Submerged cultivation of Ganoderma lucidum biomass and immunostimulatory effects of fungal polysaccharides
Original Ganoderma lucidum strain MZKI G97 isolated from Slovenian forests was cultivated in a liquid substrate based on potato dextrose and olive oil. The influences of inoculum and oxygen partial pressure in batch and fed batch cultivation in a 10-l laboratory stirred tank reactor were studied. Fu...
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Published in: | Journal of biotechnology Vol. 103; no. 1; pp. 77 - 86 |
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Main Authors: | , , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Lausanne
Elsevier B.V
12-06-2003
Amsterdam Elsevier New York, NY |
Subjects: | |
Online Access: | Get full text |
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Summary: | Original
Ganoderma lucidum strain MZKI G97 isolated from Slovenian forests was cultivated in a liquid substrate based on potato dextrose and olive oil. The influences of inoculum and oxygen partial pressure in batch and fed batch cultivation in a 10-l laboratory stirred tank reactor were studied. Fungal biomass was found to be oxygen and shear sensible. Using a 17% (wet weight) 6 days old vegetative inoculum, 9.6 g l
−1 of dry biomass in batch cultivation and 15.2 g l
−1 in fed batch process were obtained. Extracellular (9.6 g l
−1) and intracellular (6.3 g l
−1) polysaccharide fractions were isolated. Extracellular polysaccharide fraction and four intracellular polysaccharide fractions were obtained. Polysaccharides were further separated by ion-exchange, gel and affinity chromatography. The isolated polysaccharides were mainly β-
d-glucanes. Immunostimulatory effects of isolates were tested on induction of cytokine (tumour necrosis factor α (TNF-α) and interferon γ (IFN-γ)) synthesis in primary cultures of human peripheral blood mononuclear cells (PBMC) isolated from a buffy coat. The TNF-α inducing activity is comparable with romurtide, which has been used as a supporting therapy in cancer patients treated with radiotherapy and/or chemotherapy. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0168-1656 1873-4863 |
DOI: | 10.1016/S0168-1656(03)00069-5 |