Activation of Cytotoxic Lymphocytes and Presence of Regulatory T Cells in the Trachea of Non-Vaccinated and Vaccinated Chickens as a Recall to an Infectious Laryngotracheitis Virus (ILTV) Challenge

• Published in: Vaccines (Basel) Vol. 9; no. 8; p. 865
• Main Authors: Maekawa, Daniel, Riblet, Sylva M, Whang, Patrick, Hurley, David J, Garcia, Maricarmen
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05-08-2021; MDPI
• Subjects: Antigens; Bronchitis; Cell activation; Cell culture; chicken embryo origin (CEO); Chickens; cytotoxic T lymphocytes (CTLs); Cytotoxicity; Eggs; Embryos; Homeostasis; Immune response; Immune response (cell-mediated); Immune system; Immunoregulation; infectious laryngotracheitis virus (ILTV); Influenza; Laryngotracheitis; Larynx; Lymphocytes; Lymphocytes T; natural killer (NK) cells; Natural killer cells; Pathogens; Population; Poultry; recombinant herpesvirus of Turkey-laryngotracheitis (rHVT-LT); Respiration; Respiratory tract; T cell receptors; Tissue culture; tissue culture origin (TCO); Trachea; Vaccines; Viral infections; Virulence; Viruses; United States > US; regulatory T cells (Tregs); infectious laryngotracheitis virus (ILTV); protection; recombinant herpesvirus of Turkey-laryngotracheitis (rHVT-LT); tissue culture origin (TCO); natural killer (NK) cells; cytotoxic T lymphocytes (CTLs); chicken embryo origin (CEO)
• ISSN: 2076-393X; 2076-393X
• DOI: 10.3390/vaccines9080865

While the protective efficacy of the infectious laryngotracheitis virus (ILTV) vaccines is well established, little is known about which components of the immune response are associated with effective resistance and vaccine protection. Early studies have pointed to the importance of the T cell-mediated immune responses. This study aimed to evaluate the activation of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells and to quantify the presence of regulatory T cells (Tregs) in the larynx-trachea of chickens vaccinated with chicken embryo origin (CEO), tissue culture origin (TCO) and recombinant Herpesvirus of Turkey-laryngotracheitis (rHVT-LT) vaccines after challenge. Our results indicated that CEO vaccine protection was characterized by early CTLs and activated CTLs enhanced responses. TCO and rHVT-LT protection were associated with a moderate increase in resting and activated CTLs followed by an enhanced NK cell response. Tregs increase was only detected in the non-vaccinated challenged group, probably to support healing of the severe trachea epithelial damage. Taken together, our results revealed main differences in the cellular immune responses elicited by CEO, TCO, and rHVT-LT vaccination in the upper respiratory tract after challenge, and that activated CTLs rather than NK cells play a main role in vaccine protection.