Development and Validation of High-performance Thin Layer Chromatographic Method for Ursolic Acid in Malus domestica Peel

• Published in: Indian journal of pharmaceutical sciences Vol. 75; no. 4; pp. 486 - 489
• Main Authors: Nikam, P H, Kareparamban, J A, Jadhav, A P, Kadam, V J
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01-07-2013; Medknow Publications & Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd
• Subjects: Analytical chemistry; Apple; Chemical properties; Chromatography; Fruits; Identification and classification; Methods; Physiological aspects; Phytochemistry; Short Communication; Studies; Terpenes; Thin layer chromatography; India; Mumbai India; Malus domestica; quantification; validation; HPTLC; ursolic acid
• ISSN: 0250-474X; 1998-3743
• DOI: 10.4103/0250-474X.119815

Ursolic acid, a pentacyclic triterpenoid possess a wide range of pharmacological activities. It shows hypoglycemic, antiandrogenic, antibacterial, antiinflammatory, antioxidant, diuretic and cynogenic activity. It is commonly present in plants especially coating of leaves and fruits, such as apple fruit, vinca leaves, rosemary leaves, and eucalyptus leaves. A simple high-performance thin layer chromatographic method has been developed for the quantification of ursolic acid from apple peel (Malus domestica). The samples dissolved in methanol and linear ascending development was carried out in twin trough glass chamber. The mobile phase was selected as toluene:ethyl acetate:glacial acetic acid (70:30:2). The linear regression analysis data for the calibration plots showed good linear relationship with r(2)=0.9982 in the concentration range 0.2-7 μg/spot with respect to peak area. According to the ICH guidelines the method was validated for linearity, accuracy, precision, and robustness. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of ursolic acid.