Prevalence and genotyping of human isolates of Giardia duodenalis from Albania
Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125 stool samples collected from children living in the urban and the rural areas of Tirana (Albania) and hospitalized with acute gastroenteritis. 7...
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Published in: | Parasitology international Vol. 55; no. 4; pp. 295 - 297 |
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Abstract | Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of
Giardia duodenalis from 125 stool samples collected from children living in the urban and the rural areas of Tirana (Albania) and hospitalized with acute gastroenteritis. 7 out of 125 samples resulted positive for
Giardia at the microscopic examination (5.6%). In 50 selected samples including the 7 samples positive for
Giardia by microscopy, 3 and 15 additional positive samples were detected by immunofluorescence and PCR, respectively. Seasonality appeared as an important parameter to be evaluated in order to better understand the prevalence of infection. Sequence analysis revealed both human Assemblage A and B. This result represents the first data on
G. duodenalis genotypes in Albania. |
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AbstractList | Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of
Giardia duodenalis from 125 stool samples collected from children living in the urban and the rural areas of Tirana (Albania) and hospitalized with acute gastroenteritis. 7 out of 125 samples resulted positive for
Giardia at the microscopic examination (5.6%). In 50 selected samples including the 7 samples positive for
Giardia by microscopy, 3 and 15 additional positive samples were detected by immunofluorescence and PCR, respectively. Seasonality appeared as an important parameter to be evaluated in order to better understand the prevalence of infection. Sequence analysis revealed both human Assemblage A and B. This result represents the first data on
G. duodenalis genotypes in Albania. Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125 stool samples collected from children living in the urban and the rural areas of Tirana (Albania) and hospitalized with acute gastroenteritis. 7 out of 125 samples resulted positive for Giardia at the microscopic examination (5.6%). In 50 selected samples including the 7 samples positive for Giardia by microscopy, 3 and 15 additional positive samples were detected by immunofluorescence and PCR, respectively. Seasonality appeared as an important parameter to be evaluated in order to better understand the prevalence of infection. Sequence analysis revealed both human Assemblage A and B. This result represents the first data on G. duodenalis genotypes in Albania. |
Author | Di Cave, D. D'Orazi, C. Berrilli, F. Xhelilaj, L. Çaça, P. Cenko, F. Bebeci, D. Divizia, M. Donia, D. Orecchia, P. Bejko, D. |
Author_xml | – sequence: 1 givenname: F. surname: Berrilli fullname: Berrilli, F. email: berrilli@uniroma2.it organization: Department of Public Health and Cell Biology, Faculty of Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, Italy – sequence: 2 givenname: D. surname: Di Cave fullname: Di Cave, D. organization: Department of Public Health and Cell Biology, Faculty of Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, Italy – sequence: 3 givenname: C. surname: D'Orazi fullname: D'Orazi, C. organization: Azienda Ospedaliera Universitaria Policlinico “Tor Vergata”, Rome, Italy – sequence: 4 givenname: P. surname: Orecchia fullname: Orecchia, P. organization: Department of Public Health and Cell Biology, Faculty of Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, Italy – sequence: 5 givenname: L. surname: Xhelilaj fullname: Xhelilaj, L. organization: University Hospital Centre “Mother Theresa”, Paediatric Unit, Tirana, Albania – sequence: 6 givenname: D. surname: Bejko fullname: Bejko, D. organization: University Hospital Centre “Mother Theresa”, Paediatric Unit, Tirana, Albania – sequence: 7 givenname: P. surname: Çaça fullname: Çaça, P. organization: University Hospital Centre “Mother Theresa”, Paediatric Unit, Tirana, Albania – sequence: 8 givenname: D. surname: Bebeci fullname: Bebeci, D. organization: University Hospital Centre “Mother Theresa”, Paediatric Unit, Tirana, Albania – sequence: 9 givenname: F. surname: Cenko fullname: Cenko, F. organization: Albanian Child Survival Project, American Red Cross, Tirana, Albania – sequence: 10 givenname: D. surname: Donia fullname: Donia, D. organization: Department of Public Health and Cell Biology, Faculty of Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, Italy – sequence: 11 givenname: M. surname: Divizia fullname: Divizia, M. organization: Department of Public Health and Cell Biology, Faculty of Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, Italy |
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Cites_doi | 10.1016/S0304-4017(02)00322-9 10.2307/3284227 10.1007/s10096-004-1149-x 10.1002/jmv.20229 10.1016/S0020-7519(00)00127-2 10.1016/S0020-7519(01)00340-X 10.1016/j.meegid.2003.08.003 10.1016/S0020-7519(01)00183-7 10.1016/j.vetpar.2004.09.008 10.2166/wst.2001.0716 10.1086/498169 10.1093/nar/25.24.4876 10.2166/wst.2004.0018 10.1016/0169-4758(93)90223-3 10.2307/3284315 |
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Giardia duodenalis from 125... Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125... |
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SubjectTerms | Albania Albania - epidemiology Animals Child Feces - parasitology Female Gastroenteritis - epidemiology Gastroenteritis - parasitology Genotype Genotyping Geography Giardia - classification Giardia - genetics Giardia - isolation & purification Giardia duodenalis Giardiasis - diagnosis Giardiasis - epidemiology Giardiasis - parasitology Humans Male Prevalence Seasons Sex Factors |
Title | Prevalence and genotyping of human isolates of Giardia duodenalis from Albania |
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