Different effects of sonoporation on cell morphology and viability

• Published in: Biomolecules & biomedicine Vol. 12; no. 2; pp. 64 - 68
• Main Authors: Zhang, Ji-Zhen, Saggar, Jasdeep K, Zhou, Zhao-Li, Hu, Bing
• Format: Journal Article
• Language: English
• Published: Bosnia and Herzegovina Association of Basic Medical Sciences 01-05-2012; Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
• Subjects: Cell Line, Tumor; Cell physiology; Cell Survival - physiology; Cells - ultrastructure; Culture Media; drug delivery; Fluoresceins - metabolism; Health aspects; Humans; low frequency ultrasound; Male; microbubble contrast agents; Microbubbles; Microscopy, Electron, Scanning; Microscopy, Fluorescence; molecular delivery; Morphology; Patient outcomes; Sonication; sonoporation; Ultrasonics; Ultrasonics in medicine; ultrasound; Bosnia and Herzegovina
• ISSN: 1512-8601; 2831-0896; 1840-4812; 2831-090X
• DOI: 10.17305/bjbms.2012.2497

The objective of our study was to investigate changes in cell morphology and viability after sonoporation. Sonoportion was achieved by ultrasound (21 kHz) exposure on adherent human prostate cancer DU145 cells in the cell culture dishes with the presence of microbubble contrast agents and calcein (a cell impermeant dye). We investigated changes in cell morphology immediately after sonoporation under scanning electron microscope (SEM) and changes in cell viability immediately and 6 h after sonoporation under fluorescence microscope. It was shown that various levels of intracellular calcein uptake and changes in cell morphology can be caused immediately after sonoporation: smooth cell surface, pores in the membrane and irregular cell surface. Immediately after sonoporation, both groups of cells with high levels of calcein uptake and low levels of calcein uptake were viable; 6 h after sonoporation, group of cells with low levels of calcein uptake still remained viable, while group of cells with high levels of calcein uptake died. Sonoporation induces different effects on cell morphology, intracellular calcein uptake and cell viability.