Novel Methodology for Creating Macaque Retinas with Sortable Photoreceptors and Ganglion Cells

The ability to generate macaque retinas with sortable cell populations would be of great benefit to both basic and translational studies of the primate retina. The purpose of our study was therefore to develop methods to achieve this goal by selectively labeling, in life, photoreceptors (PRs) and re...

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Published in:	Frontiers in neuroscience Vol. 10; p. 551
Main Authors:	Choudhury, Shreyasi, Strang, Christianne E, Alexander, John J, Scalabrino, Miranda L, Lynch Hill, Julie, Kasuga, Daniel T, Witherspoon, C Douglas, Boye, Sanford L, Gamlin, Paul D, Boye, Shannon E
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Research Foundation 01-12-2016 Frontiers Media S.A
Subjects:	adeno associated virus (AAV) Disease Expression vectors Flow cytometry Fluorescent indicators Gene expression Gene therapy Kinases lateral geniculate nuclei (LGN) injection Lateral geniculate nucleus macaque Methods Neuroscience Photoreceptors photoreceptors (PRs) Phylogeny Primates Retina Retinal ganglion cells retinal ganglion cells (RGCs) Retrograde transport subretinal injection Tropism Vectors (Biology) United States > US macaque fluorescent activated cell sorting (FACS) subretinal injection photoreceptors (PRs) retinal ganglion cells (RGCs) lateral geniculate nuclei (LGN) injection adeno associated virus (AAV)
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Abstract	The ability to generate macaque retinas with sortable cell populations would be of great benefit to both basic and translational studies of the primate retina. The purpose of our study was therefore to develop methods to achieve this goal by selectively labeling, in life, photoreceptors (PRs) and retinal ganglion cells (RGCs) with separate fluorescent markers. Labeling of macaque ( ) PRs and RGCs was accomplished by subretinal delivery of AAV5-hGRK1-GFP, and retrograde transport of micro-ruby™ from the lateral geniculate nucleus, respectively. Retinas were anatomically separated into different regions. Dissociation conditions were optimized, and cells from each region underwent fluorescent activated cell sorting (FACS). Expression of retinal cell type- specific genes was assessed by quantitative real-time PCR to characterize isolated cell populations. We show that macaque PRs and RGCs can be simultaneously labeled in-life and enriched populations isolated by FACS. Recovery from different retinal regions indicated efficient isolation/enrichment for PRs and RGCs, with the macula being particularly amendable to this technique. The methods and materials presented here allow for the identification of novel reagents designed to target RGCs and/or photoreceptors in a species that is phylogenetically and anatomically similar to human. These techniques will enable screening of intravitreally-delivered AAV capsid libraries for variants with increased tropism for PRs and/or RGCs and the evaluation of vector tropism and/or cellular promoter activity of gene therapy vectors in a clinically relevant species.
AbstractList	The ability to generate macaque retinas with sortable cell populations would be of great benefit to both basic and translational studies of the primate retina. The purpose of our study was therefore to develop methods to achieve this goal by selectively labeling, in life, photoreceptors (PRs) and retinal ganglion cells (RGCs) with separate fluorescent markers. Labeling of macaque ( ) PRs and RGCs was accomplished by subretinal delivery of AAV5-hGRK1-GFP, and retrograde transport of micro-ruby™ from the lateral geniculate nucleus, respectively. Retinas were anatomically separated into different regions. Dissociation conditions were optimized, and cells from each region underwent fluorescent activated cell sorting (FACS). Expression of retinal cell type- specific genes was assessed by quantitative real-time PCR to characterize isolated cell populations. We show that macaque PRs and RGCs can be simultaneously labeled in-life and enriched populations isolated by FACS. Recovery from different retinal regions indicated efficient isolation/enrichment for PRs and RGCs, with the macula being particularly amendable to this technique. The methods and materials presented here allow for the identification of novel reagents designed to target RGCs and/or photoreceptors in a species that is phylogenetically and anatomically similar to human. These techniques will enable screening of intravitreally-delivered AAV capsid libraries for variants with increased tropism for PRs and/or RGCs and the evaluation of vector tropism and/or cellular promoter activity of gene therapy vectors in a clinically relevant species. Purpose: The ability to generate macaque retinas with sortable cell populations would be of great benefit to both basic and translational studies of the primate retina. The purpose of our study was therefore to develop methods to achieve this goal by selectively labeling, in life, photoreceptors (PRs) and retinal ganglion cells (RGCs) with separate fluorescent markers. Methods: Labeling of macaque ( Macaca fascicularis ) PRs and RGCs was accomplished by subretinal delivery of AAV5-hGRK1-GFP, and retrograde transport of micro-ruby™ from the lateral geniculate nucleus, respectively. Retinas were anatomically separated into different regions. Dissociation conditions were optimized, and cells from each region underwent fluorescent activated cell sorting (FACS). Expression of retinal cell type- specific genes was assessed by quantitative real-time PCR to characterize isolated cell populations. Results: We show that macaque PRs and RGCs can be simultaneously labeled in-life and enriched populations isolated by FACS. Recovery from different retinal regions indicated efficient isolation/enrichment for PRs and RGCs, with the macula being particularly amendable to this technique. Conclusions: The methods and materials presented here allow for the identification of novel reagents designed to target RGCs and/or photoreceptors in a species that is phylogenetically and anatomically similar to human. These techniques will enable screening of intravitreally-delivered AAV capsid libraries for variants with increased tropism for PRs and/or RGCs and the evaluation of vector tropism and/or cellular promoter activity of gene therapy vectors in a clinically relevant species. Purpose: The ability to generate macaque retinas with sortable cell populations would be of great benefit to both basic and translational studies of the primate retina. The purpose of our study was therefore to develop methods to achieve this goal by selectively labeling, in life, photoreceptors (PRs) and retinal ganglion cells (RGCs) with separate fluorescent markers. Methods: Labeling of macaque (Macaca fascicularis) PRs and RGCs was accomplished by subretinal delivery of AAV5-hGRK1-GFP, and retrograde transport of micro-ruby™ from the lateral geniculate nucleus, respectively. Retinas were anatomically separated into different regions. Dissociation conditions were optimized, and cells from each region underwent fluorescent activated cell sorting (FACS). Expression of retinal cell type- specific genes was assessed by quantitative real-time PCR to characterize isolated cell populations. Results: We show that macaque PRs and RGCs can be simultaneously labeled in-life and enriched populations isolated by FACS. Recovery from different retinal regions indicated efficient isolation/enrichment for PRs and RGCs, with the macula being particularly amendable to this technique. Conclusions: The methods and materials presented here allow for the identification of novel reagents designed to target retinal ganglion cells and/or photoreceptors in a species that is phylogenetically and anatomically similar to human. These techniques will enable screening of intravitreally- delivered AAV capsid libraries for variants with increased tropism for PRs and/or RGCs and the evaluation of vector tropism and/or cellular promoter activity of gene therapy vectors in a clinically relevant species.
Author	Kasuga, Daniel T Strang, Christianne E Witherspoon, C Douglas Gamlin, Paul D Boye, Sanford L Scalabrino, Miranda L Boye, Shannon E Alexander, John J Lynch Hill, Julie Choudhury, Shreyasi
AuthorAffiliation	4 Department of Ophthalmology, University of Alabama at Birmingham Birmingham, AL, USA 2 Department of Psychology, University of Alabama at Birmingham Birmingham, AL, USA 1 Department of Ophthalmology, University of Florida Gainesville, FL, USA 3 Department of Human Genetics, Emory University Atlanta, GA, USA
AuthorAffiliation_xml	– name: 3 Department of Human Genetics, Emory University Atlanta, GA, USA – name: 4 Department of Ophthalmology, University of Alabama at Birmingham Birmingham, AL, USA – name: 2 Department of Psychology, University of Alabama at Birmingham Birmingham, AL, USA – name: 1 Department of Ophthalmology, University of Florida Gainesville, FL, USA
Author_xml	– sequence: 1 givenname: Shreyasi surname: Choudhury fullname: Choudhury, Shreyasi organization: Department of Ophthalmology, University of Florida Gainesville, FL, USA – sequence: 2 givenname: Christianne E surname: Strang fullname: Strang, Christianne E organization: Department of Psychology, University of Alabama at Birmingham Birmingham, AL, USA – sequence: 3 givenname: John J surname: Alexander fullname: Alexander, John J organization: Department of Human Genetics, Emory University Atlanta, GA, USA – sequence: 4 givenname: Miranda L surname: Scalabrino fullname: Scalabrino, Miranda L organization: Department of Ophthalmology, University of Florida Gainesville, FL, USA – sequence: 5 givenname: Julie surname: Lynch Hill fullname: Lynch Hill, Julie organization: Department of Ophthalmology, University of Alabama at Birmingham Birmingham, AL, USA – sequence: 6 givenname: Daniel T surname: Kasuga fullname: Kasuga, Daniel T organization: Department of Ophthalmology, University of Alabama at Birmingham Birmingham, AL, USA – sequence: 7 givenname: C Douglas surname: Witherspoon fullname: Witherspoon, C Douglas organization: Department of Ophthalmology, University of Alabama at Birmingham Birmingham, AL, USA – sequence: 8 givenname: Sanford L surname: Boye fullname: Boye, Sanford L organization: Department of Ophthalmology, University of Florida Gainesville, FL, USA – sequence: 9 givenname: Paul D surname: Gamlin fullname: Gamlin, Paul D organization: Department of Human Genetics, Emory University Atlanta, GA, USA – sequence: 10 givenname: Shannon E surname: Boye fullname: Boye, Shannon E organization: Department of Ophthalmology, University of Florida Gainesville, FL, USA
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Keywords	macaque fluorescent activated cell sorting (FACS) subretinal injection photoreceptors (PRs) retinal ganglion cells (RGCs) lateral geniculate nuclei (LGN) injection adeno associated virus (AAV)
Language	English
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Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Stylianos Michalakis, Ludwig Maximilian University of Munich, Germany This article was submitted to Neurodegeneration, a section of the journal Frontiers in Neuroscience Joint senior authors. Reviewed by: Claudio Punzo, University of Massachusetts Medical School, USA; Marius Ader, Dresden University of Technology, Germany
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Snippet	The ability to generate macaque retinas with sortable cell populations would be of great benefit to both basic and translational studies of the primate retina.... Purpose: The ability to generate macaque retinas with sortable cell populations would be of great benefit to both basic and translational studies of the... Purpose: The ability to generate macaque retinas with sortable cell populations would be of great benefit to both basic and translational studies of the...
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SubjectTerms	adeno associated virus (AAV) Disease Expression vectors Flow cytometry Fluorescent indicators Gene expression Gene therapy Kinases lateral geniculate nuclei (LGN) injection Lateral geniculate nucleus macaque Methods Neuroscience Photoreceptors photoreceptors (PRs) Phylogeny Primates Retina Retinal ganglion cells retinal ganglion cells (RGCs) Retrograde transport subretinal injection Tropism Vectors (Biology)
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Title	Novel Methodology for Creating Macaque Retinas with Sortable Photoreceptors and Ganglion Cells
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