Molecular and biological diversity of HIV-1 in Brazil

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 87; no. 2; pp. 249 - 255
• Main Authors: Couto-Fernandez, J C, Morgado, M G, dos Santos, J I, Galvão-Castro, B
• Format: Journal Article
• Language: English
• Published: Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01-06-1992; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Acquired Immunodeficiency Syndrome - microbiology; AIDS/HIV; Brazil; Brazilian HIV; DNA, Viral - analysis; Genetic Variation; HIV-1 - genetics; HIV-1 - pathogenicity; human immunodeficiency virus 1; Humans; Male; PARASITOLOGY; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Population; restriction analysis; Restriction Mapping; TROPICAL MEDICINE; viral diversity; Virulence; Brazil; South America; Americas; Laboratory Examinations And Diagnoses; Viral Diseases; Biology; Hiv Infections; Examinations And Diagnoses; Diseases; Latin America; Genetic Technics; Hiv; Brazil; Genetics; Research Report; Developing Countries; Brazilian HIV; viral diversity; virulence; restriction analysis
• ISSN: 0074-0276; 1678-8060; 0074-0276; 1678-8060
• DOI: 10.1590/S0074-02761992000200013

To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, we analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment length polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.