Hierarchical Cluster Analysis of Three-Dimensional Reconstructions of Unbiased Sampled Microglia Shows not Continuous Morphological Changes from Stage 1 to 2 after Multiple Dengue Infections in Callithrix penicillata

It is known that microglial morphology and function are related, but few studies have explored the subtleties of microglial morphological changes in response to specific pathogens. In the present report we quantitated microglia morphological changes in a monkey model of dengue disease with virus CNS...

Full description

Saved in:

Bibliographic Details
Published in:	Frontiers in neuroanatomy Vol. 10; p. 23
Main Authors:	Diniz, Daniel G, Silva, Geane O, Naves, Thaís B, Fernandes, Taiany N, Araújo, Sanderson C, Diniz, José A P, de Farias, Luis H S, Sosthenes, Marcia C K, Diniz, Cristovam G, Anthony, Daniel C, da Costa Vasconcelos, Pedro F, Picanço Diniz, Cristovam W
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Research Foundation 22-03-2016 Frontiers Media S.A
Subjects:	Animals Antigens Biodiversity Brain research Callithrix penicillata Cluster analysis Dengue fever dengue virus infection Dentate Gyrus Encephalitis Genotypes hierarchical cluster analysis Immunoglobulins Infections Microglia microglial morphological subtleties Morphology Neuropathology Neuroscience Phenotypes Principal components analysis Serotypes Tumor necrosis factor-α Vectors United States > US dentate gyrus hierarchical cluster analysis dengue virus infection Callithrix penicillata microglial morphological subtleties
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	It is known that microglial morphology and function are related, but few studies have explored the subtleties of microglial morphological changes in response to specific pathogens. In the present report we quantitated microglia morphological changes in a monkey model of dengue disease with virus CNS invasion. To mimic multiple infections that usually occur in endemic areas, where higher dengue infection incidence and abundant mosquito vectors carrying different serotypes coexist, subjects received once a week subcutaneous injections of DENV3 (genotype III)-infected culture supernatant followed 24 h later by an injection of anti-DENV2 antibody. Control animals received either weekly anti-DENV2 antibodies, or no injections. Brain sections were immunolabeled for DENV3 antigens and IBA-1. Random and systematic microglial samples were taken from the polymorphic layer of dentate gyrus for 3-D reconstructions, where we found intense immunostaining for TNFα and DENV3 virus antigens. We submitted all bi- or multimodal morphological parameters of microglia to hierarchical cluster analysis and found two major morphological phenotypes designated types I and II. Compared to type I (stage 1), type II microglia were more complex; displaying higher number of nodes, processes and trees and larger surface area and volumes (stage 2). Type II microglia were found only in infected monkeys, whereas type I microglia was found in both control and infected subjects. Hierarchical cluster analysis of morphological parameters of 3-D reconstructions of random and systematic selected samples in control and ADE dengue infected monkeys suggests that microglia morphological changes from stage 1 to stage 2 may not be continuous.
AbstractList	It is known that microglial morphology and function are related, but few studies have explored the subtleties of microglial morphological changes in response to specific pathogens. In the present report we quantitated microglia morphological changes in a monkey model of dengue disease with virus CNS invasion. To mimic multiple infections that usually occur in endemic areas, where higher dengue infection incidence and abundant mosquito vectors carrying different serotypes coexist, subjects received once a week subcutaneous injections of DENV3 (genotype III)-infected culture supernatant followed 24 h later by an injection of anti-DENV2 antibody. Control animals received either weekly anti-DENV2 antibodies, or no injections. Brain sections were immunolabeled for DENV3 antigens and IBA-1. Random and systematic microglial samples were taken from the polymorphic layer of dentate gyrus for 3-D reconstructions, where we found intense immunostaining for TNFα and DENV3 virus antigens. We submitted all bi- or multimodal morphological parameters of microglia to hierarchical cluster analysis and found two major morphological phenotypes designated types I and II. Compared to type I (stage 1), type II microglia were more complex; displaying higher number of nodes, processes and trees and larger surface area and volumes (stage 2). Type II microglia were found only in infected monkeys, whereas type I microglia was found in both control and infected subjects. Hierarchical cluster analysis of morphological parameters of 3-D reconstructions of random and systematic selected samples in control and ADE dengue infected monkeys suggests that microglia morphological changes from stage 1 to stage 2 may not be continuous. It is known that microglial morphology and function are related, but few studies have explored the subtleties of microglial morphological changes in response to specific pathogens. In the present report we quantitated microglia morphological changes in a monkey model of dengue disease with virus CNS invasion. To mimic multiple infections that usually occur in endemic areas, where higher dengue infection incidence and abundant mosquito vectors carrying different serotypes coexist, subjects received once a week subcutaneous injections of DENV3 (genotype III)-infected culture supernatant followed 24 hours later by an injection of anti-DENV2 antibody. Control animals received either weekly anti-DENV2 antibodies, or no injections. Brain sections were immunolabeled for DENV3 antigens and IBA-1. Random and systematic microglial samples were taken from the polymorphic layer of dentate gyrus for 3-D reconstructions, where we found intense immunostaining for TNFα and DENV3 virus antigens. We submitted all bi- or multimodal morphological parameters of microglia to hierarchical cluster analysis and found two major morphological phenotypes designated types I and II. Compared to type I (stage 1), type II microglia were more complex; displaying higher number of nodes, processes and trees and larger surface area and volumes (stage 2). Type II microglia were found only in infected monkeys, whereas type I microglia was found in both control and infected subjects. Hierarchical cluster analysis of morphological parameters of 3-D reconstructions of random and systematic selected samples in control and ADE dengue infected monkeys suggests that microglia morphological changes from stage 1 to stage 2 may not be continuous.
Author	Fernandes, Taiany N Sosthenes, Marcia C K Diniz, Cristovam G Naves, Thaís B Araújo, Sanderson C Diniz, José A P da Costa Vasconcelos, Pedro F Anthony, Daniel C Diniz, Daniel G Silva, Geane O de Farias, Luis H S Picanço Diniz, Cristovam W
AuthorAffiliation	6 Laboratório de Biologia Molecular e Ambiental, Instituto Federal de Educação Ciência e Tecnologia do Pará, Campus de Bragança, Bragança Pará, Brasil 7 Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas Ananindeua, Brasil 2 Experimental Neuropathology Laboratory, Department of Pharmacology, University of Oxford Oxford, UK 5 Instituto de Ciências Biológicas, Universidade Federal do Pará Belém, Brasil 1 Laboratório de Investigações em Neurodegeneração e Infecção, Instituto de Ciências Biológicas, Universidade Federal do Pará, Hospital Universitário João de Barros Barreto Belém, Brasil 4 Departamento de Microscopia Eletrônica, Instituto Evandro Chagas Belém, Brasil 3 Curso de Graduação em Biologia, Universidade da Amazônia Belém, Brasil
AuthorAffiliation_xml	– name: 2 Experimental Neuropathology Laboratory, Department of Pharmacology, University of Oxford Oxford, UK – name: 1 Laboratório de Investigações em Neurodegeneração e Infecção, Instituto de Ciências Biológicas, Universidade Federal do Pará, Hospital Universitário João de Barros Barreto Belém, Brasil – name: 3 Curso de Graduação em Biologia, Universidade da Amazônia Belém, Brasil – name: 4 Departamento de Microscopia Eletrônica, Instituto Evandro Chagas Belém, Brasil – name: 6 Laboratório de Biologia Molecular e Ambiental, Instituto Federal de Educação Ciência e Tecnologia do Pará, Campus de Bragança, Bragança Pará, Brasil – name: 7 Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas Ananindeua, Brasil – name: 5 Instituto de Ciências Biológicas, Universidade Federal do Pará Belém, Brasil
Author_xml	– sequence: 1 givenname: Daniel G surname: Diniz fullname: Diniz, Daniel G organization: Laboratório de Investigações em Neurodegeneração e Infecção, Instituto de Ciências Biológicas, Universidade Federal do Pará, Hospital Universitário João de Barros BarretoBelém, Brasil; Experimental Neuropathology Laboratory, Department of Pharmacology, University of OxfordOxford, UK – sequence: 2 givenname: Geane O surname: Silva fullname: Silva, Geane O organization: Laboratório de Investigações em Neurodegeneração e Infecção, Instituto de Ciências Biológicas, Universidade Federal do Pará, Hospital Universitário João de Barros Barreto Belém, Brasil – sequence: 3 givenname: Thaís B surname: Naves fullname: Naves, Thaís B organization: Laboratório de Investigações em Neurodegeneração e Infecção, Instituto de Ciências Biológicas, Universidade Federal do Pará, Hospital Universitário João de Barros Barreto Belém, Brasil – sequence: 4 givenname: Taiany N surname: Fernandes fullname: Fernandes, Taiany N organization: Curso de Graduação em Biologia, Universidade da Amazônia Belém, Brasil – sequence: 5 givenname: Sanderson C surname: Araújo fullname: Araújo, Sanderson C organization: Departamento de Microscopia Eletrônica, Instituto Evandro Chagas Belém, Brasil – sequence: 6 givenname: José A P surname: Diniz fullname: Diniz, José A P organization: Departamento de Microscopia Eletrônica, Instituto Evandro Chagas Belém, Brasil – sequence: 7 givenname: Luis H S surname: de Farias fullname: de Farias, Luis H S organization: Instituto de Ciências Biológicas, Universidade Federal do Pará Belém, Brasil – sequence: 8 givenname: Marcia C K surname: Sosthenes fullname: Sosthenes, Marcia C K organization: Laboratório de Investigações em Neurodegeneração e Infecção, Instituto de Ciências Biológicas, Universidade Federal do Pará, Hospital Universitário João de Barros Barreto Belém, Brasil – sequence: 9 givenname: Cristovam G surname: Diniz fullname: Diniz, Cristovam G organization: Laboratório de Biologia Molecular e Ambiental, Instituto Federal de Educação Ciência e Tecnologia do Pará, Campus de Bragança, Bragança Pará, Brasil – sequence: 10 givenname: Daniel C surname: Anthony fullname: Anthony, Daniel C organization: Experimental Neuropathology Laboratory, Department of Pharmacology, University of Oxford Oxford, UK – sequence: 11 givenname: Pedro F surname: da Costa Vasconcelos fullname: da Costa Vasconcelos, Pedro F organization: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas Ananindeua, Brasil – sequence: 12 givenname: Cristovam W surname: Picanço Diniz fullname: Picanço Diniz, Cristovam W organization: Laboratório de Investigações em Neurodegeneração e Infecção, Instituto de Ciências Biológicas, Universidade Federal do Pará, Hospital Universitário João de Barros BarretoBelém, Brasil; Experimental Neuropathology Laboratory, Department of Pharmacology, University of OxfordOxford, UK
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/27047345$$D View this record in MEDLINE/PubMed
BookMark	eNpdkk1v1DAQhiNURD_gzglZ4sJli-3YcXJBqrZAV2qFRNuzNXHsxJXXXmwH6D_l5-DdLVXLaayZdx7bM-9xdeCD11X1luDTum67j8aDh1OKSXOKMab1i-qINA1dcEK7gyfnw-o4pTuMG9pw_qo6pAIzUTN-VP25sDpCVJNV4NDSzSnriM48uPtkEwoG3UxR68W5XWufbCgF9F2r4FOOs8olsRPd-t5C0gO6hvXGlXhlVQyjs4Cup_ArIR8yWgafrZ_DnNBViJspuDDur53AjzohE8MaXWcYNSIoB0QRmO1zrmaXbcGic-3HWaOVN_rhbuvREpyzeYr2N9pob5V1DjK8rl4acEm_eYgn1e2XzzfLi8Xlt6-r5dnlQrEO5wUlg6FAG9G0bSs6AUz0rSC655xoYJx30LYGcMcwo6pv-rpRAEoYUDWpDa1PqtWeOwS4k5to1xDvZQArd4kQRwkxW-W0NKrviRGGcCEYCNPyQdFBKYFF33esLqxPe9Zm7td6UNrnCO4Z9HnF20mO4adkLSZtQwrgwwMghh-zTlmubVK6DMTrMnZJhOgYYbwWRfr-P-ldmGNZb5KUdpw3hAhWVHivKttMKWrz-BiC5daCcmdBubWg3FmwtLx7-onHhn-eq_8CncPfuA
CitedBy_id	crossref_primary_10_3389_fncel_2017_00235 crossref_primary_10_3390_brainsci11081009 crossref_primary_10_3389_fncel_2017_00233 crossref_primary_10_1111_ejn_14920 crossref_primary_10_3389_fimmu_2021_683026
Cites_doi	10.1038/nn1997 10.1016/j.jneumeth.2005.01.009 10.1177/1073858414530512 10.1038/ncb1201-1035 10.1128/JVI.00220-10 10.1016/S0166-2236(98)01362-9 10.1002/cne.903430304 10.1016/j.bbi.2013.12.010 10.1007/s00216-010-3754-0 10.1016/S0301-0082(98)00069-0 10.1046/j.1460-9568.1998.00379.x 10.1111/nan.12011 10.1371/journal.pone.0097096 10.1186/1742-2094-11-12 10.1007/s10930-012-9411-z 10.3389/fncel.2013.00003 10.1016/j.chom.2010.08.007 10.1093/cercor/bhr229 10.1006/exnr.2000.7575 10.1002/cne.22652 10.1016/0304-3940(88)90346-1 10.1016/S0065-3527(03)60011-4 10.1523/JNEUROSCI.4363-08.2009 10.1126/science.1110647 10.1002/cne.23228 10.1007/s00429-014-0802-0 10.1177/1721727X1301100315 10.1016/j.jchemneu.2014.10.001 10.1111/neup.12229 10.1016/j.neuroscience.2012.07.029 10.1371/journal.pone.0038971 10.1016/j.jneumeth.2007.01.009 10.1016/0361-9230(94)90189-9 10.1038/srep00051 10.1002/cne.10858 10.1371/journal.ppat.1000790 10.1016/S1385-299X(96)00014-1 10.1371/journal.ppat.1004031 10.1016/j.bbi.2012.12.017 10.1111/ejn.12951 10.1002/1098-1136(200103)33:3256::AID-GLIA10243.0.CO;2-J 10.1016/j.neuro.2012.01.012 10.1212/WNL.0b013e3182a4a577 10.1590/S0074-02762012000800010 10.1093/cercor/bhs151 10.1523/JNEUROSCI.2061-14.2015 10.3389/fncel.2013.00070
ContentType	Journal Article
Copyright	2016. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Copyright © 2016 Diniz, Silva, Naves, Fernandes, Araújo, Diniz, de Farias, Sosthenes, Diniz, Anthony, da Costa Vasconcelos and Picanço Diniz. 2016 Diniz, Silva, Naves, Fernandes, Araújo, Diniz, de Farias, Sosthenes, Diniz, Anthony, da Costa Vasconcelos and Picanço Diniz
Copyright_xml	– notice: 2016. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: Copyright © 2016 Diniz, Silva, Naves, Fernandes, Araújo, Diniz, de Farias, Sosthenes, Diniz, Anthony, da Costa Vasconcelos and Picanço Diniz. 2016 Diniz, Silva, Naves, Fernandes, Araújo, Diniz, de Farias, Sosthenes, Diniz, Anthony, da Costa Vasconcelos and Picanço Diniz
DBID	NPM AAYXX CITATION 3V. 7XB 88I 8FE 8FH 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO GNUQQ HCIFZ LK8 M2P M7P PIMPY PQEST PQQKQ PQUKI PRINS Q9U 7X8 5PM DOA
DOI	10.3389/fnana.2016.00023
DatabaseName	PubMed CrossRef ProQuest Central (Corporate) ProQuest Central (purchase pre-March 2016) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central ProQuest Central Essentials Biological Science Collection ProQuest Central ProQuest Natural Science Collection ProQuest One Community College ProQuest Central Korea ProQuest Central Student SciTech Premium Collection (Proquest) (PQ_SDU_P3) Biological Sciences Science Database Biological Science Database Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals
DatabaseTitle	PubMed CrossRef Publicly Available Content Database ProQuest Science Journals (Alumni Edition) ProQuest Central Student ProQuest Biological Science Collection ProQuest Central Basic ProQuest Central Essentials ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection Biological Science Database ProQuest SciTech Collection ProQuest Central China ProQuest Central ProQuest One Academic UKI Edition Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest One Academic ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	PubMed Publicly Available Content Database
Database_xml	– sequence: 1 dbid: DOA name: Directory of Open Access Journals url: http://www.doaj.org/ sourceTypes: Open Website
DeliveryMethod	fulltext_linktorsrc
Discipline	Anatomy & Physiology
EISSN	1662-5129
EndPage	23
ExternalDocumentID	oai_doaj_org_article_fcbb1f7f15774a7f85dc2dcc707bb943 10_3389_fnana_2016_00023 27047345
Genre	Journal Article
GeographicLocations	United States--US
GeographicLocations_xml	– name: United States--US
GrantInformation_xml	– fundername: Financiadora de Estudos e Projetos grantid: 01.04.0043.00 – fundername: Conselho Nacional de Desenvolvimento Científico e Tecnológico grantid: 300460/2005-8; 301955/2007-7; 200065/2014-9; 471444/2006-5
GroupedDBID	--- 29H 2WC 53G 5GY 5VS 88I 8FE 8FH 9T4 AAFWJ ABUWG ACGFO ACGFS ACXDI ADBBV ADRAZ AEGXH AENEX AFKRA AFPKN AIAGR ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ C1A CCPQU CS3 DIK DWQXO E3Z EBS EJD EMOBN F5P GNUQQ GROUPED_DOAJ GX1 HCIFZ HYE IAO IEA IHR IPNFZ ISR KQ8 LK8 M2P M48 M7P M~E NPM O5R O5S OK1 P2P PGMZT PIMPY PQQKQ PROAC RIG RNS RPM TR2 AAYXX CITATION 3V. 7XB 8FK PQEST PQUKI PRINS Q9U 7X8 5PM
ID	FETCH-LOGICAL-c490t-21df2a2676888797a47b871eb551ea4559a88fa094042cb6b36caac7fac313f23
IEDL.DBID	RPM
ISSN	1662-5129
IngestDate	Tue Oct 22 14:38:41 EDT 2024 Tue Sep 17 21:23:53 EDT 2024 Sat Oct 26 01:21:02 EDT 2024 Thu Oct 10 18:55:51 EDT 2024 Thu Sep 26 17:12:43 EDT 2024 Sat Sep 28 08:04:43 EDT 2024
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Keywords	dentate gyrus hierarchical cluster analysis dengue virus infection Callithrix penicillata microglial morphological subtleties
Language	English
License	Copyright © 2016 Diniz, Silva, Naves, Fernandes, Araújo, Diniz, de Farias, Sosthenes, Diniz, Anthony, da Costa Vasconcelos and Picanço Diniz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c490t-21df2a2676888797a47b871eb551ea4559a88fa094042cb6b36caac7fac313f23
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Jihane Homman-Ludiye, Monash University, Australia; Karl Daniel Murray, University of California, Davis, USA Edited by: Pablo Blinder, Tel Aviv University, Israel
OpenAccessLink	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801861/
PMID	27047345
PQID	2295561174
PQPubID	4424405
PageCount	1
ParticipantIDs	doaj_primary_oai_doaj_org_article_fcbb1f7f15774a7f85dc2dcc707bb943 pubmedcentral_primary_oai_pubmedcentral_nih_gov_4801861 proquest_miscellaneous_1779414537 proquest_journals_2295561174 crossref_primary_10_3389_fnana_2016_00023 pubmed_primary_27047345
PublicationCentury	2000
PublicationDate	2016-03-22
PublicationDateYYYYMMDD	2016-03-22
PublicationDate_xml	– month: 03 year: 2016 text: 2016-03-22 day: 22
PublicationDecade	2010
PublicationPlace	Switzerland
PublicationPlace_xml	– name: Switzerland – name: Lausanne
PublicationTitle	Frontiers in neuroanatomy
PublicationTitleAlternate	Front Neuroanat
PublicationYear	2016
Publisher	Frontiers Research Foundation Frontiers Media S.A
Publisher_xml	– name: Frontiers Research Foundation – name: Frontiers Media S.A
References	22355570 - Sci Rep. 2011;1:51 11781564 - Nat Cell Biol. 2001 Dec;3(12):1035-42 11170719 - Exp Neurol. 2001 Mar;168(1):32-46 22824429 - Neuroscience. 2012 Dec 6;225:162-71 24830807 - PLoS One. 2014 May 15;9(5):e97096 9385054 - Brain Res Brain Res Protoc. 1997 Feb;1(1):100-8 20490471 - Anal Bioanal Chem. 2010 Jul;397(5):1853-9 23295753 - Mem Inst Oswaldo Cruz. 2012 Dec;107(8):1021-9 12949777 - J Comp Neurol. 2003 Oct 13;465(2):161-3 15831717 - Science. 2005 May 27;308(5726):1314-8 24447857 - J Neuroinflammation. 2014 Jan 21;11:12 14689700 - Adv Virus Res. 2003;60:421-67 23946308 - Neurology. 2013 Sep 17;81(12):1079-88 21491430 - J Comp Neurol. 2011 Oct 1;519(14):2803-10 11241743 - Glia. 2001 Mar 1;33(3):256-66 25462387 - J Chem Neuroanat. 2014 Nov;61-62:176-88 23305936 - Brain Behav Immun. 2013 Mar;29:136-46 22987820 - J Comp Neurol. 2013 Apr 1;521(5):1184-201 3374833 - Neurosci Lett. 1988 Feb 29;85(2):169-71 25788673 - J Neurosci. 2015 Mar 18;35(11):4552-70 21123382 - J Virol. 2011 Feb;85(4):1671-83 25980955 - Eur J Neurosci. 2015 Aug;42(4):2036-50 22701737 - PLoS One. 2012;7(6):e38971 10221782 - Prog Neurobiol. 1999 Apr;57(6):563-81 26303046 - Neuropathology. 2016 Feb;36(1):3-16 24412599 - Brain Behav Immun. 2014 Mar;37:1-14 23720611 - Front Cell Neurosci. 2013 May 15;7:70 24722525 - Neuroscientist. 2015 Apr;21(2):169-84 8027448 - J Comp Neurol. 1994 May 15;343(3):370-86 15935220 - J Neurosci Methods. 2005 Jul 15;146(1):50-60 9786222 - Eur J Neurosci. 1998 Oct;10(10):3284-90 22322212 - Neurotoxicology. 2012 Mar;33(2):191-206 23252647 - Neuropathol Appl Neurobiol. 2013 Feb;39(1):3-18 19339593 - J Neurosci. 2009 Apr 1;29(13):3974-80 22562126 - Protein J. 2012 Jun;31(5):366-73 23386810 - Front Cell Neurosci. 2013 Jan 30;7:3 10092043 - Trends Neurosci. 1999 Feb;22(2):51-61 24699622 - PLoS Pathog. 2014 Apr 03;10(4):e1004031 21878486 - Cereb Cortex. 2012 Jun;22(6):1442-54 8193937 - Brain Res Bull. 1994;34(1):73-8 24878824 - Brain Struct Funct. 2015 Jul;220(4):2423-39 20168989 - PLoS Pathog. 2010 Feb 12;6(2):e1000790 17965659 - Nat Neurosci. 2007 Nov;10(11):1387-94 20833378 - Cell Host Microbe. 2010 Sep 16;8(3):271-83 22710611 - Cereb Cortex. 2013 Aug;23(8):1784-97 17368561 - J Neurosci Methods. 2007 May 15;162(1-2):206-14 Hanisch (B18) 2007; 10 Saper (B34) 2003; 465 Soltys (B37) 2005; 146 Beynon (B5) 2012; 225 Nimmerjahn (B30) 2005; 308 Stence (B38) 2001; 33 Diniz (B13) 2013; 13 Aich (B1) 2012; 31 West (B46) 1999; 22 Balsitis (B2) 2010; 6 Diniz (B14) 2012; 107 Beltramello (B3) 2010; 8 Friberg (B15) 2011; 1 Kloss (B25) 2001; 168 de Sousa (B12) 2015; 42 Schweitzer (B35) 1997; 1 Taylor (B40) 2014; 9 Ullrich (B42) 2001; 3 Heppner (B20) 1998; 10 Kolb (B26) 1994; 343 Couch (B10) 2013; 29 Shu (B36) 1988; 85 Boche (B6) 2013; 39 Ng (B29) 2014; 10 Streit (B39) 1999; 57 Davis (B11) 1994; 34 Yamada (B47) 2013; 521 Wake (B44) 2009; 29 Miyamoto (B28) 2013; 7 Gomez-Nicola (B16) 2015; 21 Harry (B19) 2012; 33 Hinwood (B21) 2012; 22 Boonnak (B7) 2011; 85 Carlo (B8) 2011; 519 Li (B27) 2010; 397 Hinwood (B22) 2013; 23 Hosseini-Sharifabad (B23) 2007; 162 Karperien (B24) 2013; 7 Papageorgiou (B31) 2015; 220 Santos-Filho (B33) 2014; 61–62 Torres-Platas (B41) 2014; 11 Pillai (B32) 2012; 7 Benarroch (B4) 2013; 81 Clark (B9) 2015; 35 Halstead (B17) 2003; 60 Vasconcelos (B43) 2016; 36 Walker (B45) 2014; 37
References_xml	– volume: 10 start-page: 1387 year: 2007 ident: B18 article-title: Microglia: active sensor and versatile effector cells in the normal and pathologic brain publication-title: Nat. Neurosci. doi: 10.1038/nn1997 contributor: fullname: Hanisch – volume: 146 start-page: 50 year: 2005 ident: B37 article-title: Quantitative morphological study of microglial cells in the ischemic rat brain using principal component analysis publication-title: J. Neurosci. Methods doi: 10.1016/j.jneumeth.2005.01.009 contributor: fullname: Soltys – volume: 21 start-page: 169 year: 2015 ident: B16 article-title: Microglial dynamics and role in the healthy and diseased brain: a paradigm of functional plasticity publication-title: Neuroscientist doi: 10.1177/1073858414530512 contributor: fullname: Gomez-Nicola – volume: 3 start-page: 1035 year: 2001 ident: B42 article-title: Regulation of microglial expression of integrins by poly(ADP-ribose) polymerase-1 publication-title: Nat. Cell Biol. doi: 10.1038/ncb1201-1035 contributor: fullname: Ullrich – volume: 85 start-page: 1671 year: 2011 ident: B7 article-title: Cell type specificity and host genetic polymorphisms influence antibody-dependent enhancement of dengue virus infection publication-title: J. Virol. doi: 10.1128/JVI.00220-10 contributor: fullname: Boonnak – volume: 22 start-page: 51 year: 1999 ident: B46 article-title: Stereological methods for estimating the total number of neurons and synapses: issues of precision and bias publication-title: Trends Neurosci. doi: 10.1016/S0166-2236(98)01362-9 contributor: fullname: West – volume: 343 start-page: 370 year: 1994 ident: B26 article-title: Are there three types of horizontal cell in the human retina? publication-title: J. Comp. Neurol. doi: 10.1002/cne.903430304 contributor: fullname: Kolb – volume: 37 start-page: 1 year: 2014 ident: B45 article-title: Dynamic structural remodelling of microglia in health and disease: a review of the models, the signals and the mechanisms publication-title: Brain Behav. Immun. doi: 10.1016/j.bbi.2013.12.010 contributor: fullname: Walker – volume: 397 start-page: 1853 year: 2010 ident: B27 article-title: An improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells publication-title: Anal. Bioanal. Chem. doi: 10.1007/s00216-010-3754-0 contributor: fullname: Li – volume: 57 start-page: 563 year: 1999 ident: B39 article-title: Reactive microgliosis publication-title: Prog. Neurobiol. Aging doi: 10.1016/S0301-0082(98)00069-0 contributor: fullname: Streit – volume: 10 start-page: 3284 year: 1998 ident: B20 article-title: Activated microglial cells migrate towards sites of excitotoxic neuronal injury inside organotypic hippocampal slice cultures publication-title: Eur. J. Neurosci. doi: 10.1046/j.1460-9568.1998.00379.x contributor: fullname: Heppner – volume: 39 start-page: 3 year: 2013 ident: B6 article-title: Review: activation patterns of microglia and their identification in the human brain publication-title: Neuropathol. Appl. Neurobiol. doi: 10.1111/nan.12011 contributor: fullname: Boche – volume: 9 start-page: e97096 year: 2014 ident: B40 article-title: Rod microglia: a morphological definition publication-title: PLoS ONE doi: 10.1371/journal.pone.0097096 contributor: fullname: Taylor – volume: 11 start-page: 12 year: 2014 ident: B41 article-title: Morphometric characterization of microglial phenotypes in human cerebral cortex publication-title: J. Neuroinflammation doi: 10.1186/1742-2094-11-12 contributor: fullname: Torres-Platas – volume: 31 start-page: 366 year: 2012 ident: B1 article-title: Calcium chloride made E. coli competent for uptake of extraneous DNA through overproduction of OmpC protein publication-title: Protein J. doi: 10.1007/s10930-012-9411-z contributor: fullname: Aich – volume: 7 issue: 3 year: 2013 ident: B24 article-title: Quantitating the subtleties of microglial morphology with fractal analysis publication-title: Front. Cell. Neurosci. doi: 10.3389/fncel.2013.00003 contributor: fullname: Karperien – volume: 8 start-page: 271 year: 2010 ident: B3 article-title: The human immune response to Dengue virus is dominated by highly cross-reactive antibodies endowed with neutralizing and enhancing activity publication-title: Cell Host Microbe doi: 10.1016/j.chom.2010.08.007 contributor: fullname: Beltramello – volume: 22 start-page: 1442 year: 2012 ident: B21 article-title: Evidence that microglia mediate the neurobiological effects of chronic psychological stress on the medial prefrontal cortex publication-title: Cereb. Cortex doi: 10.1093/cercor/bhr229 contributor: fullname: Hinwood – volume: 168 start-page: 32 year: 2001 ident: B25 article-title: Effect of lipopolysaccharide on the morphology and integrin immunoreactivity of ramified microglia in the mouse brain and in cell culture publication-title: Exp. Neurol. doi: 10.1006/exnr.2000.7575 contributor: fullname: Kloss – volume: 519 start-page: 2803 year: 2011 ident: B8 article-title: Analysis of differential shrinkage in frozen brain sections and its implications for the use of guard zones in stereology publication-title: J. Comp. Neurol. doi: 10.1002/cne.22652 contributor: fullname: Carlo – volume: 85 start-page: 169 year: 1988 ident: B36 article-title: The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system publication-title: Neurosci. Lett. doi: 10.1016/0304-3940(88)90346-1 contributor: fullname: Shu – volume: 60 start-page: 421 year: 2003 ident: B17 article-title: Neutralization and antibody-dependent enhancement of dengue viruses publication-title: Adv. Virus Res. doi: 10.1016/S0065-3527(03)60011-4 contributor: fullname: Halstead – volume: 29 start-page: 3974 year: 2009 ident: B44 article-title: Resting microglia directly monitor the functional state of synapses in vivo and determine the fate of ischemic terminals publication-title: J. Neurosci. doi: 10.1523/JNEUROSCI.4363-08.2009 contributor: fullname: Wake – volume: 308 start-page: 1314 year: 2005 ident: B30 article-title: Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo publication-title: Science doi: 10.1126/science.1110647 contributor: fullname: Nimmerjahn – volume: 521 start-page: 1184 year: 2013 ident: B47 article-title: Novel objective classification of reactive microglia following hypoglossal axotomy using hierarchical cluster analysis publication-title: J. Comp. Neurol. doi: 10.1002/cne.23228 contributor: fullname: Yamada – volume: 220 start-page: 2423 year: 2015 ident: B31 article-title: Widespread activation of microglial cells in the hippocampus of chronic epileptic rats correlates only partially with neurodegeneration publication-title: Brain Struct. Funct. doi: 10.1007/s00429-014-0802-0 contributor: fullname: Papageorgiou – volume: 13 start-page: 719 year: 2013 ident: B13 article-title: Aging and environmental enrichment exacerbate inflammatory response on antibody-enhanced dengue disease in immunocompetent murine model publication-title: Eur. J. Inflam. doi: 10.1177/1721727X1301100315 contributor: fullname: Diniz – volume: 61–62 start-page: 176 year: 2014 ident: B33 article-title: Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer publication-title: J. Chem. Neuroanat. doi: 10.1016/j.jchemneu.2014.10.001 contributor: fullname: Santos-Filho – volume: 36 start-page: 3 year: 2016 ident: B43 article-title: Antibody-enhanced dengue disease generates a marked CNS inflammatory response in the black-tufted marmoset Callitrix penicillata publication-title: Neuropathology doi: 10.1111/neup.12229 contributor: fullname: Vasconcelos – volume: 225 start-page: 162 year: 2012 ident: B5 article-title: Microglial activation in the injured and healthy brain: what are we really talking about? Practical and theoretical issues associated with the measurement of changes in microglial morphology publication-title: Neuroscience doi: 10.1016/j.neuroscience.2012.07.029 contributor: fullname: Beynon – volume: 7 start-page: e38971 year: 2012 ident: B32 article-title: Dendritic morphology of hippocampal and amygdalar neurons in adolescent mice is resilient to genetic differences in stress reactivity publication-title: PLoS ONE doi: 10.1371/journal.pone.0038971 contributor: fullname: Pillai – volume: 162 start-page: 206 year: 2007 ident: B23 article-title: Design-based estimation of neuronal number and individual neuronal volume in the rat hippocampus publication-title: J. Neurosci. Methods doi: 10.1016/j.jneumeth.2007.01.009 contributor: fullname: Hosseini-Sharifabad – volume: 34 start-page: 73 year: 1994 ident: B11 article-title: Cellular forms and functions of brain microglia publication-title: Brain Res. Bull. doi: 10.1016/0361-9230(94)90189-9 contributor: fullname: Davis – volume: 1 start-page: 51 year: 2011 ident: B15 article-title: Cross-reactivity and expansion of dengue-specific T cells during acute primary and secondary infections in humans publication-title: Sci. Rep. doi: 10.1038/srep00051 contributor: fullname: Friberg – volume: 465 start-page: 161 year: 2003 ident: B34 article-title: Magic peptides, magic antibodies: guidelines for appropriate controls for immunohistochemistry publication-title: J. Comp. Neurol. doi: 10.1002/cne.10858 contributor: fullname: Saper – volume: 6 start-page: e1000790 year: 2010 ident: B2 article-title: Lethal antibody enhancement of dengue disease in mice is prevented by Fc modification publication-title: PLoS Pathog. doi: 10.1371/journal.ppat.1000790 contributor: fullname: Balsitis – volume: 1 start-page: 100 year: 1997 ident: B35 article-title: The use of cluster analysis for cell typing publication-title: Brain Res. Brain Res. Protoc. doi: 10.1016/S1385-299X(96)00014-1 contributor: fullname: Schweitzer – volume: 10 start-page: e1004031 year: 2014 ident: B29 article-title: First experimental in vivo model of enhanced dengue disease severity through maternally acquired heterotypic dengue antibodies publication-title: PLoS Pathog. doi: 10.1371/journal.ppat.1004031 contributor: fullname: Ng – volume: 29 start-page: 136 year: 2013 ident: B10 article-title: Microglial activation, increased TNF and SERT expression in the prefrontal cortex define stress-altered behaviour in mice susceptible to anhedonia publication-title: Brain Behav. Immun. doi: 10.1016/j.bbi.2012.12.017 contributor: fullname: Couch – volume: 42 start-page: 2036 year: 2015 ident: B12 article-title: Three-dimensional morphometric analysis of microglial changes in a mouse model of virus encephalitis: age and environmental influences publication-title: Eur. J. Neurosci. doi: 10.1111/ejn.12951 contributor: fullname: de Sousa – volume: 33 start-page: 256 year: 2001 ident: B38 article-title: Dynamics of microglial activation: a confocal time-lapse analysis in hippocampal slices publication-title: Glia doi: 10.1002/1098-1136(200103)33:3256::AID-GLIA10243.0.CO;2-J contributor: fullname: Stence – volume: 33 start-page: 191 year: 2012 ident: B19 article-title: Microglia in the developing brain: a potential target with lifetime effects publication-title: Neurotoxicology doi: 10.1016/j.neuro.2012.01.012 contributor: fullname: Harry – volume: 81 start-page: 1079 year: 2013 ident: B4 article-title: Microglia: multiple roles in surveillance, circuit shaping, and response to injury publication-title: Neurology doi: 10.1212/WNL.0b013e3182a4a577 contributor: fullname: Benarroch – volume: 107 start-page: 1021 year: 2012 ident: B14 article-title: Environmental influences on antibody-enhanced dengue disease outcomes publication-title: Mem. Inst. Oswaldo Cruz doi: 10.1590/S0074-02762012000800010 contributor: fullname: Diniz – volume: 23 start-page: 1784 year: 2013 ident: B22 article-title: Chronic stress induced remodeling of the prefrontal cortex: structural re-organization of microglia and the inhibitory effect of minocycline publication-title: Cereb. Cortex doi: 10.1093/cercor/bhs151 contributor: fullname: Hinwood – volume: 35 start-page: 4552 year: 2015 ident: B9 article-title: Selective activation of microglia facilitates synaptic strength publication-title: J. Neurosci. doi: 10.1523/JNEUROSCI.2061-14.2015 contributor: fullname: Clark – volume: 7 issue: 70 year: 2013 ident: B28 article-title: Microglia and synapse interactions: fine tuning neural circuits and candidate molecules publication-title: Front. Cell. Neurosci. doi: 10.3389/fncel.2013.00070 contributor: fullname: Miyamoto
SSID	ssj0062655
Score	2.1433368
Snippet	It is known that microglial morphology and function are related, but few studies have explored the subtleties of microglial morphological changes in response...
SourceID	doaj pubmedcentral proquest crossref pubmed
SourceType	Open Website Open Access Repository Aggregation Database Index Database
StartPage	23
SubjectTerms	Animals Antigens Biodiversity Brain research Callithrix penicillata Cluster analysis Dengue fever dengue virus infection Dentate Gyrus Encephalitis Genotypes hierarchical cluster analysis Immunoglobulins Infections Microglia microglial morphological subtleties Morphology Neuropathology Neuroscience Phenotypes Principal components analysis Serotypes Tumor necrosis factor-α Vectors
SummonAdditionalLinks	– databaseName: Directory of Open Access Journals dbid: DOA link: http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV09j9QwELXgKhoEHB-BAw0SQqKILnacOCmP405b0QASXeT4YzfSrnO6JAL-KT-HGWezYhESDU2KxEqcvPF4Xjx-w9ibQjkqRpOlqlR5Kq0taLNyTQckH7U2sqVfA6tP6uPX6sMVyeQcSn1RTtgsDzx_uHNv2pZ75XmBgYpWviqsEdYYlam2reWs85lVC5mafTBG6UUxL0oiBavPfdCBRIY4rTxkIj-ahKJW_98CzD_zJH-beK4fsPv7iBEu5p4-ZHdceMROLwKy5d0PeAsxhzP-HD9lP1cdbSiO9U22YLYTySCA3guPQO9hROxcaknTf9bjgEiJDzKysdEU2g5nNwuDJvFgCztK21tvOw3Dpv82QOhHoCT3Lkz9NMCuR7QWLwrzXuIBaOcKYPC5dsBh7EFALEgOSw4joMtbTw6WfDB8dhfgUm-RGWxuu-9w40JnqCrSqB-zL9dXny9X6b52Q2pknY2p4NYLLUpkM-jGaqWlapGbuRYjNKcl8hhdVV6Tep8Upi3bvDRaG-W1yXnuRf6EnYQ-uGcMeN0qZTNpsKms8PZSl1ZzLzGY8jjVJOzdAmZzM0t0NEhtCPgmAt8Q8E0EPmHvCe1DOxLXjifQ5Jq9yTX_MrmEnS220uxH_NBQWXSMRZHgJez14TKOVVqA0cEhGg1X6P24LHKVsKezaR16IlQmVS6LhKkjozvq6vGV0G2iHjgpAFUlf_4_3u0Fu0dfi7LshDhjJ2h97iW7O9jpVRxhvwDpyDQZ priority: 102 providerName: Directory of Open Access Journals
Title	Hierarchical Cluster Analysis of Three-Dimensional Reconstructions of Unbiased Sampled Microglia Shows not Continuous Morphological Changes from Stage 1 to 2 after Multiple Dengue Infections in Callithrix penicillata
URI	https://www.ncbi.nlm.nih.gov/pubmed/27047345 https://www.proquest.com/docview/2295561174 https://search.proquest.com/docview/1779414537 https://pubmed.ncbi.nlm.nih.gov/PMC4801861 https://doaj.org/article/fcbb1f7f15774a7f85dc2dcc707bb943
Volume	10
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nj9MwELXonrggYPnosqwGCSFxyLZJnDg5Lu2uyqEIqbsSt8h27DZS6lTbVsA_5ecw4yRlizhxyaFxEysz45lnz7xh7H0iDDWjGQciFXHAyzKhYuWcLgg-cqm5oq2B2UJ8-ZZNr4kmJ-lrYXzSvlbVpavXl65a-dzKzVqP-jyx0df5hChPsjQcDdgAY8MeorfLLwboSdKeRyL6ykfWSUf8QiEdOqCDIvZfMeYipgKmB67IM_b_K8z8O1vygfu5ecqedHEjXLXze8YeGfecnV45xMzrn_ABfCan3yI_Zb9mFZUV-y4nNUzqPZEhQE8_Ao2FW5SgCabE7N-ycgDB0D9ksn7QnVMV-rgSFpIohEuYU_Lesq4kLFbN9y24ZgfEblW5fbPfwrxBmfVrKbRlC1ug-hXAkHZpIIRdAxH4tuQw7zIZYWrccm_gc5cVhu-uHExkjfhgdV_9gI1xlabeSDv5gt3dXN9OZkHXwSHQPB_vgigsbSSjFDENLma5kFwoRGhGYZxmJEc0I7PMSuLw45FWqYpTLaUWVuo4jG0Uv2QnrnHmNYMwV0KUY65xKM_w8VympQwtx5DKosMZso-9MItNS9RRIMAhHSi8DhSkA4XXgSH7RNI-jCOKbf9Dc78sOkUrrFYqtMKGCUbIUtgsKXVUai3GQqmc40POe10pOrvfFtQcHSNShHlD9u5wGy2WjmGkMyiNIhS4BoY8icWQvWpV6zCTXjWHTBwp3dFUj--gkXhW8M4ozv77n2_YY_pElGAXRefsBFXOvGWDbbm_8DsVF97OfgPh-TI6
link.rule.ids	230,315,729,782,786,866,887,2108,27935,27936,53803,53805
linkProvider	National Library of Medicine
linkToHtml	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwELbY5QAXXsujsMAgISQO2ebhxMlxaXfVFdsVUrsSt8h2nDZS61TbVsA_5ecw4yRlizjtpYfYSa3483i-eOYbxj7GwlAxGt8TiYg8XhQxJStn9IPkI5OaK_o0MJqIq-_p8IxkcuIuF8YF7WtVndjF8sRWcxdbuVrqfhcn1v82HpDkSZoE_QN2H9erH3UkvTHA6KLHcXMiifwr65dWWlIYCujYAbco0v8VPhcRpTDd2oycZv__HM1_4yVvbUDnj-849CfsUetxwmnT_JTdM_YZOzq1yLaXv-ATuBhQ93H9iP0eVZSQ7OqjLGCw2JKMAnTCJVCXMMW5N96QagI0eh5ABPavDK3rdG1VhbtjARNJ4sMFjCnsb7aoJEzm9Y812HoDpItV2W29XcO4xtnurDA0CQ9roMwXQGd4ZiCATQ0huILmMG5jIGFo7Gxr4KKNJ8P_riwM5AKZxfym-gkrYytNVZU28jm7Pj-bDkZeW_vB0zzzN14YFGUowwTZEJrBTEguFHI7o9DDM5IjD5JpWkpS_-OhVomKEi2lFqXUURCVYfSCHdramlcMgkwJUfhcY1ee4uO5TAoZlBydsRK3qh773IEgXzUSHzlSI8JO7rCTE3Zyh50e-0Io2fUjcW53ob6Z5e0856VWKihFGcToW0tRpnGhw0Jr4QulMo4POe4wlrcWY51TWXX0ZZEg9tiHXTOudTrAkdbgbOSBQOsZ8DgSPfaygeRuJB2ke0zsgXVvqPstiFGnJ95i8vWd73zPHoym48v88uLq6xv2kF4XhemF4TE7RPiZt-xgXWzfuVX6BwB3Rsw
linkToPdf	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lj9MwELbYRUJceC2PwgKDhJA4ZJuHEyfHpd2qK-hqpe5K3CLHsdtIrVNtWwH_lJ_DjJOULeIElx4aN7XizzPzxTPfMPY-Fpqa0fieSETk8bKMqVg5ow8kH5lUvKBXA-OpuPiaDs9IJmfX6ssl7auiOrGL5Ymt5i63crVU_S5PrH85GZDkSZoE_VVp-gfsLu5ZP-6IemOEMUyP4-ZUEjlY1jdWWlIZCujoAd0UaQALn4uIyphuOSSn2_-3YPPPnMlbTmj08D-m_4g9aCNPOG2GPGZ3tH3Cjk4tsu7lD_gALhfUvWQ_Yj_HFRUmuz4pCxgstiSnAJ2ACdQGrhAD2htSb4BG1wOIyP6Wo3WDrm1RoZcsYSpJhLiECaX_zRaVhOm8_rYGW2-A9LEqu623a5jUuOqdNYam8GENVAEDGBTPNASwqSEE19gcJm0uJAy1nW01nLd5ZfjflYWBXCDDmN9U32GlbaWou9JGPmXXo7Orwdhre0B4imf-xguD0oQyTJAVoTnMhOSiQI6nC4z0tOTIh2SaGkkqgDxURVJEiZJSCSNVFEQmjJ6xQ1tb_YJBkBVClD5XOJSneHsuk1IGhmNQZtBl9djHDgj5qpH6yJEiEX5yh5-c8JM7_PTYJ0LKbhyJdLsv6ptZ3q51blRRBEaYIMYYWwqTxqUKS6WEL4oi43iT4w5neWs51jm1V8eYFolij73bXcY9Twc50mpcjTwQaEUDHkeix543sNzNpIN1j4k9wO5Ndf8K4tTpire4fPnPv3zL7l0OR_mX84vPr9h9elqUrReGx-wQ0adfs4N1uX3jNuov-a9JTA
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Hierarchical+Cluster+Analysis+of+Three-Dimensional+Reconstructions+of+Unbiased+Sampled+Microglia+Shows+not+Continuous+Morphological+Changes+from+Stage+1+to+2+after+Multiple+Dengue+Infections+in+Callithrix+penicillata&rft.jtitle=Frontiers+in+neuroanatomy&rft.au=Diniz%2C+Daniel+G&rft.au=Silva%2C+Geane+O&rft.au=Naves%2C+Tha%C3%ADs+B&rft.au=Fernandes%2C+Taiany+N&rft.date=2016-03-22&rft.pub=Frontiers+Research+Foundation&rft.eissn=1662-5129&rft_id=info:doi/10.3389%2Ffnana.2016.00023&rft.externalDBID=HAS_PDF_LINK
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1662-5129&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1662-5129&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1662-5129&client=summon