Antillatoxin and kalkitoxin, ichthyotoxins from the tropical cyanobacterium Lyngbya majuscula, induce distinct temporal patterns of NMDA receptor-mediated neurotoxicity

• Published in: Toxicon (Oxford) Vol. 37; no. 11; pp. 1645 - 1648
• Main Authors: Berman, F.W, Gerwick, W.H, Murray, T.F
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01-11-1999; Elsevier Science
• Subjects: Animals; antillatoxin; Antineoplastic Agents - antagonists & inhibitors; Antineoplastic Agents - toxicity; Bacteriology; Biological and medical sciences; Cells, Cultured; Cerebellum - cytology; Cerebellum - drug effects; curacin A; Cyanobacteria - chemistry; Cyanophyta; Cyclopropanes - antagonists & inhibitors; Cyclopropanes - toxicity; Dextrorphan - pharmacology; Dizocilpine Maleate - pharmacology; Excitatory Amino Acid Antagonists - pharmacology; Fundamental and applied biological sciences. Psychology; ichthyotoxins; kalkitoxin; Lethal Dose 50; Lipids - antagonists & inhibitors; Lipids - toxicity; Lipopeptides; Lipoproteins - antagonists & inhibitors; Lipoproteins - toxicity; Lyngbya majuscula; Marine Toxins - antagonists & inhibitors; Marine Toxins - chemistry; Microbiology; MK-801; NDMA receptor antagonists; Neurons - drug effects; Neuroprotective Agents - pharmacology; Neurotoxins - toxicity; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Peptides, Cyclic; Rats; Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors; Receptors, N-Methyl-D-Aspartate - drug effects; Thiazoles - antagonists & inhibitors; Thiazoles - toxicity; Cerebellum; Rat; Toxicity; Rodentia; Central nervous system; Nervous system; Glutamate receptor; In vitro; Biological activity; Marine environment; Toxin; Vertebrata; Mammalia; Cyanobacteria; Neurotoxin; Bacteria; NMDA receptor; Brain (vertebrata)
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/S0041-0101(99)00108-7

Curacin-A, antillatoxin and kalkitoxin, natural products from the marine cyanobacterium
Lyngbya majuscula, were tested for neurotoxicity in primary cultures of rat cerebellar granule neurons. Curacin-A was non-toxic, whereas antillatoxin and kalkitoxin produced concentration-dependent cytotoxicity with LC
50 values of 20.1±6.4 and 3.86±1.91 nM, respectively. Antillatoxin neurotoxicity was produced acutely, whereas kalkitoxin caused a delayed neurotoxic response. The cytotoxicity produced by both antillatoxin and kalkitoxin was prevented by the non-competitive NMDA receptor antagonists dextrorphan and MK-801.