Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects

• Published in: BioMed research international Vol. 2015; no. 2015; pp. 1 - 10
• Main Authors: Ortíz-Hernández, Laura, Gaytán-Colín, Paul, Dantán-González, Edgar, Sánchez-Rodríguez, Federico, Guillén-Solís, Gabriel, Martínez-Garduño, Felipe, Hernández-Mendoza, Armando, Martínez-Ocampo, Fernando, Obregón-Barboza, Verónica, Mendoza-Mejía, Ared, Quiroz-Castañeda, Rosa Estela, Peña-Chora, Guadalupe
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2015; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Alcaligenes faecalis; Alcaligenes faecalis - genetics; Alcaligenes faecalis - isolation & purification; Alcaligenes faecalis - pathogenicity; Animals; Bacteria; Bacteriology; Biological Products - pharmacology; Deoxyribonucleic acid; DNA; DNA sequencing; Enzymes; Galleria mellonella; Genetic aspects; Genomes; Health aspects; Host-bacteria relationships; Larva - drug effects; Larva - microbiology; Maximum likelihood method; Methods; Moths - drug effects; Moths - microbiology; Motility; Nematoda; Nucleotide sequencing; Observations; Pest Control, Biological - methods; Phylogenetics; Phylogeny; Proteins; Proteobacteria; Servers; Steinernema feltiae; Toxicity
• DOI: 10.1155/2015/570243
• EISSN: 2314-6141
• ISSN: 2314-6133

We report the isolation of a bacterium from Galleria mellonella larva and its identification using genome sequencing and phylogenomic analysis. This bacterium was named Alcaligenes faecalis strain MOR02. Microscopic analyses revealed that the bacteria are located in the esophagus and intestine of the nematodes Steinernema feltiae, S. carpocapsae, and H. bacteriophora. Using G. mellonella larvae as a model, when the larvae were injected with 24,000 CFU in their hemocoel, more than 96% mortality was achieved after 24 h. Additionally, toxicity assays determined that 1 μg of supernatant extract from A. faecalis MOR02 killed more than 70% G. mellonella larvae 96 h after injection. A correlation of experimental data with sequence genome analyses was also performed. We discovered genes that encode proteins and enzymes that are related to pathogenicity, toxicity, and host/environment interactions that may be responsible for the observed phenotypic characteristics. Our data demonstrates that the bacteria are able to use different strategies to colonize nematodes and kill insects to their own benefit. However, there remains an extensive group of unidentified microorganisms that could be participating in the infection process. Additionally, a nematode-bacterium association could be established probably as a strategy of dispersion and colonization.