Genetic Characterization of Streptococcus pyogenes emm89 Strains Isolated in Japan From 2011 to 2019

Abstract Invasive infection caused by Streptococcus pyogenes emm 89 strains has been increasing in several countries linked to a recently emergent clade of emm 89 strains, designated clade 3. In Japan, the features of emm 89 S. pyogenes strains, such as clade classification, remains unknown. In this...

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Published in:	Infectious microbes & diseases Vol. 2; no. 4; pp. 160 - 166
Main Authors:	Hirose, Yujiro, Yamaguchi, Masaya, Takemoto, Norihiko, Miyoshi-Akiyama, Tohru, Sumitomo, Tomoko, Nakata, Masanobu, Ikebe, Tadayoshi, Hanada, Tomoki, Yamaguchi, Takahiro, Kawahara, Ryuji, Okuno, Rumi, Otsuka, Hitoshi, Matsumoto, Yuko, Terashima, Yuji, Kazawa, Yu, Nakanishi, Noriko, Uchida, Kaoru, Akiyama, Yumi, Iwabuchi, Kaori, Nakagawa, Chikara, Yamamoto, Kazunari, Nizet, Victor, Kawabata, Shigetada
Format:	Journal Article
Language:	English
Published:	Hagerstown, MD Lippincott Williams & Wilkins 01-12-2020 Wolters Kluwer Health
Subjects:	Original
Online Access:	Get full text
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Summary:	Abstract Invasive infection caused by Streptococcus pyogenes emm 89 strains has been increasing in several countries linked to a recently emergent clade of emm 89 strains, designated clade 3. In Japan, the features of emm 89 S. pyogenes strains, such as clade classification, remains unknown. In this study, we collected emm 89 strains isolated from both streptococcal toxic shock syndrome (STSS) (89 STSS isolates) and noninvasive infections (72 non-STSS isolates) in Japan from 2011 to 2019, and conducted whole-genome sequencing and comparative analysis, which resulted in classification of a large majority into clade 3 regardless of disease severity. In addition, invasive disease-associated factors were found among emm 89 strains, including mutations of control of virulence sensor, and absence of the hylP1 gene encoding hyaluronidase. These findings provide new insights into genetic features of emm 89 strains.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 How to cite this article: Hirose Y, Yamaguchi M, Takemoto N, Miyoshi-Akiyama T, Sumitomo T, Nakata M, Ikebe T, Hanada T, Yamaguchi T, Kawahara R, Okuno R, Otsuka H, Matsumoto Y, Terashima Y, Kazawa Y, Nakanishi N, Uchida K, Akiyama Y, Iwabuchi K, Nakagawa C, Yamamoto K, Nizet V, Kawabata S. Genetic characterization of Streptococcus pyogenes emm89 strains isolated in Japan From 2011 to 2019. Infect Microb Dis. 2020;2(4):160–166. https://doi.org/10.1097/IM9.0000000000000038 Conflicts of Interest: The authors reported no conflict of interest. Funding: This work was supported in part by AMED (Grant numbers: JP19fk0108044, JP19fm0208007), Japanese Society for the Promotion of Science (JSPS) KAKENHI (Grant numbers: 19H03825, 20K18474), JSPS Overseas Research Fellowships, Takeda Science Foundation, Japanese Association for Oral Biology Grant in Aid for Young Scientists, SECOM Science and Technology Foundation, The Naito Foundation, and Kobayashi International Scholarship Foundation. The funders had no role in study design, data collection or analysis, decision to publish, or preparation of the manuscript. Author contributions: YH, TI and SK designed the study; YH, NT, TM and TH performed DNA sequencing and bioinformatics analysis; YH, TS and MN performed enzyme assay; YH, TY, RK, RO, HO, YM, YT, YK, NN, KU, YA, KI, CN and KY collected clinical strains; YH, MY, TS, MN, YM, NN, TM, VN and SK wrote the manuscript; all authors reviewed and approved the final manuscript.
ISSN:	2641-5917 2641-5917
DOI:	10.1097/IM9.0000000000000038