Exceptional Properties of Lepidium sativum L. Extract and Its Impact on Cell Viability, Ros Production, Steroidogenesis, and Intracellular Communication in Mice Leydig Cells In Vitro

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 16; p. 5127
• Main Authors: Jambor, Tomas, Zajickova, Terezia, Arvay, Julius, Ivanisova, Eva, Tirdilova, Ivana, Knizatova, Nikola, Greifova, Hana, Kovacik, Anton, Galova, Eliska, Lukac, Norbert
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11-08-2022; MDPI
• Subjects: Animals; Antioxidants; Antioxidants - metabolism; Antioxidants - pharmacology; Benzoic acid; Biological activity; Cell Communication; Cell membranes; Cell Survival; Cell viability; Chromatography; Communication; Ethanol; Fertility; Ferulic acid; Flavonoids; In vitro fertilization; Infertility; Intracellular; intracellular communication; Intracellular signalling; Lepidium; Lepidium - metabolism; Lepidium sativum; Lepidium sativum - chemistry; Lepidium sativum L; Leydig cells; Leydig Cells - metabolism; Male; Mice; Morphology; Motility; Plant Extracts - metabolism; Plant Extracts - pharmacology; Polyphenols; reactive oxygen species; Resveratrol; Sperm; Steroidogenesis; Steroids; Testosterone - metabolism; viability; Slovakia; St Louis Missouri; United Kingdom > UK; United States > US; Germany; steroidogenesis; Lepidium sativum L; intracellular communication; Leydig cells; reactive oxygen species; viability
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27165127

The prevalence of reproductive dysfunction in males has risen in the last few years, and alternative therapies are gradually gaining in popularity. Our in vitro study aimed to evaluate the potential impact of
L. on mice TM3 Leydig cells, concerning basal parameters such as cell viability, cell membrane integrity, and lysosomal activity, after 24 h and 48 h exposure. Moreover, reactive oxygens species generation, sex-steroid hormone secretion, and intercellular communication were quantified. In the present study, the microgreen extract from Lepidium was rich in ferulic acid, 4-OH benzoic acid, and resveratrol, with a significant antioxidant activity. The results showed that lower experimental doses (62.5-250 µg/mL) could positively affect the observed parameters, with significant differences at 250 µg/mL after 24 h and 48 h, respectively. Potential risks could be associated with higher concentrations, starting at 500 µg/mL, 1000 µg/mL, and 2000 µg/mL of
Nevertheless, biochemical quantification indicated a significant antioxidant potential and a rich content of biologically active molecules at the applied doses, and time determined the intracellular response of the cultured model.