Anticancer Effects of Propionic Acid Inducing Cell Death in Cervical Cancer Cells

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 16; p. 4951
• Main Authors: Pham, Chau Ha, Lee, Joo-Eun, Yu, Jinha, Lee, Sung Hoon, Yu, Kyung-Rok, Hong, Jaewoo, Cho, Namki, Kim, Seil, Kang, Dukjin, Lee, Soojin, Yoo, Hee Min
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16-08-2021; MDPI
• Subjects: Acetic acid; AKT protein; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Apoptosis; Autophagy; Autophagy - drug effects; Bacteria; Butyric acid; Cell Cycle - drug effects; Cell death; Cell Death - drug effects; Cell viability; Cervical cancer; Cervix; Colorectal cancer; Fatty acids; Female; Fermentation; Flow cytometry; Gastrointestinal system; Gastrointestinal tract; HeLa; HeLa Cells; Homeostasis; Human papillomavirus; Humans; Metabolites; Microbiota; Mitochondria; Mitochondrial Membranes - drug effects; Mitochondrial Membranes - metabolism; NF-kappa B - metabolism; NF-κB protein; Phagocytosis; Probiotics; Propionates - chemistry; Propionates - pharmacology; Propionic acid; Proteins; reactive oxygen species; Reactive Oxygen Species - metabolism; short-chain fatty acids; Signal Transduction - drug effects; TOR protein; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - pathology; Vagina; HeLa; short-chain fatty acids; cervical cancer; reactive oxygen species; propionic acid
• DOI: 10.3390/molecules26164951
• EISSN: 1420-3049

Recent studies found that short-chain fatty acids (SCFAs), which are produced through bacterial fermentation in the gastrointestinal tract, have oncoprotective effects against cervical cancer. The most common SCFAs that are well known include acetic acid, butyric acid, and propionic acid, among which propionic acid (PA) has been reported to induce apoptosis in HeLa cells. However, the mechanism in which SCFAs suppress HeLa cell viability remain poorly understood. Our study aims to provide a more detailed look into the mechanism of PA in HeLa cells. Flow cytometry analysis revealed that PA induces reactive oxygen species (ROS), leading to the dysfunction of the mitochondrial membrane. Moreover, PA inhibits NF-κB and AKT/mTOR signaling pathways and induces LC3B protein levels, resulting in autophagy. PA also increased the sub-G1 cell population that is characteristic of cell death. Therefore, the results of this study propose that PA inhibits HeLa cell viability through a mechanism mediated by the induction of autophagy. The study also suggests a new approach for cervical cancer therapeutics.