Antioxidant capacity and phenolic content of Caesalpinia pyramidalis Tul. and Sapium glandulosum (L.) Morong from Northeastern Brazil

• Published in: Molecules (Basel, Switzerland) Vol. 16; no. 6; pp. 4728 - 4739
• Main Authors: da Silva, Carlos Henrique Tabosa Pereira, Sobrinho, Tadeu José da Silva Peixoto, e Castro, Valérium Thijan Nobre de Almeida, Lima, Danielle da Cunha Amaral, de Amorim, Elba Lúcia Cavalcanti
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07-06-2011; MDPI
• Subjects: Antioxidants; Antioxidants - analysis; Brazil; Caesalpinia - chemistry; Caesalpinia pyramidalis; DPPH assay; Ethanol; FIC assay; Flavonoids; Flavonoids - analysis; Folk medicine; Metabolites; Phenolic compounds; Phenols - analysis; Plant Extracts - analysis; Plant Leaves - chemistry; Plant Stems - chemistry; Sapium - chemistry; Sapium glandulosum; Tannins - analysis; Brazil
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules16064728

The aims of this study were to quantify the phenolic content and evaluate the antioxidant potential of extracts from the bark and leaves of C. pyramidalis and S. glandulosum. The total phenolic content (TPC) and total tannin content (TTC) were determined using the Folin-Ciocalteu method, and the total flavonoids content (TFC) was measured via complexation with aluminum chloride. The antioxidant activity was evaluated with DPPH (2.2-diphenyl-1-picrylhydrazyl) and FIC (ferrous ion chelating) assays. The TPC ranged between 135.55 ± 9.85 and 459.79 ± 11.65 tannic acid equivalents (TAE) in mg/g material (mg TAE/g). The leaves of both species contained high levels of tannins and flavonoids. The crude ethanol extracts (CEE) from the bark of C. pyramidalis showed high antioxidant activity when compared to ascorbic acid and rutin, whereas the CEE from the leaves was more efficient in chelating ferrous ions. C. pyramidalis had very high phenolic content and anti-radical activity, which indicates a need for further studies aimed at the purification and identification of compounds responsible for the antioxidant activity.