Aeromonas molluscorum sp. nov., isolated from bivalve molluscs

• Published in: International journal of systematic and evolutionary microbiology Vol. 54; no. 6; pp. 2073 - 2078
• Main Authors: Minana-Galbis, David, Farfan, Maribel, Fuste, M. Carme, Loren, J. Gaspar
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-11-2004; Society for General Microbiology
• Subjects: Aeromonas - chemistry; Aeromonas - classification; Aeromonas - isolation & purification; Aeromonas - physiology; Aeromonas encheleia; Aeromonas molluscorum; Animals; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Bivalvia; Carboxy-Lyases - analysis; DNA Fingerprinting; DNA, Bacterial - chemistry; DNA, Bacterial - isolation & purification; DNA, Ribosomal - chemistry; DNA, Ribosomal - isolation & purification; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Glucose - metabolism; Indoles - metabolism; Marine; Microbiology; Miscellaneous; Molecular Sequence Data; Mollusca; Mollusca - microbiology; Nucleic Acid Hybridization; Phylogeny; Polymorphism, Genetic; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Starch - metabolism; Systematics; Marine environment; Bivalvia; Taxonomy; Aeromonas; Bacteria; Vibrionaceae; New species; Invertebrata; Mollusca
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63202-0

Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain
Correspondence J. Gaspar Lorén jgloren{at}ub.edu
Five Aeromonas strains (848T T , 93M, 431E, 849T and 869N), which were isolated from bivalve molluscs and were recognized previously by numerical taxonomy as members of an unknown Aeromonas taxon, were subjected to a polyphasic taxonomic study. DNA–DNA hybridization experiments showed that DNA of strain 848T T was <70 % similar (27–45 %) to that of the type/reference strains of the current Aeromonas hybridization groups (HGs), but 93 % similar to that of strain 93M. The DNA G+C content of the five strains ranged from 59·0 to 59·4 mol%. 16S rRNA gene sequence analysis confirmed that the strains belonged to the genus Aeromonas and showed high similarity to Aeromonas encheleia . Amplified fragment length polymorphism fingerprinting clustered the novel strains in a homogeneous group with low genotypic relatedness to other Aeromonas species. Useful phenotypic features for differentiating the five isolates from other Aeromonas species include their negative reactions in tests for indole production, lysine decarboxylase, gas from glucose and starch hydrolysis. From the results of this study, the name Aeromonas molluscorum sp. nov. is proposed for these strains, with the type strain 848T T (=CECT 5864 T =LMG 22214 T ).
Abbreviations: ADH, arginine dehydrolase; AFLP, amplified fragment length polymorphism; HG, hybridization group; LDC, lysine decarboxylase; MR, methyl red; ODC, ornithine decarboxylase; VP, Voges–Proskauer
Published online ahead of print on 15 June 2004 as DOI 10.1099/ijs.0.63202-0.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 93M, 431E, 848T T , 849T and 869N are AY532688 – AY532692 , respectively.
A dendrogram of AFLP profiles and tables showing 16S rRNA gene sequence similarities and DNA–DNA hybridization data are available as supplementary material in IJSEM Online.