How the concentration of insulin affects the development of preantral follicles in goats

• Published in: Cell and tissue research Vol. 346; no. 3; pp. 451 - 456
• Main Authors: Chaves, R. N, Alves, A. M. C. V, Faustino, L. R, Oliveira, K. P. L, Campello, C. C, Lopes, C. A. P, Báo, S. N, Figueiredo, J. R
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01-12-2011; Springer; Springer Nature B.V
• Subjects: animal ovaries; Animals; Biomedical and Life Sciences; Biomedicine; Cellular biology; culture media; Diabetes therapy; Female; Goats; Human Genetics; Insulin; Insulin - pharmacology; Molecular Medicine; oocytes; Ovarian cancer; Ovarian Follicle - drug effects; Ovarian Follicle - growth & development; Proteomics; Reproductive system; Short Communication; ultrastructure; viability; Zoology; Ovary; Goat; Insulin; Viability; Preantral follicle
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/s00441-011-1264-9

This study investigated the effect of adding different insulin concentrations to the culture medium for goat preantral follicle development in vitro. The ovarian fragments were immediately fixed or cultured for 7 days in MEM with insulin (0, 5, 10 ng/ml and 5 or 10 μg/ml). The results showed that, after 7 days of culture, insulin at 10 ng/ml was the best concentration to preserve follicular viability and ultrastructure, resulting in the highest rates of normal follicles. After 7 days, only treatments with 10 ng/ml and 5 μg/ml of insulin increased follicular activation when compared to other concentrations. Regarding follicular and oocyte growth, the presence of 10 ng/ml of insulin promoted a larger diameter than other treatments. In conclusion, this study shows that addition of 10 ng/ml of insulin to the culture medium improved the survival and stimulated growth of goat preantral follicles.