic acid; Bacteria; Enzymes; Evolutionary genetics; Flavobacteriaceae - classification; Flavobacteriaceae - enzymology; Flavobacteriaceae - genetics; Gene duplication; Gene transfer; Genes; Genetic variability; Genome, Bacterial - genetics; Genomes; Genomics; Horizontal transfer; orthologs; paralogs; Phylogenetics; polysaccharide lyase family 7; Polysaccharide-Lyases - genetics; Polysaccharides; Substrate Specificity; Zobellia; polysaccharide lyase family 7; alginate utilization system; genomes; Zobellia; orthologs; paralogs
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26082387

We carried out a detailed investigation of PL7 alginate lyases across the
genus. The main findings were obtained using the methods of comparative genomics and spatial structure modeling, as well as a phylogenomic approach. Initially, in order to elucidate the alginolytic potential of
, we calculated the content of polysaccharide lyase (PL) genes in each genome. The genus-specific PLs were PL1, PL6, PL7 (the most abundant), PL14, PL17, and PL40. We revealed that PL7 belongs to subfamilies 3, 5, and 6. They may be involved in local and horizontal gene transfer and gene duplication processes. Most likely, an individual evolution of PL7 genes promotes the genetic variability of the Alginate Utilization System across
. Apparently, the PL7 alginate lyases may acquire a sub-functionalization due to diversification between in-paralogs.