Fabrication of plasmon length-based surface enhanced Raman scattering for multiplex detection on microfluidic device

The length of bioreceptors plays an important role in signal enhancement of surface-enhanced Raman scattering (SERS) due to amplification of electromagnetic fields generated by the excitation of localized surface plasmons. Herein, intact antibodies (IgG) and Fab fragments conjugated onto gold nanost...

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Published in:	Biosensors & bioelectronics Vol. 70; pp. 358 - 365
Main Authors:	Nguyen, Anh H., Lee, Jeewon, Il Choi, Hong, Seok Kwak, Ho, Jun Sim, Sang
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 15-08-2015
Subjects:	Animals Antigens Biomarkers, Tumor - analysis Biomarkers, Tumor - immunology Cancer biomarkers Complex Mixtures - immunology Deposition Devices Equipment Design Equipment Failure Analysis Humans Immunoassay - instrumentation Immunoglobulin Fab Fragments - immunology Immunoglobulin G - immunology Immunosensors Lab-On-A-Chip Devices Microarray Analysis - instrumentation Microfluidics Multiplex detection Multiplexing Plasmonic nanoparticles Raman scattering Reproducibility of Results Sensitivity and Specificity SERS Silver Surface Plasmon Resonance - instrumentation Cancer biomarkers SERS Multiplex detection Microfluidics Plasmonic nanoparticles
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Abstract	The length of bioreceptors plays an important role in signal enhancement of surface-enhanced Raman scattering (SERS) due to amplification of electromagnetic fields generated by the excitation of localized surface plasmons. Herein, intact antibodies (IgG) and Fab fragments conjugated onto gold nanostar were used to fabricate two kinds of immunosensors for measurement of their SERS signals. Using CA125 as the antigen and Rhodamine-6G (R6G)-conjugated immunogolds, a SERS immunosensor was self-assembled by antigen-antibody interaction. The results showed that the SERS signal from the Fab immunosensor was 2.4 times higher than that of the IgG immunosensor. Furthermore, increased hot-spots by silver atom deposition onto the IgG and Fab immunosensor showed 2.1 and 1.4 times higher signals than before enhancement, respectively. For application, based on the Fab immunosensor, a SERS-compatible microfluidic system was designed for multiplex assays to overcome the drawbacks of conventional assays. This system can measure biological specimens directly from bio fluids instead of using a complex microfluidic device containing separation and detection elements. Four approved biomarkers of breast cancer, including cancer antigen (CA125), HER2, epididymis protein (HE4), and Eotaxin-1, were detected from patient-mimicked serum with limits of 15fM, 17fM, 21fM, and 6.5fM, respectively. The results indicated that the lengths and geometry of the bioreceptors determined the intensity of SERS signal from the interface and cavity of the sandwich immunosensor. Silver atom deposition at the cavity of the immunosensor increased the SERS signal. Finally, the SERS immunosensor built-in microfluidic system improved the performance of multiplex diagnostics. •Fabrication of plasmon length based SERS immunosensor based on IgG and Fab molecules.•Fab immunogold array showed sensitivity in integrated SERS-microfluidics device.•Increased hot spots numbers on the immunosensor surface in microfluidic device.•Detection for a panel of breast cancer biomarkers at ultralow concentration.
AbstractList	The length of bioreceptors plays an important role in signal enhancement of surface-enhanced Raman scattering (SERS) due to amplification of electromagnetic fields generated by the excitation of localized surface plasmons. Herein, intact antibodies (IgG) and Fab fragments conjugated onto gold nanostar were used to fabricate two kinds of immunosensors for measurement of their SERS signals. Using CA125 as the antigen and Rhodamine-6G (R6G)-conjugated immunogolds, a SERS immunosensor was self-assembled by antigen-antibody interaction. The results showed that the SERS signal from the Fab immunosensor was 2.4 times higher than that of the IgG immunosensor. Furthermore, increased hot-spots by silver atom deposition onto the IgG and Fab immunosensor showed 2.1 and 1.4 times higher signals than before enhancement, respectively. For application, based on the Fab immunosensor, a SERS-compatible microfluidic system was designed for multiplex assays to overcome the drawbacks of conventional assays. This system can measure biological specimens directly from bio fluids instead of using a complex microfluidic device containing separation and detection elements. Four approved biomarkers of breast cancer, including cancer antigen (CA125), HER2, epididymis protein (HE4), and Eotaxin-1, were detected from patient-mimicked serum with limits of 15fM, 17fM, 21fM, and 6.5fM, respectively. The results indicated that the lengths and geometry of the bioreceptors determined the intensity of SERS signal from the interface and cavity of the sandwich immunosensor. Silver atom deposition at the cavity of the immunosensor increased the SERS signal. Finally, the SERS immunosensor built-in microfluidic system improved the performance of multiplex diagnostics. The length of bioreceptors plays an important role in signal enhancement of surface-enhanced Raman scattering (SERS) due to amplification of electromagnetic fields generated by the excitation of localized surface plasmons. Herein, intact antibodies (IgG) and Fab fragments conjugated onto gold nanostar were used to fabricate two kinds of immunosensors for measurement of their SERS signals. Using CA125 as the antigen and Rhodamine-6G (R6G)-conjugated immunogolds, a SERS immunosensor was self-assembled by antigen-antibody interaction. The results showed that the SERS signal from the Fab immunosensor was 2.4 times higher than that of the IgG immunosensor. Furthermore, increased hot-spots by silver atom deposition onto the IgG and Fab immunosensor showed 2.1 and 1.4 times higher signals than before enhancement, respectively. For application, based on the Fab immunosensor, a SERS-compatible microfluidic system was designed for multiplex assays to overcome the drawbacks of conventional assays. This system can measure biological specimens directly from bio fluids instead of using a complex microfluidic device containing separation and detection elements. Four approved biomarkers of breast cancer, including cancer antigen (CA125), HER2, epididymis protein (HE4), and Eotaxin-1, were detected from patient-mimicked serum with limits of 15 fM, 17 fM, 21 fM, and 6.5 fM, respectively. The results indicated that the lengths and geometry of the bioreceptors determined the intensity of SERS signal from the interface and cavity of the sandwich immunosensor. Silver atom deposition at the cavity of the immunosensor increased the SERS signal. Finally, the SERS immunosensor built-in microfluidic system improved the performance of multiplex diagnostics. The length of bioreceptors plays an important role in signal enhancement of surface-enhanced Raman scattering (SERS) due to amplification of electromagnetic fields generated by the excitation of localized surface plasmons. Herein, intact antibodies (IgG) and Fab fragments conjugated onto gold nanostar were used to fabricate two kinds of immunosensors for measurement of their SERS signals. Using CA125 as the antigen and Rhodamine-6G (R6G)-conjugated immunogolds, a SERS immunosensor was self-assembled by antigen-antibody interaction. The results showed that the SERS signal from the Fab immunosensor was 2.4 times higher than that of the IgG immunosensor. Furthermore, increased hot-spots by silver atom deposition onto the IgG and Fab immunosensor showed 2.1 and 1.4 times higher signals than before enhancement, respectively. For application, based on the Fab immunosensor, a SERS-compatible microfluidic system was designed for multiplex assays to overcome the drawbacks of conventional assays. This system can measure biological specimens directly from bio fluids instead of using a complex microfluidic device containing separation and detection elements. Four approved biomarkers of breast cancer, including cancer antigen (CA125), HER2, epididymis protein (HE4), and Eotaxin-1, were detected from patient-mimicked serum with limits of 15fM, 17fM, 21fM, and 6.5fM, respectively. The results indicated that the lengths and geometry of the bioreceptors determined the intensity of SERS signal from the interface and cavity of the sandwich immunosensor. Silver atom deposition at the cavity of the immunosensor increased the SERS signal. Finally, the SERS immunosensor built-in microfluidic system improved the performance of multiplex diagnostics. •Fabrication of plasmon length based SERS immunosensor based on IgG and Fab molecules.•Fab immunogold array showed sensitivity in integrated SERS-microfluidics device.•Increased hot spots numbers on the immunosensor surface in microfluidic device.•Detection for a panel of breast cancer biomarkers at ultralow concentration.
Author	Jun Sim, Sang Lee, Jeewon Seok Kwak, Ho Nguyen, Anh H. Il Choi, Hong
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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/25841120$$D View this record in MEDLINE/PubMed
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Cites_doi	10.1021/la302795r 10.1021/jp0610867 10.1021/cg7009644 10.1021/jp8054747 10.1146/annurev.physchem.040808.090434 10.1038/12898 10.1021/jp0687908 10.1039/c3cs35515b 10.1038/nrc2212 10.1021/nl101454a 10.1016/S1369-7021(12)70017-2 10.1002/cphc.200800349 10.1038/nbt927 10.1038/nprot.2011.304 10.1006/meth.1999.0925 10.1002/smll.200800337 10.1038/nbt.2108 10.1021/nl049670j 10.1038/nature05058 10.1021/jp100669q 10.2147/NSA.S3788 10.1038/nm.2408 10.1021/nl051592s 10.1021/nl071008a 10.1016/S0167-9317(02)00494-X 10.1038/nbt1100 10.1039/C4CC03734K 10.1016/j.bios.2014.12.015 10.1021/jz300426p 10.1039/c3nr02924g 10.1073/pnas.0504917102 10.1063/1.3554315 10.1126/science.1159499 10.1038/ncomms1674
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Keywords	Cancer biomarkers SERS Multiplex detection Microfluidics Plasmonic nanoparticles
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References	Reinhard, Siu, Agarwal, Alivisatos, Liphardt (bib28) 2005; 5 Stockman (bib33) 2011; 64 Whitesides (bib37) 2006; 442 Nguyen, Ma, Sim (bib25) 2015; 66 Chrimes, Khoshmanesh, Stoddart, Mitchella, Zadeha (bib9) 2013; 42 Jensen, Schatz (bib18) 2006; 110 Alonso-González, Albella, Schnell, Chen, Huth, García-Etxarri, Casanova, Golmar, Arzubiaga, Hueso, Aizpurua, Hillenbrand (bib3) 2012; 3 Backmann, Zahnd, Huber, Bietsch, Plückthun, Lang, Güntherodt, Hegner, Gerber (bib5) 2005; 102 Ma, Yin, Xu, Wu, Kuang, Wang, Xu (bib23) 2014; 50 Sokolov, Follen, Aaron, Pavlova, Malpica, Lotan, Richards-Kortum (bib32) 2003; 63 Wei, Xu (bib36) 2013; 5 Goh, Lee, Pedireddy, Phang, Tjiu, Tan, Ling (bib14) 2012; 28 Alivisatos (bib2) 2004; 22 Cai, Gao, Hong, Sun (bib7) 2008; 1 Stingl, Caldas (bib34) 2007; 10 Chin, Laksanasopin, Cheung, Steinmiller, Linder, Parsa, Wang, Moore, Rouse, Umviligihozo, Karita, Mwambarangwe, Braunstein, Wijgert, Sahabo, Justman, El-Sadr, Sia (bib8) 2011; 17 Sharma, Frontiera, Henry, Ringe, Van Duyne (bib30) 2012; 15 Fang, Seong, Dlott (bib11) 2008; 321 Shen, Xu, Liu, Raab, Bhakta, Kenrick, Parsons-Reponte, Tien, Yu, Mai, Li, Tibbitts, Baudys, Saad, Scales, McDonald, Hass, Eigenbrot, Nguyen, Solis, Fuji, Flagella, Patel, Spencer, Khawli, Ebens, Wong, Vandlen, Kaur, Sliwkowski, Scheller, Polakis, Junutula (bib31) 2012; 30 Le Ru, Blackie, Meyer, Etchegoin (bib21) 2007; 111 Raab, Han, Badt, Smith-Gill, Lindsay, Schindler, Hinterdorfer (bib27) 1999; 17 Franke, Wixforth (bib12) 2008; 9 Bogart, Pourroy, Murphy, Puntes, Pellegrino, Rosenblum, Peer, Lévy (bib6) 2014; 8 Meyer, Le Ru, Etchegoin (bib24) 2010; 114 Pérez, Moiraghi, Coronado, Macagno (bib26) 2008; 8 Dixit, Vashist, MacCraith, O’Kennedy. (bib10) 2011; 6 Ko, Singamaneni, Vladimir, Tsukruk (bib20) 2008; 4 Haes, Hall, Chang, Klein, Van Duyne (bib15) 2004; 4 Jain, Huang, El-Sayed (bib17) 2007; 7 Lu, Rycenga, Skrabalak, Wiley, Xia (bib22) 2009; 60 Fujii (bib13) 2002; 61 Sönnichsen, Reinhard, Liphardt, Alivisatos (bib35) 2005; 23 Adamczyk, Moore, Yu (bib1) 2000; 20 Ba, Rodríguez-Fernández, Stefani, Feldmann (bib4) 2010; 10 Khoury, Vo-Dinh (bib19) 2008; 112 Ringe, Langille, Sohn, Zhang, Huang, Mirkin, Van Duyne, Marks (bib29) 2012; 3 Jensen (10.1016/j.bios.2015.03.064_bib18) 2006; 110 Ringe (10.1016/j.bios.2015.03.064_bib29) 2012; 3 Reinhard (10.1016/j.bios.2015.03.064_bib28) 2005; 5 Khoury (10.1016/j.bios.2015.03.064_bib19) 2008; 112 Bogart (10.1016/j.bios.2015.03.064_bib6) 2014; 8 Chrimes (10.1016/j.bios.2015.03.064_bib9) 2013; 42 Nguyen (10.1016/j.bios.2015.03.064_bib25) 2015; 66 Sharma (10.1016/j.bios.2015.03.064_bib30) 2012; 15 Alivisatos (10.1016/j.bios.2015.03.064_bib2) 2004; 22 Goh (10.1016/j.bios.2015.03.064_bib14) 2012; 28 Fujii (10.1016/j.bios.2015.03.064_bib13) 2002; 61 Raab (10.1016/j.bios.2015.03.064_bib27) 1999; 17 Pérez (10.1016/j.bios.2015.03.064_bib26) 2008; 8 Franke (10.1016/j.bios.2015.03.064_bib12) 2008; 9 Stingl (10.1016/j.bios.2015.03.064_bib34) 2007; 10 Ko (10.1016/j.bios.2015.03.064_bib20) 2008; 4 Chin (10.1016/j.bios.2015.03.064_bib8) 2011; 17 Alonso-González (10.1016/j.bios.2015.03.064_bib3) 2012; 3 Sönnichsen (10.1016/j.bios.2015.03.064_bib35) 2005; 23 Ba (10.1016/j.bios.2015.03.064_bib4) 2010; 10 Le Ru (10.1016/j.bios.2015.03.064_bib21) 2007; 111 Whitesides (10.1016/j.bios.2015.03.064_bib37) 2006; 442 Dixit (10.1016/j.bios.2015.03.064_bib10) 2011; 6 Stockman (10.1016/j.bios.2015.03.064_bib33) 2011; 64 Fang (10.1016/j.bios.2015.03.064_bib11) 2008; 321 Wei (10.1016/j.bios.2015.03.064_bib36) 2013; 5 Lu (10.1016/j.bios.2015.03.064_bib22) 2009; 60 Jain (10.1016/j.bios.2015.03.064_bib17) 2007; 7 Sokolov (10.1016/j.bios.2015.03.064_bib32) 2003; 63 Backmann (10.1016/j.bios.2015.03.064_bib5) 2005; 102 Haes (10.1016/j.bios.2015.03.064_bib15) 2004; 4 Adamczyk (10.1016/j.bios.2015.03.064_bib1) 2000; 20 Meyer (10.1016/j.bios.2015.03.064_bib24) 2010; 114 Shen (10.1016/j.bios.2015.03.064_bib31) 2012; 30 Cai (10.1016/j.bios.2015.03.064_bib7) 2008; 1 Ma (10.1016/j.bios.2015.03.064_bib23) 2014; 50
References_xml	– volume: 9 start-page: 2140 year: 2008 end-page: 2156 ident: bib12 publication-title: Chem. Phys.Chem. contributor: fullname: Wixforth – volume: 50 start-page: 9737 year: 2014 end-page: 9740 ident: bib23 publication-title: Chem. Commun. contributor: fullname: Xu – volume: 6 start-page: 439 year: 2011 end-page: 445 ident: bib10 publication-title: Nat. Protoc. contributor: fullname: O’Kennedy. – volume: 5 start-page: 10794 year: 2013 end-page: 10805 ident: bib36 publication-title: Nanoscale contributor: fullname: Xu – volume: 3 start-page: 1479 year: 2012 end-page: 1483 ident: bib29 publication-title: J. Phys. Chem. Lett. contributor: fullname: Marks – volume: 30 start-page: 184 year: 2012 end-page: 189 ident: bib31 publication-title: Nat. Biotechnol. contributor: fullname: Junutula – volume: 28 start-page: 14441 year: 2012 end-page: 14449 ident: bib14 publication-title: Langmuir contributor: fullname: Ling – volume: 63 start-page: 1999 year: 2003 end-page: 2004 ident: bib32 publication-title: Cancer Res. contributor: fullname: Richards-Kortum – volume: 110 start-page: 5973 year: 2006 end-page: 5977 ident: bib18 publication-title: J. Phys. Chem. A contributor: fullname: Schatz – volume: 8 start-page: 3017 year: 2014 end-page: 3122 ident: bib6 publication-title: ACS Nano contributor: fullname: Lévy – volume: 4 start-page: 1029 year: 2004 end-page: 1034 ident: bib15 publication-title: Nano Lett. contributor: fullname: Van Duyne – volume: 114 start-page: 5515 year: 2010 end-page: 5519 ident: bib24 publication-title: J. Phys. Chem. A contributor: fullname: Etchegoin – volume: 22 start-page: 47 year: 2004 end-page: 52 ident: bib2 publication-title: Nat. Biotechnol. contributor: fullname: Alivisatos – volume: 1 start-page: 17 year: 2008 end-page: 32 ident: bib7 publication-title: Nanotechnol. Sci. Appl. contributor: fullname: Sun – volume: 42 start-page: 5880 year: 2013 end-page: 5906 ident: bib9 publication-title: Chem. Soc. Rev. contributor: fullname: Zadeha – volume: 3 start-page: 684 year: 2012 ident: bib3 publication-title: Nat. Commun. contributor: fullname: Hillenbrand – volume: 17 start-page: 1015 year: 2011 end-page: 1019 ident: bib8 publication-title: Nat. Med. contributor: fullname: Sia – volume: 5 start-page: 2246 year: 2005 end-page: 2252 ident: bib28 publication-title: Nano. Lett. contributor: fullname: Liphardt – volume: 60 start-page: 167 year: 2009 end-page: 192 ident: bib22 publication-title: Annu. Rev. Phys. Chem. contributor: fullname: Xia – volume: 442 start-page: 368 year: 2006 end-page: 373 ident: bib37 publication-title: Nature contributor: fullname: Whitesides – volume: 4 start-page: 1576 year: 2008 end-page: 1599 ident: bib20 publication-title: Small contributor: fullname: Tsukruk – volume: 17 start-page: 901 year: 1999 end-page: 905 ident: bib27 publication-title: Nat. Biotechnol. contributor: fullname: Hinterdorfer – volume: 111 start-page: 13794 year: 2007 end-page: 13803 ident: bib21 publication-title: J. Phys. Chem. C contributor: fullname: Etchegoin – volume: 15 start-page: 16 year: 2012 end-page: 25 ident: bib30 publication-title: Mater. Today contributor: fullname: Van Duyne – volume: 20 start-page: 319 year: 2000 end-page: 328 ident: bib1 publication-title: Methods contributor: fullname: Yu – volume: 102 start-page: 14587 year: 2005 end-page: 14592 ident: bib5 publication-title: PNAS contributor: fullname: Gerber – volume: 8 start-page: 1377 year: 2008 end-page: 1383 ident: bib26 publication-title: Cryst. Growth Des. contributor: fullname: Macagno – volume: 64 start-page: 39 year: 2011 end-page: 44 ident: bib33 publication-title: Phys. Today contributor: fullname: Stockman – volume: 10 start-page: 3006 year: 2010 end-page: 3012 ident: bib4 publication-title: Nano Lett. contributor: fullname: Feldmann – volume: 23 start-page: 741 year: 2005 end-page: 745 ident: bib35 publication-title: Nat. Biotechnol. contributor: fullname: Alivisatos – volume: 321 start-page: 388 year: 2008 end-page: 392 ident: bib11 publication-title: Science contributor: fullname: Dlott – volume: 7 start-page: 2080 year: 2007 end-page: 2088 ident: bib17 publication-title: Nano Lett. contributor: fullname: El-Sayed – volume: 66 start-page: 497 year: 2015 end-page: 503 ident: bib25 publication-title: Biosens. Bioelectron. contributor: fullname: Sim – volume: 10 start-page: 791 year: 2007 end-page: 799 ident: bib34 publication-title: Nat. Rev. Cancer contributor: fullname: Caldas – volume: 61 start-page: 907 year: 2002 end-page: 914 ident: bib13 publication-title: Microelectron. Eng. contributor: fullname: Fujii – volume: 112 start-page: 18849 year: 2008 end-page: 18859 ident: bib19 publication-title: J. Phys. Chem. C. Nanomater. Interfaces contributor: fullname: Vo-Dinh – volume: 28 start-page: 14441 year: 2012 ident: 10.1016/j.bios.2015.03.064_bib14 publication-title: Langmuir doi: 10.1021/la302795r contributor: fullname: Goh – volume: 110 start-page: 5973 year: 2006 ident: 10.1016/j.bios.2015.03.064_bib18 publication-title: J. Phys. Chem. A doi: 10.1021/jp0610867 contributor: fullname: Jensen – volume: 8 start-page: 1377 year: 2008 ident: 10.1016/j.bios.2015.03.064_bib26 publication-title: Cryst. Growth Des. doi: 10.1021/cg7009644 contributor: fullname: Pérez – volume: 112 start-page: 18849 year: 2008 ident: 10.1016/j.bios.2015.03.064_bib19 publication-title: J. Phys. Chem. C. Nanomater. Interfaces doi: 10.1021/jp8054747 contributor: fullname: Khoury – volume: 60 start-page: 167 year: 2009 ident: 10.1016/j.bios.2015.03.064_bib22 publication-title: Annu. Rev. Phys. Chem. doi: 10.1146/annurev.physchem.040808.090434 contributor: fullname: Lu – volume: 17 start-page: 901 year: 1999 ident: 10.1016/j.bios.2015.03.064_bib27 publication-title: Nat. Biotechnol. doi: 10.1038/12898 contributor: fullname: Raab – volume: 111 start-page: 13794 year: 2007 ident: 10.1016/j.bios.2015.03.064_bib21 publication-title: J. Phys. Chem. C doi: 10.1021/jp0687908 contributor: fullname: Le Ru – volume: 42 start-page: 5880 year: 2013 ident: 10.1016/j.bios.2015.03.064_bib9 publication-title: Chem. Soc. Rev. doi: 10.1039/c3cs35515b contributor: fullname: Chrimes – volume: 10 start-page: 791 year: 2007 ident: 10.1016/j.bios.2015.03.064_bib34 publication-title: Nat. Rev. Cancer doi: 10.1038/nrc2212 contributor: fullname: Stingl – volume: 10 start-page: 3006 year: 2010 ident: 10.1016/j.bios.2015.03.064_bib4 publication-title: Nano Lett. doi: 10.1021/nl101454a contributor: fullname: Ba – volume: 15 start-page: 16 year: 2012 ident: 10.1016/j.bios.2015.03.064_bib30 publication-title: Mater. Today doi: 10.1016/S1369-7021(12)70017-2 contributor: fullname: Sharma – volume: 63 start-page: 1999 year: 2003 ident: 10.1016/j.bios.2015.03.064_bib32 publication-title: Cancer Res. contributor: fullname: Sokolov – volume: 9 start-page: 2140 year: 2008 ident: 10.1016/j.bios.2015.03.064_bib12 publication-title: Chem. Phys.Chem. doi: 10.1002/cphc.200800349 contributor: fullname: Franke – volume: 22 start-page: 47 year: 2004 ident: 10.1016/j.bios.2015.03.064_bib2 publication-title: Nat. Biotechnol. doi: 10.1038/nbt927 contributor: fullname: Alivisatos – volume: 6 start-page: 439 year: 2011 ident: 10.1016/j.bios.2015.03.064_bib10 publication-title: Nat. Protoc. doi: 10.1038/nprot.2011.304 contributor: fullname: Dixit – volume: 20 start-page: 319 year: 2000 ident: 10.1016/j.bios.2015.03.064_bib1 publication-title: Methods doi: 10.1006/meth.1999.0925 contributor: fullname: Adamczyk – volume: 4 start-page: 1576 year: 2008 ident: 10.1016/j.bios.2015.03.064_bib20 publication-title: Small doi: 10.1002/smll.200800337 contributor: fullname: Ko – volume: 30 start-page: 184 year: 2012 ident: 10.1016/j.bios.2015.03.064_bib31 publication-title: Nat. Biotechnol. doi: 10.1038/nbt.2108 contributor: fullname: Shen – volume: 4 start-page: 1029 year: 2004 ident: 10.1016/j.bios.2015.03.064_bib15 publication-title: Nano Lett. doi: 10.1021/nl049670j contributor: fullname: Haes – volume: 442 start-page: 368 year: 2006 ident: 10.1016/j.bios.2015.03.064_bib37 publication-title: Nature doi: 10.1038/nature05058 contributor: fullname: Whitesides – volume: 114 start-page: 5515 year: 2010 ident: 10.1016/j.bios.2015.03.064_bib24 publication-title: J. Phys. Chem. A doi: 10.1021/jp100669q contributor: fullname: Meyer – volume: 1 start-page: 17 year: 2008 ident: 10.1016/j.bios.2015.03.064_bib7 publication-title: Nanotechnol. Sci. Appl. doi: 10.2147/NSA.S3788 contributor: fullname: Cai – volume: 17 start-page: 1015 year: 2011 ident: 10.1016/j.bios.2015.03.064_bib8 publication-title: Nat. Med. doi: 10.1038/nm.2408 contributor: fullname: Chin – volume: 5 start-page: 2246 year: 2005 ident: 10.1016/j.bios.2015.03.064_bib28 publication-title: Nano. Lett. doi: 10.1021/nl051592s contributor: fullname: Reinhard – volume: 7 start-page: 2080 year: 2007 ident: 10.1016/j.bios.2015.03.064_bib17 publication-title: Nano Lett. doi: 10.1021/nl071008a contributor: fullname: Jain – volume: 61 start-page: 907 year: 2002 ident: 10.1016/j.bios.2015.03.064_bib13 publication-title: Microelectron. Eng. doi: 10.1016/S0167-9317(02)00494-X contributor: fullname: Fujii – volume: 23 start-page: 741 year: 2005 ident: 10.1016/j.bios.2015.03.064_bib35 publication-title: Nat. Biotechnol. doi: 10.1038/nbt1100 contributor: fullname: Sönnichsen – volume: 50 start-page: 9737 year: 2014 ident: 10.1016/j.bios.2015.03.064_bib23 publication-title: Chem. Commun. doi: 10.1039/C4CC03734K contributor: fullname: Ma – volume: 66 start-page: 497 year: 2015 ident: 10.1016/j.bios.2015.03.064_bib25 publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2014.12.015 contributor: fullname: Nguyen – volume: 8 start-page: 3017 year: 2014 ident: 10.1016/j.bios.2015.03.064_bib6 publication-title: ACS Nano contributor: fullname: Bogart – volume: 3 start-page: 1479 year: 2012 ident: 10.1016/j.bios.2015.03.064_bib29 publication-title: J. Phys. Chem. Lett. doi: 10.1021/jz300426p contributor: fullname: Ringe – volume: 5 start-page: 10794 year: 2013 ident: 10.1016/j.bios.2015.03.064_bib36 publication-title: Nanoscale doi: 10.1039/c3nr02924g contributor: fullname: Wei – volume: 102 start-page: 14587 year: 2005 ident: 10.1016/j.bios.2015.03.064_bib5 publication-title: PNAS doi: 10.1073/pnas.0504917102 contributor: fullname: Backmann – volume: 64 start-page: 39 year: 2011 ident: 10.1016/j.bios.2015.03.064_bib33 publication-title: Phys. Today doi: 10.1063/1.3554315 contributor: fullname: Stockman – volume: 321 start-page: 388 year: 2008 ident: 10.1016/j.bios.2015.03.064_bib11 publication-title: Science doi: 10.1126/science.1159499 contributor: fullname: Fang – volume: 3 start-page: 684 year: 2012 ident: 10.1016/j.bios.2015.03.064_bib3 publication-title: Nat. Commun. doi: 10.1038/ncomms1674 contributor: fullname: Alonso-González
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Snippet	The length of bioreceptors plays an important role in signal enhancement of surface-enhanced Raman scattering (SERS) due to amplification of electromagnetic...
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SubjectTerms	Animals Antigens Biomarkers, Tumor - analysis Biomarkers, Tumor - immunology Cancer biomarkers Complex Mixtures - immunology Deposition Devices Equipment Design Equipment Failure Analysis Humans Immunoassay - instrumentation Immunoglobulin Fab Fragments - immunology Immunoglobulin G - immunology Immunosensors Lab-On-A-Chip Devices Microarray Analysis - instrumentation Microfluidics Multiplex detection Multiplexing Plasmonic nanoparticles Raman scattering Reproducibility of Results Sensitivity and Specificity SERS Silver Surface Plasmon Resonance - instrumentation
Title	Fabrication of plasmon length-based surface enhanced Raman scattering for multiplex detection on microfluidic device
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