Biodiversity of carbapenem-resistant bacteria in clinical samples from the Southwest Amazon region (Rondônia/Brazil)

• Published in: Scientific reports Vol. 14; no. 1; p. 9383
• Main Authors: dos Santos, Levy Assis, Cayô, Rodrigo, Valiatti, Tiago Barcelos, Gales, Ana Cristina, de Araújo, Larissa Fatarelli Bento, Rodrigues, Fernando Marques, de Carvalho, Tatiane Silva, Vaz, Marcos André Braz, Campanharo, Marcela
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23-04-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/158/670; 631/326/107; 631/326/1762; 631/326/22/1434; 631/326/325/1506; Acinetobacter baumannii - drug effects; Acinetobacter baumannii - genetics; Acinetobacter baumannii - isolation & purification; Anti-Bacterial Agents - pharmacology; Antimicrobial resistance; Bacteria; Bacteria - classification; Bacteria - drug effects; Bacteria - genetics; Bacterial Proteins - genetics; beta-Lactamases - genetics; Biodiversity; Brazil; Carbapenemases; Carbapenems; Carbapenems - pharmacology; CRE bacteria; Decision making; Drug Resistance, Bacterial - genetics; Epidemiology; Genetic variability; Humanities and Social Sciences; Humans; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - isolation & purification; Laboratories; Microbial Sensitivity Tests; Multi-drug resistant bacteria; multidisciplinary; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - isolation & purification; Public health; Public policy; Retrospective Studies; Science; Science (multidisciplinary); Surveillance epidemiological; Brazil; Amazon Basin; Multi-drug resistant bacteria; Surveillance epidemiological; Antimicrobial resistance; Public health; Carbapenemases
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-59733-w

Brazil is recognized for its biodiversity and the genetic variability of its organisms. This genetic variability becomes even more valuable when it is properly documented and accessible. Understanding bacterial diversity through molecular characterization is necessary as it can improve patient treatment, reduce the length of hospital stays and the selection of resistant bacteria, and generate data for health and epidemiological surveillance. In this sense, in this study, we aimed to understand the biodiversity and molecular epidemiology of carbapenem-resistant bacteria in clinical samples recovered in the state of Rondônia, located in the Southwest Amazon region. Retrospective data from the Central Public Health Laboratories (LACEN/RO) between 2018 and 2021 were analysed using the Laboratory Environment Manager Platform (GAL). Seventy-two species with carbapenem resistance profiles were identified, of which 25 species carried at least one gene encoding carbapenemases of classes A (
bla
KPC
-like), B (
bla
NDM
-like,
bla
SPM
-like or
bla
VIM
-like) and D (
bla
OXA-23
-like,
bla
OXA-24
-like,
bla
OXA-48
-like,
bla
OXA-58
-like or
bla
OXA-143
-like), among which we will highlight
Klebsiella pneumoniae
,
Pseudomonas aeruginosa
,
Acinetobacter baumannii
,
Serratia marcescens
, and
Providencia
spp. With these results, we hope to contribute to the field by providing epidemiological molecular data for state surveillance on bacterial resistance and assisting in public policy decision-making.