Steroid profiles of transgenic tobacco expressing an Actinomyces 3-hydroxysteroid oxidase gene

The action of a 3-hydroxysteroid oxidase enzyme within mature vegetative transgenic tobacco alters the resident sterol pool facilitating the enhanced accumulation of sterol, 3-ketosteroid and stanol throughout the plant. The modulated steroid pool correlates directly with altered thylakoid membrane-...

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Bibliographic Details
Published in:	Phytochemistry (Oxford) Vol. 65; no. 22; pp. 2967 - 2976
Main Authors:	Heyer, Jennelle, Parker, Brandon, Becker, David, Ruffino, John, Fordyce, Amber, Witt, Matt De, Bedard, Mark, Grebenok, Robert
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Ltd 01-11-2004 Elsevier
Subjects:	3-Hydroxysteroid Dehydrogenases - metabolism 3-Hydroxysteroid oxidase 3-Ketosteroid Actinomyces Actinomyces - enzymology bacterial proteins Biological and medical sciences Campesterol Chemical constitution Chlorophyll - metabolism Chloroplast Chloroplasts - metabolism Cholesterol Electron Transport Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation, Plant lipid content Metabolism Metabolism. Physicochemical requirements Nicotiana - genetics Nicotiana - metabolism Nicotiana tabacum oxidoreductases Photosynthesis phytosterols Plant physiology and development Plants, Genetically Modified Sitosterol Stanol steroids Steroids - metabolism Stigmasterol Tobacco transgenes Transgenic transgenic plants Sitosterol Tobacco Stigmasterol Campesterol 3-Hydroxysteroid oxidase Transgenic Stanol 3-Ketosteroid Nicotiana tabacum Photosynthesis Cholesterol Chloroplast Steroid Campcsterol Whole plant Chlornplast In vitro Gene Dicotyledones Angiospermae Spermatophyta Transgenic plant Solanaceae Sterol
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