Nucleotide Sequences of Gene Segments Encoding Membrane Domains of Immunoglobulin γ Chains

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 79; no. 8; pp. 2623 - 2627
• Main Authors: Yamawaki-Kataoka, Yuriko, Nakai, Sumiko, Miyata, Takashi, Honjo, Tasuku
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-04-1982; National Acad Sciences
• Subjects: Amino Acid Sequence; Amino acids; Animals; Antigens, Surface - immunology; Base Sequence; Cell Membrane - immunology; Cells; Cloning, Molecular; DNA Restriction Enzymes; DNA, Recombinant; Exons; Genes; Immunoglobulin gamma-Chains - genetics; Immunoglobulin Heavy Chains - genetics; Immunoglobulins; Introns; Mice; Nuclear membrane; Nucleotide sequences; P branes; Receptors
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.79.8.2623

The nucleotide sequences of the exons encoding membrane-bound IgG1, IgG2a, and IgG2b of mouse were determined and compared with the sequence of membrane-bound IgM. The sequences indicate that membrane-bound γ chains bear an additional 71 residues at the COOH termini, including 17-residue acidic, 26-residue hydrophobic, and 28-residue hydrophilic portions. The hydrophobic portion, which seems to be anchored in the lipid bilayer of the membrane, is highly conserved among membrane-bound μ and γ chains. We propose the presence of a membrane protein that recognizes the conserved hydrophobic segment and anchors the membrane-bound immunoglobulin. Comparison of the nucleotide sequences revealed another example of intervening sequence-mediated domain exchange around one of the membrane exons.