Prevalence and genotyping of human isolates of Giardia duodenalis from Albania

Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125 stool samples collected from children living in the urban and the rural areas of Tirana (Albania) and hospitalized with acute gastroenteritis. 7...

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Published in:Parasitology international Vol. 55; no. 4; pp. 295 - 297
Main Authors: Berrilli, F., Di Cave, D., D'Orazi, C., Orecchia, P., Xhelilaj, L., Bejko, D., Çaça, P., Bebeci, D., Cenko, F., Donia, D., Divizia, M.
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Language:English
Published: Netherlands Elsevier Ireland Ltd 01-12-2006
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Abstract Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125 stool samples collected from children living in the urban and the rural areas of Tirana (Albania) and hospitalized with acute gastroenteritis. 7 out of 125 samples resulted positive for Giardia at the microscopic examination (5.6%). In 50 selected samples including the 7 samples positive for Giardia by microscopy, 3 and 15 additional positive samples were detected by immunofluorescence and PCR, respectively. Seasonality appeared as an important parameter to be evaluated in order to better understand the prevalence of infection. Sequence analysis revealed both human Assemblage A and B. This result represents the first data on G. duodenalis genotypes in Albania.
AbstractList Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125 stool samples collected from children living in the urban and the rural areas of Tirana (Albania) and hospitalized with acute gastroenteritis. 7 out of 125 samples resulted positive for Giardia at the microscopic examination (5.6%). In 50 selected samples including the 7 samples positive for Giardia by microscopy, 3 and 15 additional positive samples were detected by immunofluorescence and PCR, respectively. Seasonality appeared as an important parameter to be evaluated in order to better understand the prevalence of infection. Sequence analysis revealed both human Assemblage A and B. This result represents the first data on G. duodenalis genotypes in Albania.
Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125 stool samples collected from children living in the urban and the rural areas of Tirana (Albania) and hospitalized with acute gastroenteritis. 7 out of 125 samples resulted positive for Giardia at the microscopic examination (5.6%). In 50 selected samples including the 7 samples positive for Giardia by microscopy, 3 and 15 additional positive samples were detected by immunofluorescence and PCR, respectively. Seasonality appeared as an important parameter to be evaluated in order to better understand the prevalence of infection. Sequence analysis revealed both human Assemblage A and B. This result represents the first data on G. duodenalis genotypes in Albania.
Author Di Cave, D.
D'Orazi, C.
Berrilli, F.
Xhelilaj, L.
Çaça, P.
Cenko, F.
Bebeci, D.
Divizia, M.
Donia, D.
Orecchia, P.
Bejko, D.
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  organization: University Hospital Centre “Mother Theresa”, Paediatric Unit, Tirana, Albania
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Keywords Giardia duodenalis
Genotyping
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Snippet Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125...
Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125...
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SubjectTerms Albania
Albania - epidemiology
Animals
Child
Feces - parasitology
Female
Gastroenteritis - epidemiology
Gastroenteritis - parasitology
Genotype
Genotyping
Geography
Giardia - classification
Giardia - genetics
Giardia - isolation & purification
Giardia duodenalis
Giardiasis - diagnosis
Giardiasis - epidemiology
Giardiasis - parasitology
Humans
Male
Prevalence
Seasons
Sex Factors
Title Prevalence and genotyping of human isolates of Giardia duodenalis from Albania
URI https://dx.doi.org/10.1016/j.parint.2006.06.002
https://www.ncbi.nlm.nih.gov/pubmed/16875862
https://search.proquest.com/docview/19373469
https://search.proquest.com/docview/69019359
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