Impedimetric biosensor based on self-assembled hybrid cystein-gold nanoparticles and CramoLL lectin for bacterial lipopolysaccharide recognition

• Published in: Journal of colloid and interface science Vol. 362; no. 1; pp. 194 - 201
• Main Authors: Oliveira, Maria D.L., Andrade, Cesar A.S., Correia, Maria T.S., Coelho, Luana C.B.B., Singh, Pankaj R., Zeng, Xiangqun
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01-10-2011; Elsevier
• Subjects: acetates; Bacteria - isolation & purification; Bacterial lipopolysaccharide; Biosensing Techniques - methods; biosensors; Chemistry; Colloidal state and disperse state; Cratylia mollis; Cyclic voltammetry; Cysteine - chemistry; dielectric spectroscopy; Electric Impedance; Electrochemistry; Electrodes; electrostatic interactions; Endotoxins; Escherichia coli; Exact sciences and technology; ferricyanides; General and physical chemistry; gold; Gold - chemistry; Immobilized Proteins - chemistry; Impedance spectroscopy; iron; Klebsiella pneumoniae; lectins; Lectins - chemistry; lipopolysaccharides; Lipopolysaccharides - isolation & purification; Nanoparticles; Nanoparticles - chemistry; Physical and chemical studies. Granulometry. Electrokinetic phenomena; Salmonella enterica; Sensitivity and Specificity; Serratia marcescens; Surface physical chemistry; Cyclic voltammetry; Nanoparticles; Bacterial lipopolysaccharide; Impedance spectroscopy; Cratylia mollis; Endotoxins; Self assembly; Atomic force microscopy; Nanoparticle; Composite material; Electrodes; Chlorides; Maleic acid; Redox system; Charge transfer; Gold; Electrostatic interaction; Transition metal; Acetate; Contamination; Resistance; Biosensor; Electrochemistry; Impedance; Interface; Recognition
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2011.06.042

Effect of different concentrations of LPS from E. coli, S. enterica, S. marcescens and K. pneumoniae on ΔRCT during the interaction of the lectin-modified electrode with glycoconjugates. [Display omitted]
► A new strategy to construct an electrochemical biosensor for bacterial LPS has been described. ► AFM images showed that LPS was specifically recognized. ► A sensor for selective discrimination of LPS types with high sensitivity has been achieved. ► The biosensor provides an appropriate biomimetic interface for specific recognition of LPS.
We report the development of a new selective and specific electrochemical biosensor for bacterial lipolysaccharide (LPS). An electrode interface was constructed using a l-cysteine-gold nanoparticle (AuNpCys) composite to be immobilized by electrostatic interaction in the network of a poly(vinyl chloride-vinyl acetate maleic acid) (PVM) layer on a gold bare electrode. The impedimetric biosensor is fabricated by self-assembled CramoLL lectin on the PVM–AuNpCys–modified gold electrode through electrostatic interaction. CramoLL is used as the recognition interface. AFM images showed that LPS was specifically recognized on the PVM–AuNpCys–CramoLL system surface. The measurements of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) showed that the electrochemical response of a redox probe system (K4[Fe(CN)6]4−/K3[Fe(CN)6]3−) were blocked, due to the procedures of modified electrode with PVM–AuNpCys–CramoLL. In the majority of the experiments the lectin retained its activity as observed through its interaction with LPS from Escherichia coli, Serratia marcescens, Salmonella enterica and Klebsiella pneumoniae. The results are expressed in terms of the charge transfer resistance and current peak anodic using the EIS and CV techniques for the development of a biosensor for contamination by endotoxins. A new type of sensor for selective discrimination of LPS types with a high sensitivity has been obtained.