Simultaneous determination of methamphetamine and its metabolite, amphetamine, in urine using a high performance liquid chromatography column-switching method

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 845; no. 1; pp. 180 - 183
• Main Authors: Kumihashi, Mitsuru, Ameno, Kiyoshi, Shibayama, Takayuki, Suga, Keisuke, Miyauchi, Hiroshi, Jamal, Mostofa, Wang, Weihuan, Uekita, Ikuo, Ijiri, Iwao
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 2007; Elsevier Science
• Subjects: Amphetamine; Amphetamine - urine; Amphetamine-Related Disorders - urine; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Column-switching; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC; Hydrogen-Ion Concentration; Medical sciences; Methamphetamine; Methamphetamine - urine; Pharmacology. Drug treatments; Spectrophotometry, Ultraviolet; Substance Abuse Detection - methods; Urine; Urine; Amphetamine; Column-switching; HPLC; Methamphetamine; Amphetamine derivatives; Biological fluid; Chemical analysis; Column switching; CNS stimulant; Metabolite; Psychotropic; Metamfetamine; HPLC chromatography; Sympathomimetic; Simultaneous measurement; Amfetamine; Column chromatography
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2006.07.049

We describe here a simple, precise, and highly sensitive method for the simultaneous determination of methamphetamine (MA) and amphetamine (AM) in urine using a high performance liquid chromatography (HPLC) column-switching method. A PK-2A (Shodex) column was used for extraction and deproteinization, and a CAPCELL PAK SCX semi-micro, polymer-coated cation-exchange column was employed for separation. The urine sample was mixed with an equal volume of borate buffer (0.1
M, pH 9.4), and then 100
μl of the mixture was injected into the HPLC column. The column was switched for 6
min, and then 10
min later detection was performed at 210
nm. Recovery yields of the MA and AM spiked in the urine were 93.0–100.4% with a coefficient of variation of less than 1%. The calibration curves of MA and AM were in the range of 0.1–10
μg/ml with good linearity (
r
2
=
0.999), with the limit of qualification being 0.005
μg/ml. This method of using HPLC with column-switching can be used for both qualification and quantification of MA and its metabolite, AM, in urine, especially in forensic cases.