The Influence of Essential Oil Compounds on Antibacterial Activity of Mupirocin-Susceptible and Induced Low-Level Mupirocin-Resistant MRSA Strains

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 17; p. 3105
• Main Authors: Kwiatkowski, Paweł, Pruss, Agata, Wojciuk, Bartosz, Dołęgowska, Barbara, Wajs-Bonikowska, Anna, Sienkiewicz, Monika, Mężyńska, Monika, Łopusiewicz, Łukasz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27-08-2019; MDPI
• Subjects: Acetic acid; Anethole; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibacterial materials; Antibiotics; Antimicrobial activity; Antimicrobial agents; Bacteria; Carvacrol; Cineole; Drug resistance; Drug Synergism; essential oil compounds; Essential oils; Eucalyptol - isolation & purification; Eucalyptol - pharmacology; Eugenol; FTIR; Humans; Linalool; Linalyl acetate; low-level mupirocin-resistant; Menthol - isolation & purification; Menthol - pharmacology; Menthone; Methicillin-Resistant Staphylococcus aureus - drug effects; Microbial Viability - drug effects; Microorganisms; MRSA; Mucosa; Mupirocin; Mupirocin - pharmacology; mupirocin-susceptible; Mutation; Oils & fats; Oils, Volatile - isolation & purification; Oils, Volatile - pharmacology; Ointments; Pseudomonas fluorescens; Raman spectroscopy; Sepsis; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; Staphylococcus infections; Strains (organisms); synergistic activity; essential oil compounds; synergistic activity; FTIR; MRSA; low-level mupirocin-resistant; Raman spectroscopy; mupirocin-susceptible
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24173105

Because of the bacterial drug resistance development, it is reasonable to investigate chemical compounds capable of preventing the spread of resistance to mupirocin (MUP), commonly used in staphylococcal eradication. The objective of the study was to verify the influence of essential oil compounds (EOCs) on the antibacterial activity of MUP against mupirocin-susceptible (MupS) and induced low-level mupirocin-resistant (MupRL) methicillin-resistant
(MRSA) strains. The following parameters were examined: MRSA
and MRSA
susceptibility to EOCs (1,8-cineole, eugenol, carvacrol, linalool, (-)-menthone, linalyl acetate, and
-anethole), the bacterial cell size distribution, and chemical composition by the use of Fourier Transform Infrared Spectroscopy (FTIR) and Raman spectroscopies. The MRSA
and MRSA
strains were susceptible to all tested EOCs. 1,8-cineole and (-)-menthone showed synergistic activity against MRSA
in combination with mupirocin, whereas 1,8-cineole exhibited synergistic activity against MRSA
as well. In-depth analysis showed that both MRSA
and MRSA
displayed similar distributions of the bacterial cell size. The FTIR and Raman spectra of the MRSA
and MRSA
strains showed differences in some regions. New bands in the MRSA
Raman spectrum were observed. It was concluded that the use of 1,8-cineole in combination with mupirocin can increase the mupirocin activity against the MRSA
and MRSA
strains.