Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity

• Published in: Journal of lipid research Vol. 47; no. 10; pp. 2171 - 2178
• Main Authors: Yoneyama, Kelly A.G., Tanaka, Ameria K., Silveira, Thais G.V., Takahashi, Helio K., Straus, Anita H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-10-2006; Elsevier
• Subjects: Animals; Centrifugation, Density Gradient; detergent-resistant membranes; Detergents - chemistry; Detergents - pharmacology; gas chromatography-mass spectrometry; glycolipid; Glycosylphosphatidylinositols - analysis; Glycosylphosphatidylinositols - immunology; Immunity, Innate; inositol phosphorylceramide; Leishmania braziliensis - cytology; Leishmania braziliensis - drug effects; Leishmania braziliensis - physiology; lipid raft; Macrophages - parasitology; Membrane Microdomains - chemistry; Membrane Microdomains - drug effects; methyl-β-cyclodextrin; Mice; Mice, Inbred BALB C; Microscopy, Electron, Transmission; promastigote; Solubility; glycolipid; lipid raft; gas chromatography-mass spectrometry; methyl-β-cyclodextrin; inositol phosphorylceramide; promastigote; detergent-resistant membranes
• ISSN: 0022-2275; 1539-7262
• DOI: 10.1194/jlr.M600285-JLR200

Detergent-resistant membranes (DRMs) from Leishmania (Viannia) braziliensis promastigotes, insoluble in 1% Triton X-100 at 4°C, were fractionated by sucrose density gradient ultracentrifugation. They were composed of glycoinositolphospholipids (GIPLs), inositol phosphorylceramide (IPC), phosphatidylinositol (PI), phosphatidylethanolamine (PE), and sterols. In contrast, 1% Triton X-100-soluble fraction was composed of PE, phosphatidylcholine, phosphatidylserine, PI, IPC, sterol, and lyso-PI. High-performance thin-layer chromatography (HPTLC) immunostaining using monoclonal antibody SST-1 showed that 85% of GIPLs are present in DRMs, and immunoelectron microscopic analysis showed that SST-1-reactive components are located in patches along the parasite surface. No difference in GIPL pattern was observed by HPTLC between Triton X-100-soluble versus -insoluble fractions at 4°C. Analysis of fatty acid composition in DRMs by GC-MS showed the presence of GIPLs containing an alkylacylglycerol, presenting mainly saturated acyl and alkyl chains. DRMs also contained sterol, IPC with saturated fatty acids, PI with at least one saturated acyl chain, and PE with predominantly oleic acid. Promastigotes treated with methyl-β-cyclodextrin to disrupt lipid microdomains showed significantly lower macrophage infectivity, suggesting a relationship between lipid microdomains and the infectivity of these parasites.