Miniaturized hollow fiber assisted liquid-phase microextraction and gas chromatography–mass spectrometry for determination of benzophenone and derivates in human urine sample

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 877; no. 3; pp. 298 - 302
• Main Authors: Kawaguchi, Migaku, Ito, Rie, Honda, Hidehiro, Koganei, Youji, Okanouchi, Noriya, Saito, Koichi, Seto, Yasuo, Nakazawa, Hiroyuki
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-01-2009; Elsevier
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Benzophenone (BP); Benzophenones - metabolism; Benzophenones - urine; Biological and medical sciences; Chemical Fractionation - methods; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry - methods; GC–MS; General pharmacology; Hollow fiber (HF); Humans; Liquid-phase microextraction (LPME); Medical sciences; Miniaturization; Miniaturized; Pharmacology. Drug treatments; Photosensitizing Agents - metabolism; Photosensitizing Agents - urine; Reproducibility of Results; Sensitivity and Specificity; Solvents - chemistry; Time Factors; Liquid-phase microextraction (LPME); GC–MS; Hollow fiber (HF); Benzophenone (BP); Miniaturized; Human; Urine; Biological fluid; Benzophenone derivatives; Liquid liquid extraction; Determination; Liquid phase microextraction; Chemical enrichment; GC-MS; Hydrofluoric acid; Hollow fiber; Miniaturization; Mass spectrometry; Quantitative analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2008.12.021

The determination of benzophenones (BPs) in human urine sample by miniaturized hollow fiber assisted liquid-phase microextraction (HF-LPME) and gas chromatography–mass spectrometry (GC–MS) is described. As analytes, BP, its metabolites benzhydrol (BP-OH) and 2-hydroxybenzophenone (2OH-BP), and its derivatives 2-hydroxy-4-methoxybenzophenone (BP-3) and 2-hydroxy-4-methoxy-4′-methylbenzophenone (BP-10) were selected. The detection limit and the quantification limit of BPs in human urine sample are 5–10 and 20–50
pg
mL
−1, respectively. The calibration curve for BPs is linear with correlation coefficient higher than 0.99 in the range of 0.02–10 or 0.05–10
ng
mL
−1. The average recoveries of BPs in human urine samples spiked with 0.5 and 5
ng
mL
−1 BPs are 89.8–100.2% (RSD: 2.5–9.3%) and 89.3–99.9% (RSD: 2.9–3.7%), respectively. Ten human urine samples were analyzed using the present method. BP-OH and BP-3 were detected in all the samples within the range of 0.24–5.91 and 0.43–5.17
ng
mL
−1, respectively. This simple, sensitive, and selective analytical method was successfully applied to the determination of trace amounts of BPs in human urine samples.