Characterization of multidrug-resistant potential pathogens isolated from milk and some dairy products in Egypt

• Published in: Journal of advanced veterinary and animal research Vol. 10; no. 2; pp. 275 - 283
• Main Authors: Ashraf, Dina, Ombarak, Rabee A, Samir, Ahmed, Abdel-Salam, Ayah B
• Format: Journal Article
• Language: English
• Published: Bangladesh Network for the Veterinarians of Bangladesh Bangladesh Agricultural Universityת Faculty of Veterinary Science 01-06-2023; A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET); Network for the Veterinarians of Bangladesh
• Subjects: Amoxicillin; Ampicillin; Antibiotics; Antimicrobial agents; Antimicrobial resistance; antimicrobial resistance; dairy products; potential pathogens; public health; Bacillus cereus; Bacteria; Cefotaxime; Cheese; Dairy products; Drug resistance; E coli; Food contamination & poisoning; Gastrointestinal diseases; Genes; Gentamicin; Health hazards; Microorganisms; Milk; Multidrug resistance; Original; Pasteurization; Pathogens; Public health; Rice; Staphylococcus aureus; Strains (organisms); β-Lactam antibiotics; United Kingdom > UK; Egypt; potential pathogens; Antimicrobial resistance; dairy products; public health
• ISSN: 2311-7710; 2311-7710
• DOI: 10.5455/javar.2023.j679

This study aimed to explore the incidence and antimicrobial resistance (AMR) of
,
, and
in raw milk and some Egyptian dairy products, namely Kariesh cheese and rice with milk.
112 samples (70 raw milk, 30 Kariesh cheese, and 12 rice with milk) were randomly collected from different districts in Cairo and Giza, Egypt. The samples were examined for
,
, and
presence. The susceptibility of the obtained isolates was tested against 11 antimicrobials using the disk diffusion method, and further, the presence of AMR genes was examined.
The incidences of
,
, and
were 69.64%, 12.5%, and 16.7% in the examined samples, respectively
The antibiogram indicated that
isolates (
60) were resistant to gentamycin (73.33%), ampicillin (AM, 53.3%), and cefotaxime (CTX, 16.66%). Multidrug-resistant (MDR)
strains (
5) were tested for β-lactams resistance genes.
was detected in all isolates, and two of them additionally carried
.
isolates (
10) were resistant to AM (100%), followed by tetracycline (TE), CTX, and gentamycin (60% each). All MDR
strains (
4) carried
Z and
, and three of them additionally carried
(6')-
(2'').
isolates (
30) showed resistance to AM (100%), amoxicillin (20%), and TE (6.66%).
and
A genes were detected in all MDR
isolates (
6).
Our findings denote the high incidence of potential health hazards in raw milk and some of its products and the existence of AMR bacteria, including MDR strains, which can cause human illnesses that are difficult to treat.